The rapid detection of microRNA based on p19-enhanced fluorescence polarization

2014 ◽  
Vol 50 (47) ◽  
pp. 6236-6239 ◽  
Author(s):  
Yuan-Chen He ◽  
Bin-Cheng Yin ◽  
Lihua Jiang ◽  
Bang-Ce Ye
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Polarization Method ◽  
Enhanced Fluorescence ◽  
Microrna Detection

Viaa molecular caliper p19 protein, we have developed an amplified fluorescence polarization method for rapid microRNA detection.

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

2017 ◽  
Vol 89 (11) ◽  
pp. 5900-5908 ◽  
Author(s):  
Zhu Chen ◽  
Hui Li ◽  
Wenchao Jia ◽  
Xiaohui Liu ◽  
Zhoumin Li ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Enhanced Fluorescence

Optimization of a Fluorescence Polarization Immunoassay for Rapid Quantification of Deoxynivalenol in Durum Wheat–Based Products

2006 ◽  
Vol 69 (11) ◽  
pp. 2712-2719 ◽  
Author(s):  
VINCENZO LIPPOLIS ◽  
MICHELANGELO PASCALE ◽  
ANGELO VISCONTI
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Durum Wheat ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay ◽  
Polarization Method ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Rapid Quantification

A fluorescence polarization immunoassay previously described for deoxynivalenol (DON) screening in wheat was optimized for the rapid quantification of DON in durum wheat kernels, semolina, and pasta. A background signal was observed in both spiked and naturally contaminated samples, strictly depending on the testing matrix. After subtracting the background DON level for durum wheat (0.27 μg of DON per g), semolina (0.08 μg of DON per g), and pasta (0.04 μg of DON per g), an accurate quantification of DON was possible at levels greater than 0.10 μg/g for all matrices. Average recoveries from spiked samples (0.25 to 1.75 μg/g) were 98, 102, and 101% for wheat, semolina, and pasta, respectively. Comparative analyses of 35 naturally contaminated durum wheat samples, 22 semolina samples, and 26 pasta samples performed by both the fluorescence polarization method and high-pressure liquid chromatography/immunoaffinity cleanup showed a good correlation (r > 0.995). The fluorescence polarization method showed better accuracy and precision with respect to the high-pressure liquid chromatography method and is suitable for the rapid and quantitative determination of DON in durum wheat–based products at levels foreseen by existing or coming international regulations.

scholarly journals Studies of the Agarose Gelling System by the Fluorescence Polarization Method. II.

1978 ◽  
Vol 10 (5) ◽  
pp. 485-494 ◽  
Author(s):  
Akira Hayashi ◽  
Kyosuke Kinoshita ◽  
Masaru Kuwano ◽  
Atsumi Nose
Keyword(s):  
Fluorescence Polarization ◽  
Polarization Method

Black phosphorus nanosheets for rapid microRNA detection

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5060-5064 ◽  
Author(s):  
Jie Zhou ◽  
Zhongjun Li ◽  
Ming Ying ◽  
Maixian Liu ◽  
Xiaomei Wang ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Rapid Detection ◽  
Black Phosphorus ◽  
Sensing Platform ◽  
Microrna Detection ◽  
First Time

Herein, for the first time, a sensitive sensing platform for rapid detection of microRNA was developed by employing black phosphorus nanosheets as the fluorescence quenching material.

Rapid Detection of Complementary-and Mismatched DNA Sequences Using Fluorescence Polarization

1996 ◽  
Vol 29 (10) ◽  
pp. 1741-1749 ◽  
Author(s):  
Makoto Tsuruoka ◽  
Kazuyoshi Yano ◽  
Kazunori Ikebukuro ◽  
Scott McNiven ◽  
Toshifumi Takeuchi ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Fluorescence Polarization ◽  
Rapid Detection ◽  
Mismatched Dna
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