Interpretation of Serologic Tests for Mycoplasma gallisepticum

1973 ◽  
Vol 29 (4) ◽  
pp. 345-353 ◽  
Author(s):  
H. E. Adler ◽  
A. D. Wiggins
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Serologic Tests

Comparison of Culture, PCR, and Different Serologic Tests for Detection of Mycoplasma gallisepticum and Mycoplasma synoviae Infections

2005 ◽  
Vol 49 (2) ◽  
pp. 260-268 ◽  
Author(s):  
A. Feberwee ◽  
D. R. Mekkes ◽  
J. J. de Wit ◽  
E. G. Hartman ◽  
A. Pijpers
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Mycoplasma Synoviae ◽  
Serologic Tests

Evaluation of Serologic Tests for Mycoplasma gallisepticum in Wild Turkeys

1985 ◽  
Vol 21 (1) ◽  
pp. 58-61 ◽  
Author(s):  
Tonie E. Rocke ◽  
Thomas M. Yuill ◽  
Terry E. Amundson
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Serologic Tests ◽  
Wild Turkeys

scholarly journals GAA Trinucleotide Repeat Region Regulates M9/pMGA Gene Expression in Mycoplasma gallisepticum

2000 ◽  
Vol 68 (2) ◽  
pp. 871-876 ◽  
Author(s):  
Li Liu ◽  
Kevin Dybvig ◽  
Victor S. Panangala ◽  
Vicky L. van Santen ◽  
Christopher T. French
Keyword(s):  
Gene Expression ◽  
Trinucleotide Repeat ◽  
Mycoplasma Gallisepticum ◽  
Avian Host ◽  
Phase Variable ◽  
Repeat Region ◽  
Chromosomal Dna ◽  
Promoter Regions ◽  
Variable Expression ◽  
Gaa Repeats

ABSTRACT Mycoplasma gallisepticum, the cause of chronic respiratory infections in the avian host, possesses a family of M9/pMGA genes encoding an adhesin(s) associated with hemagglutination. Nucleotide sequences of M9/pMGA gene family members indicate extensive sequence similarity in the promoter regions of both the transcribed and silent genes. The mechanism that regulates M9/pMGA gene expression is unknown, but studies have revealed an apparent correlation between gene expression and the number of tandem GAA repeat motifs located upstream of the putative promoter. In this study, transposon Tn4001was used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of the GAA repeats in M9/pMGA gene expression in M. gallisepticum. A 336-bp M9 gene fragment (containing the GAA repeat region, the promoter, and the translation start codon) was amplified by PCR, ligated with alacZ gene from E. coli, and inserted into the Tn4001-containing plasmid pISM2062. This construct was transformed into M. gallisepticum PG31. Transformants were filter cloned on agar supplemented with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color selection. Several cycles of filter cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off states in successive generations of progeny clones. The promoter regions of the M9-lacZ hybrid genes of individual progeny clones were amplified by PCR and sequenced. The only differences between the promoter regions of the blue and white colonies were in the number of GAA repeats. Clones that expressedlacZ had exactly 12 tandem copies of the GAA repeat. Clones that did not express lacZ invariably had either more than 12 (14 to 16) or fewer than 12 (5 to 11) GAA repeats. Southern analysis of M. gallisepticum chromosomal DNA confirmed that the phase-variable expression of the lacZ reporter gene was not caused by Tn4001 transposition. These data strongly indicate that changes in the length of the GAA repeat region are responsible for regulating M9/pMGA gene expression.

scholarly journals SARS-CoV-2 serologic tests: do not forget the good laboratory practice

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Elena Aloisio ◽  
Felicia Stefania Falvella ◽  
Assunta Carnevale ◽  
Mauro Panteghini
Keyword(s):  
Good Laboratory Practice ◽  
Laboratory Practice ◽  
Serologic Tests ◽  
Good Laboratory

scholarly journals Validation of COVID-19 serologic tests and large scale screening of asymptomatic healthcare workers

2021 ◽  
Author(s):  
Kristin E. Mullins ◽  
VeRonika Merrill ◽  
Matthew Ward ◽  
Brent King ◽  
Peter Rock ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Large Scale ◽  
Serologic Tests ◽  
Large Scale Screening

scholarly journals Early Confirmation of Mycoplasma pneumoniae Infection by Two Short-Term Serologic IgM Examination

Diagnostics ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 353
Author(s):  
Ha Eun Jeon ◽  
Hyun Mi Kang ◽  
Eun Ae Yang ◽  
Hye Young Han ◽  
Seung Beom Han ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Early Stage ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Short Term ◽  
Serologic Tests ◽  
Polymerase Chain ◽  
Positive Correlations ◽  
Patient Selection Bias ◽  
Mycoplasma Pneumoniae Infection

The aim of the present study is to re-evaluate the clinical application of two-times serologic immunoglobulin M (IgM) tests using microparticle agglutination assay (MAA), an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assay in diagnosing Mycoplasma pneumoniae (MP) infection. A retrospective analysis of 62 children with MP pneumonia during a recent epidemic (2019–2020) was conducted. The MAA and ELISA immunoglobulin M (IgM) and IgG measurements were conducted twice at admission and around discharge, and MP PCR once at presentation. Diagnostic rates in each test were calculated at presentation and at discharge. The seroconverters were 39% (24/62) of patients tested by MAA and 29% (18/62) by ELISA. At presentation, the diagnostic positive rates of MAA, ELISA, and PCR tests were 61%, 71%, and 52%, respectively. After the second examination, the rates were 100% in both serologic tests. There were positive correlations between the titers of MAA and the IgM values of ELISA. The single serologic IgM or PCR tests had limitations to select patients infected with MP in the early stage. The short-term, paired IgM serologic tests during hospitalization can reduce patient-selection bias in MP infection studies.

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