Granulomatous inflammation duringHeligmosomoides polygyrusprimary infections in FVB mice

2004 ◽  
Vol 78 (1) ◽  
pp. 17-24 ◽  
Author(s):  
A. Cywińska ◽  
K. Czumińska ◽  
A. Schollenberger
Keyword(s):  
Lymph Nodes ◽  
Intestinal Mucosa ◽  
Post Infection ◽  
Egg Production ◽  
Granulomatous Inflammation ◽  
Fast Response ◽  
Intraepithelial Lymphocytes ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Worm Expulsion

AbstractHost responses to primary infections withHeligmosomoides polygyruswere studied in fast responding FVB mice (H-2q). Pathological changes in the intestinal mucosa, mesenteric lymph nodes and spleen were examined. Features of the fast response were typical: low effectiveness of infection and limiting of parasite survival and egg production, with worm expulsion occurring about 60 days post-infection. The intestinal inflammatory response involved infiltration by different cells into the intestinal mucosa and granulomata formation. As is typical for intestinal nematode infection enteropathy, decreased villus:crypt ratio and hyperplasia of goblet and Paneth cells were also present. Reactions of the intestinal mucosa, mesenteric lymph nodes and spleen increased over time post-infection and after worm expulsion. Enteropathy may help worm expulsion by creating an unfavourable environment forH. polygyrus. The implications of these findings and the potential role of intestinal intraepithelial lymphocytes in the pathogenesis of generated lesions are discussed.

scholarly journals Analysis of Salmonella enterica Serotype-Host Specificity in Calves: Avirulence of S. enterica Serotype Gallinarum Correlates with Bacterial Dissemination from Mesenteric Lymph Nodes and Persistence In Vivo

2002 ◽  
Vol 70 (12) ◽  
pp. 6788-6797 ◽  
Author(s):  
Susan M. Paulin ◽  
Patricia R. Watson ◽  
Annette R. Benmore ◽  
Mark P. Stevens ◽  
Philip W. Jones ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Host Specificity ◽  
Intestinal Mucosa ◽  
Salmonella Enterica ◽  
Systemic Disease ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Oral Inoculation ◽  
Pass Through

ABSTRACT Host and bacterial factors that determine whether Salmonella serotypes remain restricted to the gastrointestinal tract or penetrate beyond the mucosa and cause systemic disease remain largely undefined. Here, factors influencing Salmonella host specificity in calves were assessed by characterizing the pathogenesis of different serotypes. Salmonella enterica serotype Dublin was highly virulent intravenously, whereas S. enterica serotype Choleraesuis was moderately virulent. Both serotypes were virulent in calves infected orally. In contrast, S. enterica serotypes Gallinarum and Abortusovis were avirulent by either route. Serotypes Dublin, Gallinarum, and Abortusovis colonized the intestinal tract 24 h after oral inoculation, yet only serotype Dublin was consistently recovered from systemic tissues. Serotypes Dublin and Gallinarum invaded bovine intestines in greater numbers and induced greater enteropathogenic responses than serotypes Choleraesuis and Abortusovis. However, only serotype Dublin was able to persist within the intestinal mucosa, and use of a novel cannulation model demonstrated that serotype Dublin was able to pass through the mesenteric lymph nodes in greater numbers than serotype Gallinarum. Together, these results suggest that initial interactions with the intestinal mucosa do not correlate with host specificity, although persistence within tissues and translocation via efferent lymphatics appear to be crucial for the induction of bovine salmonellosis.

scholarly journals Cavitation of mesenteric lymph nodes, splenic atrophy, and a flat small intestinal mucosa

1984 ◽  
Vol 87 (3) ◽  
pp. 606-614 ◽  
Author(s):  
C. Matuchansky ◽  
R. Colin ◽  
J. Hemet ◽  
G. Touchard ◽  
P. Babin ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Intestinal Mucosa ◽  
Small Intestinal Mucosa ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Small Intestinal ◽  
Splenic Atrophy

scholarly journals Progressive Pathological Changes in Sub-clinical Experimental Para-tuberculosis in Goats

2017 ◽  
Vol 34 ◽  
pp. 69-84
Author(s):  
U.M. Singh ◽  
B.N. Tripathi ◽  
O.P. Paliwal
Keyword(s):  
Lymph Nodes ◽  
Post Infection ◽  
Diagnostic Tests ◽  
Giant Cells ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Mesenteric Fat ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Histopathological Studies ◽  
Mucosal Thickening

