An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants

2006 ◽  
Vol 42 (1) ◽  
pp. 59-64 ◽  
Author(s):  
M. Faisal ◽  
I. Siddique ◽  
M. Anis
Keyword(s):  
Plant Regeneration ◽  
Cotyledonary Node ◽  
Mucuna Pruriens ◽  
Regeneration System ◽  
Cotyledonary Node Explants

scholarly journals In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 318
Author(s):  
Mehtab Muhammad Aslam ◽  
Joseph K. Karanja ◽  
Qian Zhang ◽  
Huifeng Lin ◽  
Tianyu Xia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Genetic Engineering ◽  
Shoot Regeneration ◽  
Lupinus Albus ◽  
Cotyledonary Node ◽  
White Lupin ◽  
Regeneration System ◽  
Regeneration Frequency ◽  
Cotyledonary Nodes

The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

scholarly journals An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

2015 ◽  
Vol 15 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Jyothi Abraham ◽  
T. Dennis Thomas
Keyword(s):  
Age Groups ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Multiple Shoot Induction ◽  
Elephantopus Scaber ◽  
Cotyledonary Node Explants

An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoots per explant. The highest frequency of rooting (100%) and mean number of roots (3.3 per shoot) were observed when the shoots were transferred to MS medium supplemented with 1.0 mg L-1 IBA. The plantlets raised in vitro were acclimatized and transferred to soil with a 92% success rate. The protocol described here may be utilized for multiplication and conservation of elite clones of E. scaber.

A regeneration system using cotyledons and cotyledonary node explants of Toona ciliata

2020 ◽  
Author(s):  
Huiyun Song ◽  
Wenmai Mao ◽  
Yuanyuan Shang ◽  
Wei Zhou ◽  
Pei Li ◽  
...  
Keyword(s):  
Cotyledonary Node ◽  
Regeneration System ◽  
Toona Ciliata ◽  
Cotyledonary Node Explants

scholarly journals Tissue Culture and Plant Regeneration of Cowpea (Vigna unguiculata)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 627f-627
Author(s):  
Lurline Marsh
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Regeneration ◽  
Vigna Unguiculata ◽  
Nutrient Medium ◽  
Cotyledonary Node ◽  
Naphthalene Acetic Acid ◽  
Leaf Segments ◽  
Dichlorophenoxy Acetic Acid ◽  
Cotyledonary Node Explants

Explants (cotyledon, cotyledonary node, second node, hypocotyl, epicotyl, and leaf) of cowpea (Vigna unguiculata) genotypes MN13 and Pinkeye Purple Hull were cultured on Murashige and Skoog basal nutrient medium. The medium was supplemented with 1 mg·L–1 benzyladenine (BA) or 1 mg·L–1 benzyladenine plus naphthalene acetic acid (BA + NAA) or 2 mg·L–1 2,4-dichlorophenoxy acetic acid (2,4-D). Cultures were maintained at 22°C for 1 month, after which they were transferred to 1 mg·L–1 BA + NAA. Cotyledons, hypocotyl, epicotyl, and leaf segments produced only calli after subculturing in BA + NAA. The second node and cotyledonary node explants cultured on the BA or BA + NAA followed by subculture on BA + NAA produced calli, shoots, and roots. The plants were then transplanted to promix but later died.

scholarly journals In vitro Plant Regeneration from Seedling-derived Explants of two Cultivars of White Jute (Corchorus capsularies L.)

2010 ◽  
Vol 19 (2) ◽  
pp. 199-206
Author(s):  
Rakha Hari Sarker ◽  
Bivas Kumar Sarkar ◽  
Bivas Kumar Sarkar ◽  
M. I. Hoque ◽  
M. I. Hoque
Keyword(s):  
Plant Regeneration ◽  
In Vitro Regeneration ◽  
Cotyledonary Node ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Regeneration System ◽  
Shoot Initiation ◽  
Corchorus Capsularis ◽  
In Vitro Plant

An in vitro regeneration system for two varieties of white jute (Corchorus capsularis L.) namely, BJC-7370 and BJC-83 was developed.  The regeneration protocol was based upon direct organogenesis from seedling-derived explants such as cotyledon with petioles, cotyledonary node. Shoot regeneration was achieved from cotyledon with petiole and cotyledonary node through use of MS supplemented with 0.5 mg/l BAP and 1.0 mg/l IAA for BJC-7370 while for BJC-83 shoot initiation was obtained on 1.25 mg/l BAP and 0.25 mg/l NAA using the same explants. Elongation of shoots was achieved on MS containing 0.2 mg/l BAP for the two said jute varieties. Regenerated excised shoots developed effective in vitro root system on half strength of MS supplemented with 0.3 mg/l IBA for both the varieties. The in vitro grown plantlets were transferred to soil for acclimation. These plants grew up to maturity, flowered and produced seeds identical to the control plants.  Key words:  Corchorus capsularies, In vitro plant regeneration, seedling explants D.O.I. 10.3329/ptcb.v19i2.5437 Plant Tissue Cult. & Biotech. 19(2): 199-206, 2009 (December)

scholarly journals Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)

2017 ◽  
Vol 27 (1) ◽  
pp. 41-50
Author(s):  
Nazrul Islam ◽  
Touhidul Islam ◽  
Md Munir Hossain ◽  
Bakul Bhattacharjee ◽  
M Monzur Hossain ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Cotyledonary Node ◽  
Green Plant ◽  
Medium Composition ◽  
Regeneration System ◽  
Embryo Formation ◽  
Half Strength ◽  
Embryogenic Callus Induction

An efficient callus induction and plant regeneration system has been developed using three local soybean (Glycine max L.) varieties. All the varieties showed good callusing (78.30 ‐ 88.80%) from shoot tip (ST) in MS + 3.0 mg/l 2,4‐D + 1.0 mg/l BAP. Best callus induction and embryo formation were recorded in T5 for BS‐6 as 88.80 and 81.20%, respectively from ST. In case of cotyledonary node (CN) the maximum callus induction (82.40%) and embryo formation (74.20%) were recorded also in T5 for BS‐6. Highest frequency (79.40%) of plant regeneration was recorded where ST were used and cultured in MS supplemented with 2.5 mg/l BAP + 1.0 mg/l NAA as well as 76.30% from CN in BS‐6. The length of shoot was observed 5.32 and 4.62 cm, respectively from ST and CN for BS‐6 with the same medium composition. It was observed that half strength of MS + 2.0 mg/l IBA showed best rooting (9.04). Among the genotypes BS‐6 proved to be best explants than ST that exhibited better performance on callus induction and green plant regeneration for all parameters.Plant Tissue Cult. & Biotech. 27(1): 41-50, 2017 (June)

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