scholarly journals Carbohydrates digestibility and faecal nitrogen excretion in rats fed raw or germinated faba bean (Vicia faba)- and chickpea (Cicer arietinum)-based diets

2003 ◽  
Vol 90 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Luis A. Rubio

Raw or germinated faba bean (Vicia fabaminor var. Alameda) and chickpea(Cicer arietinumkabuli var. Athenas) seed meals were incorporated in essential amino acid-supplemented and energy-equalized diets for growing (65 (SD 1) g) male Wistar rats as the only sources of dietary protein. A lactalbumin-based diet was used as the control. Faecal dry weight and N excretion of animals fed legume-containing diets were greater (P<0·01) and DM digestibility lower (P<0·01) than controls. Apparent faecal digestibilities of amino acids were found to be not different or lower (P<0·01) than controls in rats fed diets containing legume seeds, either germinated or not. Total diaminopimelic acid (DAPA) and purine bases excretion were significantly (P<0·01) higher than controls in rats fed both legume seed meals. Faecal bacterial N calculated according to DAPA or purine bases values was similar and significantly higher (P<0·01) than controls in rats fed legume seed meals. Bacterial N accounted for 50 to 80 % of total faecal N in rats fed legume diets. Apparent faecal N digestibility values (53–65 %) of rats fed legume-based diets were lower (P<0·01) than controls, but became substantially higher (85–92 %) when calculated taking into account bacterial N values. Faecal NSP digestibility values for legume diets were 40–57 g/100 g ingested. Germination decreased starch faecal excretion (P<0·05) and increased (P<0·05) faecal starch and NSP digestibilities of faba bean seeds.

Author(s):  

Abstract A new distribution map is provided for Chickpea chlorotic dwarf virus Virus: Geminiviridae: Mastrevirus Hosts: Chickpea (Cicer arietinum) and faba bean (Vicia faba). Information is given on the geographical distribution in ASIA, India, Himachal Pradesh, Madhya Pradesh, Iran, Iraq, Pakistan, Syria, Yemen, AFRICA, Egypt, Ethiopia, Sudan.


2002 ◽  
Vol 82 (14) ◽  
pp. 1710-1717 ◽  
Author(s):  
Luis A Rubio ◽  
Mercedes Muzquiz ◽  
Carmen Burbano ◽  
Carmen Cuadrado ◽  
Mercedes M Pedrosa

1992 ◽  
Vol 67 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Luis A. Rubio ◽  
George Grant ◽  
Susan Bardocz ◽  
Peter Dewey ◽  
A. Pusztai

The effects on faecal mineral excretion of two commercial varieties (local cultivar and Troy cultivar) of raw faba beans (Vicia faba L., minor) meal (VFM) and its fractions have been studied in growing rats. Diets contained local-VFM (dark seed coat) and Troy-VFM (light seed coat) at 474–500 g/kg diet, hull (VFH) from both varieties at 65 g/kg diet, and the insoluble cotyledon residue (VFCR) obtained from the Troy variety at 237 g/kg diet. Rats were pair-fed on diets which had been supplemented with amino acids to target requirements and contained similar amounts of zinc, manganese, iron and copper. With VFM diets the apparent absorption of Zn and Mn was significantly reduced. On the other hand, with hulls the apparent absorption of Fe was reduced while that of Cu slightly increased. As the amounts of calcium, phosphorus and magnesium in VFM and VFH diets were higher than in the controls, the increased intake resulted in a significant increase in both the apparent absorption and the faecal excretion of these minerals. The inclusion of VFCR in the diet had no significant effect on the mineral content of faeces. The relatively low concentrations of phytate in the bean seeds of 7.8 and 6.7 g/kg for the local and Troy cultivars respectively, could not adequately account for the increased mineral excretion. The results suggest that other seed constituents, possibly the soluble non-starch polysaccharides, may be involved in the elevated loss of Zn and Mn in rats fed on diets containing faba bean for extended periods, while some insoluble structural hull components may interfere with the absorption of Fe from the gut.Faba bean (Vicia faba): Faecal mineral excretion: Rat.