An experiment was carried out to study the development of sequential histological lesions and efficacy of certain diagnostic tests in experimentally induced subclinical paratuberculosis infection in goats. Twelve goats of 8-12 weeks age were infected with 4.23 x 109 Mycobacterium avium subsp. Paratuberculosis on 8 occasions. Eight goats were kept as in-contact controls and 4 as uninfected controls. Diagnostic tests, gross and histopathological studies were carried out at 3, 6, 9 and 12 months post-infection. Two goats were positive in the AGID test at 12MPI. Three goats, one at 9 MPI and two at 12 MPI were positive in faecal culture. Tissue PCR detected one goat positive each at 9 and 12 MPI. Six (50%) of 12 infected goats had gross and histological lesions. Marked enlargements of the mesenteric lymph nodes were observed in the early stages (3 and 6 months post infection) of infection. Mucosal thickening and corrugation of the jejunal and ileal mucosae, enlargement and oedema of the mesenteric lymph nodes, dilatation of lymphatics and gelatinisation of mesenteric fat were observed in goats euthanised at 9 and 12 MPI. Histological lesions consisting of flat and broad villi with mild infiltration of lymphocytes and macrophages in the intestinal villi and crypts were common at 3 MPI and 6 MPI. Focal infiltration of macrophages in Peyer's patches and giant cells were found in the mesenteric lymph nodes at 6 MPI. Broadened villi and increased infiltration of lymphocytes and macrophages with Langhan's giant cells were observed in the villi and crypts at 9 MPI and 12 MPI goats. Acid-fast bacilli were detected in 3 goats, one each at 6, 9 and 12 MPI.

Presence of IgA-producing cells in rats and mice in relation to microbial status

1984 ◽  
Vol 18 (3) ◽  
pp. 252-257 ◽  
Author(s):  
J. W. M. A. Mullink ◽  
F. H. M. Morsink
Keyword(s):  
Lymph Nodes ◽  
Intestinal Mucosa ◽  
Mesenteric Lymph Nodes ◽  
Germ Free ◽  
Mesenteric Lymph ◽  
Significant Difference ◽  
Rats And Mice ◽  
Microbial Status

The numbers of IgA-producing cells in intestinal mucosa, mesenteric lymph nodes, lungs and bronchial lymph nodes were scored in rats and mice. A statistically significant difference in the scores existed between germ-free and SPF mice and between gnotobiotic and SPF rats. In a group of SPF rats a statistically significant difference in the scores was demonstrated in relation to several bacterial and viral agents.

scholarly journals Mesenteric lymph nodes at the center of immune anatomy

2006 ◽  
Vol 203 (3) ◽  
pp. 497-500 ◽  
Author(s):  
Andrew J. Macpherson ◽  
Karen Smith
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Lymph Nodes ◽  
Intestinal Mucosa ◽  
Tolerance Induction ◽  
Intestinal Bacteria ◽  
Mesenteric Lymph Nodes ◽  
Food Proteins ◽  
Mesenteric Lymph ◽  
Food Antigens

The surface of the intestinal mucosa is constantly assaulted by food antigens and enormous numbers of commensal microbes and their products, which are sampled by dendritic cells (DCs). Recent work shows that the mesenteric lymph nodes (MLNs) are the key site for tolerance induction to food proteins and that they also act as a firewall to prevent live commensal intestinal bacteria from penetrating the systemic immune system.

Analysis of T cell populations and IL-3 mRNA expression in mesenteric lymph node cells and intestinal intraepithelial lymphocytes in Strongyloides ratti-infected mice

1998 ◽  
Vol 72 (1) ◽  
pp. 1-8 ◽  
Author(s):  
T. Abe ◽  
H. Sugaya ◽  
K. Yoshimura
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Intraepithelial Lymphocytes ◽  
Cell Populations ◽  
Mrna Levels ◽  
Mesenteric Lymph Nodes ◽  
Intestinal Intraepithelial Lymphocytes ◽  
Mesenteric Lymph ◽  
Lymph Node Cells

AbstractT cell populations and IL-3 mRNA expression were analysed in mesenteric lymph node cells and intestinal intraepithelial lymphocytes (IEL) in Strongyloides ratti- infected mice. On days 7 and 12 post-infection, 2.6 times as many mesenteric lymph node cells were present in S. ratti- infected mice compared with uninfected mice. Although the percentages of CD3+, CD4+ and CD8+ cells decreased during infection, the absolute numbers of these cell types increased on day 7 due to an overall increase in the mesenteric lymph node cell number. The CD4/CD8 ratio in IEL was increased on day 5, whereas no significant change in the CD4/CD8 ratio was observed in the mesenteric lymph node cells. Expression of IL-3 mRNA, which is an important cytokine for the induction of murine mucosal mastocytosis and S. ratti- expulsion, was examined in mesenteric lymph nodes and IEL of uninfected and infected mice. IL-3 mRNA was detected in mesenteric lymph nodes of S. ratti-infected mice but not detected in the lymph nodes of uninfected mice. IL-3 mRNA was detected in IEL from both infected and uninfected mice with an 20-fold increase in expression in IEL of infected mice. Overall, IL-3 mRNA levels were higher in IEL than in mesenteric lymph nodes following S. ratti- infection. Expression of IL-4, IL-10, stem cell factor (SCF or c-kit ligand) and IFN-γ mRNA was also examined in these two tissues. IL-10 mRNA was not detected in any tissue examined and IFN-γ mRNA levels were unaltered as a result of an S. ratti- infection. Elevated expression of mRNA for SCF (5-fold) and IL-4 (20-fold) was observed in the mesenteric lymph nodes of infected mice. In contrast, SCF mRNA levels were similar in IEL of uninfected and infected animals and only a modest increase in IL-4 mRNA was observed in IEL of infected mice.

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