scholarly journals The validation of using serum iron increase to measure iron absorption in human subjects

2004 ◽  
Vol 92 (3) ◽  
pp. 485-488 ◽  
Author(s):  
Michael Hoppe ◽  
Lena Hulthén ◽  
Leif Hallberg
Keyword(s):  
Small Dose ◽  
Serum Iron ◽  
Blood Donors ◽  
Iron Absorption ◽  
Human Subjects ◽  
Relative Bioavailability ◽  
Whole Body ◽  
Body Counting ◽  
Whole Body Counting

The objectives of the present study were to study the correlation between the change in serum Fe and Fe absorption when administering 100 mg Fe (as FeSO4) orally, and to study the correlation between the absorption from a 3 mg and a 100 mg Fe (as FeSO4) dose. The study was conducted in a group of eleven male blood donors, without any evident infection, who had given blood 8 weeks before the study. On three consecutive mornings the subjects were served a wheat roll fortified with Fe. On the first 2 d the roll was fortified with 3 mg Fe labelled with 59Fe; on day 3 the roll was fortified with 100 mg Fe labelled with 55Fe. The serum Fe response to the 100 mg dose was followed for 6 h. Fe absorption was measured by whole-body counting. High correlations were seen between the absorption of Fe and the change in serum Fe after 100 mg Fe (r2 0·94, P>0·001), between the absorption from 3 mg and 100 mg Fe (r2 0·88, P>0·001), and between the absorption from 3 mg Fe and change in serum Fe after 100 mg Fe (r2 0·90, P>0·001). This strengthens the evidence that it is possible to use the change in serum Fe as a measure of Fe absorption, e.g. when establishing the relative bioavailability for Fe powders. The results also imply that the induced serum Fe increase following 100 mg Fe added to a food could predict the Fe absorption of a small dose of Fe added to the same meal.

scholarly journals Brief Note: A Note Concerning the "100 Per Cent Value" in Iron Absorption Studies by Whole Body Counting

Blood ◽  
1964 ◽  
Vol 23 (6) ◽  
pp. 757-761 ◽  
Author(s):  
LEWIS M. SCHIFFER ◽  
D. C. PRICE ◽  
J. CUTTNER ◽  
S. H. COHN ◽  
EUGENE P. CRONKITE
Keyword(s):  
Iron Absorption ◽  
Whole Body ◽  
Body Counter ◽  
Whole Body Counter ◽  
Absorption Studies ◽  
Body Counting ◽  
Whole Body Counting ◽  
Body Count

Abstract The 4-hour whole body count is found to be clinically valid as a "100 per cent value" in iron absorption studies performed with a whole body counter. Measurement of iron absorption can be made 2 weeks after ingestion of radioiron, but not prior to this period.

A Preliminary Study of Iron Absorption by Whole Body Counting and Correlation with DFO Excretion

1975 ◽  
Vol 14 (01) ◽  
pp. 74-80
Author(s):  
M. M. Gupta ◽  
R. Manchanda ◽  
L. Bhattacharyya ◽  
M. Bhargva ◽  
S. Kumar ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anaemia ◽  
Iron Absorption ◽  
Whole Body ◽  
Healthy Controls ◽  
Deficiency Anaemia ◽  
Iron Deficient ◽  
Body Counting ◽  
Preliminary Study ◽  
Whole Body Counting

SummaryA preliminary study of iron absorption by whole body counting was carried on a group of 16 women. The cases included 8 patients suffering from iron deficiency anaemia and various infections as well as 8 healthy controls. High iron absorption is associated with iron dificiency, these changes being more marked in iron deficient controls than in those with infection or malignancy. In iron deficient controls results of whole body counting correlate very well with other haematological investigations.

