Rapid Detection of Complementary-and Mismatched DNA Sequences Using Fluorescence Polarization

1996 ◽  
Vol 29 (10) ◽  
pp. 1741-1749 ◽  
Author(s):  
Makoto Tsuruoka ◽  
Kazuyoshi Yano ◽  
Kazunori Ikebukuro ◽  
Scott McNiven ◽  
Toshifumi Takeuchi ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Mismatched Dna

The rapid detection of microRNA based on p19-enhanced fluorescence polarization

2014 ◽  
Vol 50 (47) ◽  
pp. 6236-6239 ◽  
Author(s):  
Yuan-Chen He ◽  
Bin-Cheng Yin ◽  
Lihua Jiang ◽  
Bang-Ce Ye
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Polarization Method ◽  
Enhanced Fluorescence ◽  
Microrna Detection

Viaa molecular caliper p19 protein, we have developed an amplified fluorescence polarization method for rapid microRNA detection.

Classical and Quantum Mechanical Calculations of the Stacking Interaction of NdIII Complexes with Regular and Mismatched DNA Sequences

2019 ◽  
Vol 123 (15) ◽  
pp. 3219-3231
Author(s):  
María J. Beltrán-Leiva ◽  
Isabel Fuenzalida-Valdivia ◽  
Plinio Cantero-López ◽  
Ana Bulhões-Figueira ◽  
Jans Alzate-Morales ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Quantum Mechanical ◽  
Quantum Mechanical Calculations ◽  
Stacking Interaction ◽  
Mismatched Dna

A highly specific and sensitive fluorescence polarization immunoassay for the rapid detection of triazophos residue in agricultural products

2016 ◽  
Vol 8 (36) ◽  
pp. 6636-6644 ◽  
Author(s):  
Ying Liu ◽  
Rui Liu ◽  
Anna Boroduleva ◽  
Sergei Eremin ◽  
Yirong Guo ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Agricultural Products ◽  
Fluorescence Polarization Immunoassay

A highly specific and sensitive fluorescence polarization immunoassay for the rapid detection of triazophos.

Development of DNA probes for the identification of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex

1995 ◽  
Vol 85 (3) ◽  
pp. 345-353 ◽  
Author(s):  
Maya B. Gunasekera ◽  
B.G.D.N.K. de Silva ◽  
W. Abeyewickreme ◽  
S.K. Subbarao ◽  
H.G. Nandadasa ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Rapid Detection ◽  
Copy Number ◽  
Blot Hybridization ◽  
Dna Probes ◽  
Anopheles Culicifacies ◽  
Dot Blot ◽  
Dot Blot Hybridization ◽  
Field Investigations ◽  
Highly Repetitive Dna

AbstractThree highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu lato. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.

scholarly journals Detection of SARS CoV-2 reinfections by rapid inexpensive techniques

2021 ◽  
Author(s):  
Sherko Subhan Niranji ◽  
Sirwan M.A. Al-Jaf
Keyword(s):  
Developing Countries ◽  
Dna Sequences ◽  
Rapid Detection ◽  
Phylogenetic Analyses ◽  
Immunocompetent Patient ◽  
Clinical Samples ◽  
Specific Primers ◽  
Persistent Infections ◽  
The World ◽  
The Uk

Abstract Reinfections of SARS CoV-2 are rare in the world and it is difficult to be confirmed whether it is a reinfection or persistent infection. The most prominent factors used for differentiating the reinfections from persistent infections are whole genome sequencings and phylogenetic analyses that require times and funds, which may not be feasible in most developing countries. We previously developed rapid economical methods to identify both D614G and N501Y mutations in clinical samples using rRT PCR probes and endpoint PCR specific primers. Current study has found an immunocompetent patient with a SARS CoV-2 N501Y reinfection without comorbidities. The results suggested that the initial infection was due to a variant contained only D614G mutation while the reinfection was potentially as result of the UK variant contained three mutations confirmed by DNA sequences, including D614G, N501Y and A570D mutations. Seven cases of reinfections were also confirmed by these methods suggested that these techniques will support rapid detection of SARS CoV-2 reinfections in developing countries where sequencing tools are unavailable.

Rapid detection and sequencing of specific in vitro amplified DNA sequences using solid phase methods

1990 ◽  
Vol 4 (4) ◽  
pp. 285-297 ◽  
Author(s):  
Johan Wahlberg ◽  
Joakim Lundeberg ◽  
Thomas Hultman ◽  
Martin Holmberg ◽  
Mathias Uhlén
Keyword(s):  
Dna Sequences ◽  
Rapid Detection ◽  
Solid Phase
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