scholarly journals Effect of artemisinins and other endoperoxides on nitric oxide-related signaling pathway in RAW 264.7 mouse macrophage cells

Nitric Oxide ◽  
2008 ◽  
Vol 19 (2) ◽  
pp. 184-191 ◽  
Author(s):  
V. Badireenath Konkimalla ◽  
Martina Blunder ◽  
Bernhard Korn ◽  
Shahid A. Soomro ◽  
Herwig Jansen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathway ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Macrophage Cells

scholarly journals Effect of pecan phenolics on the release of nitric oxide from murine RAW 264.7 macrophage cells

2016 ◽  
Vol 212 ◽  
pp. 681-687 ◽  
Author(s):  
Katherine S. Robbins ◽  
Phillip Greenspan ◽  
Ronald B. Pegg
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

2009 ◽  
Vol 31 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Chikatoshi Kasugai ◽  
Akiko Morikawa ◽  
Yoshikazu Naiki ◽  
Naoki Koide ◽  
Takayuki Komatsu ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Macrophage Cells

scholarly journals Immunomodulatory Activity of Phyllanthus maderaspatensis in LPS-Stimulated Mouse Macrophage RAW 264.7 Cells

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 129
Author(s):  
Uoorakkottil Ilyas ◽  
Deepshikha P. Katare ◽  
Punnoth Poonkuzhi Naseef ◽  
Mohamed Saheer Kuruniyan ◽  
Muhammed Elayadeth-Meethal ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 Cells ◽  
Immunomodulatory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Significant Stimulatory Effect

Phyllanthus species (Family Euphorbiaceae) has been used in traditional medicine of several countries as a cure for numerous diseases, including jaundice and hepatitis. This study is an attempt to evaluate the immunomodulatory activity of various fractions, column eluents of ethyl acetate fraction, and their polyphenols. Phyllanthus maderaspatensis were standardized using high-performance liquid chromatography to identify and quantify polyphenols, and purification of polyphenols was carried out using vacuum liquid chromatography. Subsequently, we tested various fractions, column eluents of ethyl acetate fraction, and polyphenols in vitro to assess their impact on nitric oxide (NO) production in LPS-stimulated mouse macrophage RAW 264.7 cells. The ethyl acetate fraction (100 μg mL−1) had a more significant stimulatory effect on LPS-stimulated NO production by the RAW 264.7 cells. We found that the ethyl acetate fraction contains a high amount of catechin, quercetin, ellagic acid kaempferol, and rutin, which are responsible for immunomodulation. The ethyl acetate fraction at concentrations of 25 and 50 μg mL−1 had a significant inhibitory effect and 100 μg mL−1 had a more significant stimulatory effect when compared with the LPS control. The percentage of inhibition by LPS control ranged from zero percentage, kaempferol ranged from 45.4% at 50 μg mL−1 to 41.88% at 100 μg mL−1, catechin ranged from 50% at 50 μg mL−1 to 35.28% at 100 μg mL−1, rutin ranged from 36.2% at 50 μg mL−1 to 47.44% at 100 μg mL−1, gallic acid ranged from 28.4% at 50 μg mL−1 to 50.9% at 100 μg mL−1, ellagic acid ranged from 45.12% at 50 μg mL−1 to 38.64% at 100 μg mL−1, and purified quercetin ranged from 26.2% at 50 μg mL−1to 45.48% at 100 μg mL−1. As NO plays an important role in the immune function, polyphenols’ treatment could modulate several aspects of host defense mechanisms owing to the stimulation of the inducible nitric oxide synthase.

Different action of IBMX, isoproterenol and rutin on orthovanadate-induced nitric oxide release in mouse macrophage cells

2002 ◽  
Vol 50 (3) ◽  
pp. 323-341
Author(s):  
S. Koncz ◽  
Edit J. Horváth
Keyword(s):  
Nitric Oxide ◽  
Adrenergic Receptor ◽  
No Production ◽  
Mouse Macrophage ◽  
Nitric Oxide Release ◽  
Macrophage Cells ◽  
Reverse Mode ◽  
Dichlorofluorescein Diacetate ◽  
Superoxide Scavenger ◽  
Adrenergic Receptor Agonist

The effects of cAMP-elevating compounds IBMX (3-isobutyl-1-methyl­xanthine) and isoproterenol, and that of rutin (an effective superoxide scavenger) were studied on orthovanadate- (a putative protein-phosphotyrosine phosphatase inhibitor) induced nitric oxide (NO) production in J774A.1 mouse macrophage cells. As we previously reported (Koncz and Horváth, 2000), rutin and sodium orthovanadate act synergistically to induce production of high amount of NO in J774A.1 cells. IBMX, an agent that can elevate cAMP level in the cells, can reduce the production of both the LPS- and rutin + orthovanadate-induced NO in macrophages. In contrast, isoproterenol, a non-selective ß-adrenergic receptor agonist, that reduced the LPS-induced NO production in macrophage cells, was unable to reduce the rutin + orthovanadate-induced NO production without negatively affecting cell viability. Moreover, isoproterenol dramatically enhanced the orthovanadate-induced NO synthesis in J774A.1 cells. Our previous study clarified that rutin and orthovanadate, in a specific concentration ratio of both, were able to produce hydrogen peroxide (H2O2). Using 2',7'-dichlorofluorescein-diacetate as a marker for H2O2, isoproterenol alone induced its oxidation but the rutin plus orthovanadate-induced H2O2 production was reduced by isoproterenol. These observations have revealed that, in some cases, H2O2 and superoxide (O2-) scavengers can act in a reverse mode on macrophage cells depending on the presence or absence of orthovanadate.

scholarly journals Candida albicans Killing by RAW 264.7 Mouse Macrophage Cells: Effects of Candida Genotype, Infection Ratios, and Gamma Interferon Treatment

2002 ◽  
Vol 70 (11) ◽  
pp. 6319-6329 ◽  
Author(s):  
A. Marcil ◽  
D. Harcus ◽  
D. Y. Thomas ◽  
M. Whiteway
Keyword(s):  
Candida Albicans ◽  
Cell Line ◽  
Systemic Infection ◽  
Gamma Interferon ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Wild Type ◽  
Macrophage Cells ◽  
Dilution Assay ◽  
Infection Models

ABSTRACT Phagocytic cells such as neutrophils and macrophages are potential components of the immune defense that protects mammals against Candida albicans infection. We have tested the interaction between the mouse macrophage cell line RAW 264.7 and a variety of mutant strains of C. albicans. We used an end point dilution assay to monitor the killing of C. albicans at low multiplicities of infection (MOIs). Several mutants that show reduced virulence in mouse systemic-infection models show reduced colony formation in the presence of macrophage cells. To permit analysis of the macrophage-Candida interaction at higher MOIs, we introduced a luciferase reporter gene into wild-type and mutant Candida cells and used loss of the luminescence signal to quantify proliferation. This assay gave results similar to those for the end point dilution assay. Activation of the macrophages with mouse gamma interferon did not enhance anti-Candida activity. Continued coculture of the Candida and macrophage cells eventually led to death of the macrophages, but for the RAW 264.7 cell line this was not due to apoptotic pathways involving caspase-8 or -9 activation. In general Candida cells defective in the formation of hyphae were both less virulent in animal models and more sensitive to macrophage engulfment and growth inhibition. However the nonvirulent, hypha-defective cla4 mutant line was considerably more resistant to macrophage-mediated inhibition than the wild-type strain. Thus although mutants sensitive to engulfment are typically less virulent in systemic-infection models, sensitivity to phagocytic macrophage cells is not the unique determinant of C. albicans virulence.

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