36. Iron absorption from a natural mineral water by whole-body counting

1996 ◽  
Vol 17 (11) ◽  
pp. 995
Author(s):  
R. J. Villis ◽  
W. D. Evans ◽  
M. Worwood ◽  
A. K. Burnett
Keyword(s):  
Mineral Water ◽  
Iron Absorption ◽  
Whole Body ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Body Counting ◽  
Whole Body Counting

scholarly journals An animal model to study iron availability from human diets

1977 ◽  
Vol 37 (3) ◽  
pp. 451-456 ◽  
Author(s):  
B. S. Narasinga Rao ◽  
J. Siva Prasad ◽  
C. Vijaya Sarathy
Keyword(s):  
Animal Model ◽  
Guinea Pigs ◽  
Ferrous Iron ◽  
Human Subjects ◽  
Whole Body ◽  
Iron Availability ◽  
Retention Ratio ◽  
Ferrous Ascorbate ◽  
Body Counting ◽  
Whole Body Counting

1. The retention of ferric- and ferrous-iron was determined in guinea-pigs and monkeys using55Fe and59Fe.2. The bioavailability of Fe from two typical Indian diets based on rice and wheat was determined in humans and monkeys using a59Fe tracer and whole-body counting.3. The retention ratio, ferric-Fe; ferrous-Fe was 0.90 in guinea-pigs and 0.33 in monkeys, indicating that monkeys absorb ferrous-Fe preferentially.4. In monkeys retention of Fe from the test diets, as from ferrous ascorbate was lower than that in humans.5. When food-Fe retention was expressed in relation to inorganic-Fe retention the value for retention ratio, food Fe: inorganic Fe in monkeys was similar to that in human subjects.6. The results indicate that the monkey can be used as a model to study Fe absorption from human diets.

scholarly journals The Gastrointestinal Tract and Iron Absorption

Blood ◽  
1963 ◽  
Vol 22 (4) ◽  
pp. 416-428 ◽  
Author(s):  
MUNSEY S. WHEBY ◽  
WILLIAM H. CROSBY ◽  
Betty Merrill ◽  
Natalie Lawson
Keyword(s):  
Iron Absorption ◽  
Initial Period ◽  
Whole Body ◽  
Gradual Loss ◽  
Iron Deficient ◽  
Body Counting ◽  
Two Phases ◽  
Whole Body Counting ◽  
The Relationship ◽  
Mucosal Uptake

Abstract 1. Using Fe59 and whole body counting, various aspects of gastrointestinal absorption of iron salts were studied in normal, iron-loaded, and iron-deficient rats. 2. Following a single intragastric dose of iron, peak small intestinal uptake was observed by 30 to 60 minutes with subsequent gradual loss over 24-hour period. 3. Iron absorption showed two phases, an initial period of rapid absorption lasting up to 2 hours during which 60 to 80 per cent of total absorption into carcass took place. The remainder took place at a slow rate over the subsequent 12 to 20 hours. 4. Depending on the relationship between body iron stores and dose of administered iron, the intestine may take up more iron than is ultimately transferred to plasma. A variable amount of this iron is lost when the epithelial cell is sloughed into the gastrointestinal lumen. 5. Regulation of iron absorption appears to involve two steps, mucosal uptake and transfer to the blood.

scholarly journals The Determination of Iron Absorption and Loss by Whole Body Counting

Blood ◽  
1962 ◽  
Vol 20 (5) ◽  
pp. 517-531 ◽  
Author(s):  
D. C. PRICE ◽  
S. H. COHN ◽  
L. R. WASSERMAN ◽  
P. G. REIZENSTEIN ◽  
EUGENE P. CRONKITE
Keyword(s):  
Iron Deficiency ◽  
Aplastic Anemia ◽  
Iron Absorption ◽  
Whole Body ◽  
Whole Body Counter ◽  
History Of ◽  
Body Counting ◽  
Menstruating Women ◽  
Whole Body Counting

