Effects of temperature and humidity on the development of eggs ofToxocara canisunder laboratory conditions
AbstractThe influence of temperature and humidity on the survival and development ofToxocara caniseggs in anin vitromodel system was investigated. Two soil samples were inoculated withT. caniseggs and maintained at 3% and 50% humidity and temperatures of 19–24°C. Nine soil samples were inoculated withT. caniseggs of which three samples were kept at 4°C with humidities at 3%, 15%, and 30%; three were maintained at 21°C and three more were incubated at 34°C, and at the same three humidity levels. Samples were monitored every 7 days for a total of 2 months, for the presence and development of eggs. With increasing temperature, the number of eggs undergoing development increased (P<0.01); the number of deformed eggs decreased, the number of infective eggs increased (P<0.01), and egg maturation was accelerated. A decrease in the survival of infective eggs occurred at 34°C. An increase in humidity produced a rise in the number of developed eggs at all three temperatures (P<0.01). This study suggests that elevated temperatures accelerated the development as well as the degradation of eggs ofT. canis, whereas the range in humidity was directly correlated with egg development.