Abstract A technic for the study of radioiron absorption and loss is described employing an NaI (T1) crystal-detector whole body counter and 1-10 µc. Fe59 in 250 µg. elemental iron. Changes in whole body Fe59 activity during the first few hours and the next 90-100 days after oral ingestion are described and their significance discussed. Normal absorption with this technic ranges from 5.7-24.7 per cent of the administered tracer. In 14 patients with polycythemia vera, 12 previously phlebotomized and 2 with a recent history of gastrointestinal hemorrhage, iron deficiency as evidenced by increased iron absorption (20.6 per cent-96.9 per cent) correlates well with the extent of preceding phlebotomy, and relatively well with the plasma iron at the time of study. Although other parameters reflect iron deficiency, none correlate well with the absorption of radioiron. Next to increased iron absorption, depletion of iron stores in the marrow seems to be the earliest evidence of iron deficiency. Iron absorption and erythrocyte incorporation of radioiron was also studied in several other hematologic disorders, including four heavily menstruating women, three cases of aplastic anemia, and a small number of other conditions. The findings are described and discussed. Radioiron loss in three normal patients was 0.110 per cent, 0.110 per cent, and 0.182 per cent daily, and in two patients with aplastic anemia 0.103 per cent and 0.173 per cent daily, defining the normal range of tracer loss over days 20-100. Radioiron loss in the polycythemics ranged from 0-0.044 per cent daily. An unusual case of pyridoxine-responsive anemia with increased absorption of radioiron (69.1 per cent), but no red cell incorporation, lost only 0.026 per cent/day. Some problems in the interpretation of such data are discussed. The results demonstrate the effectiveness of the technic of whole body counting in the study of various aspects of iron metabolism.

Comparison of Stable Isotopes and Radioisotopes in the Measurement of Iron Absorption in Healthy Women

1994 ◽  
Vol 87 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Jon F. R. Barrett ◽  
Paul G Whittaker ◽  
John D. Fenwick ◽  
John G. Williams ◽  
Tom Lind
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotopes ◽  
Inductively Coupled Plasma ◽  
Iron Absorption ◽  
Whole Body ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Body Counting ◽  
The Mean ◽  
Whole Body Counting

1. Stable isotope methods are being used to investigate the absorption of dietary iron. In order to be certain that this new methodology is accurate, we have compared results obtained using stable isotopes and inductively coupled plasma mass spectrometry with those determined using a radioisotope and whole body counting. 2. The stable isotope 54Fe (2.8 mg) was given to 10 healthy non-pregnant women. Six women received the isotope in aqueous form, and four took it with a meat meal. The 54Fe served as a carrier for 10 ng of the radioisotope 59Fe. An ampoule (200 μg) of the isotope 57Fe or 58Fe was then given intravenously, and in serum samples taken over the next 10 h the ratios of the stable iron isotopes were measured by inductively coupled plasma mass spectrometry and the oral iron absorption was calculated. This was then compared with the results obtained by using a whole body counter to measure (on day 0 and day 14) the γ-activity emitted by the radioisotope. 3. The mean iron absorption measured by both methods ranged from 8% to 45%. Measurement of the post-absorptive serum enrichment of the stable isotopes provided estimates of absorption from both aqueous and food iron which were similar to that yielded by whole body counting, the mean difference being −1.5% (95% confidence interval −5.2 to 2.1%). Absorption estimated by stable isotopes exhibited the same inverse relationship with the serum ferritin level (body iron stores) to that known to exist with whole body counting. Similar estimates of food iron absorption were obtained irrespective of the type of isotope used as an extrinsic label, implying that stable isotopes are as valid as radioisotopes in reflecting intrinsic food iron absorption. 4. This study validates the use of stable isotopes and post-absorption curves as a new and accurate technique in the measurement of iron absorption.

A Simple Method for Measuring Iron Absorption Using a Scintiscanner for Whole-body Counting

1967 ◽  
Vol 40 (479) ◽  
pp. 866-868 ◽  
Author(s):  
G. M. Owen ◽  
S. Kirkman ◽  
M. Williams ◽  
A. Jacobs
Keyword(s):  
Iron Absorption ◽  
Whole Body ◽  
Simple Method ◽  
Body Counting ◽  
Whole Body Counting
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