Author(s):  

Abstract A new distribution map is provided for Chickpea chlorotic dwarf virus. Geminiviridae: Mastrevirus. Hosts: chickpea (Cicer arietinum), faba bean (Vicia faba). Information is given on the geographical distribution in Asia (India, Himachal Pradesh, Madhya Pradesh, Rajasthan, Iran, Iraq, Oman, Pakistan, Syria, Turkey, Yemen), Africa (Burkina Faso, Egypt, Eritrea, Ethiopia, Morocco, Nigeria, South Africa, Sudan, Tunisia).


2001 ◽  
Vol 85 (5) ◽  
pp. 607-614 ◽  
Author(s):  
M. Teresa Macarulla ◽  
César Medina ◽  
M. Aránzazu De Diego ◽  
M. Chávarri ◽  
M. Ángeles Zulet ◽  
...  

The aim of the present work was to analyse the hypocholesterolaemic efficiency of aVicia faba-protein isolate in relation to the intact legume. In addition, the mechanisms underlying the effects of this isolate were investigated. Hypercholesterolaemic rats were divided into three groups (n 10×3) and fed high-fat diets rich in cholesterol-containing casein, whole seeds ofVicia fabaor the protein isolate of faba beans as protein source, for 2 weeksad libitum. The protein isolate was prepared by isoelectric precipitation and spray dried. Analyses of serum, liver and faeces, as well as of the activity of hepatic 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase, were assessed by enzymatic methods. The rats fed onVicia fabadiets showed significantly lower body weights and energy intakes than rats fed on casein diets. The whole-seed diet induced a significant reduction in plasma triacylglycerol. Feeding rats on diets containing faba bean seeds, or the protein isolate, induced a significant decrease in plasma (LDL+VLDL)-cholesterol but not in HDL-cholesterol. Hepatic cholesterol and triacylglycerol were also reduced. The hypocholesterolaemic effects ofVicia fabawere not the result of a reduction in cholesterol synthesis as assessed from HMG-CoA reductase activity, but the result of an increase in steroid faecal excretion. The faba bean-protein isolate obtained under our experimental conditions was useful in improving the metabolic alterations induced by feeding with a hypercholesterolaemic diet compared with casein. The effectiveness of the whole seeds was higher than that of the protein isolate.


2010 ◽  
Vol 337 (1-2) ◽  
pp. 425-434 ◽  
Author(s):  
Francisco J. López-Bellido ◽  
Rafael J. López-Bellido ◽  
Ramón Redondo ◽  
Luis López-Bellido

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1227-1237 ◽  
Author(s):  
C. Palomino ◽  
Z. Satovic ◽  
J.I. Cubero ◽  
A.M. Torres

A PCR approach with degenerate primers designed from conserved NBS–LRR (nucleotide binding site – leucine-rich repeat) regions of known disease-resistance (R) genes was used to amplify and clone homologous sequences from 5 faba bean (Vicia faba) lines and 2 chickpea (Cicer arietinum) accessions. Sixty-nine sequenced clones showed homologies to various R genes deposited in the GenBank database. The presence of internal kinase-2 and kinase-3a motifs in all the sequences isolated confirm that these clones correspond to NBS-containing genes. Using an amino-acid sequence identitiy of 70% as a threshold value, the clones were grouped into 10 classes of resistance-gene analogs (RGA01 to RGA10). The number of clones per class varied from 1 to 30. RGA classes 1, 6, 8, and 9 were comprised solely of clones isolated from faba bean, whereas classes 2, 3, 4, 5, and 7 included only chickpea clones. RGA10, showing a within-class identity of 99%, was the only class consisting of both faba bean and chickpea clones. A phylogenetic tree, based on the deduced amino-acid sequences of 12 representative clones from the 10 RGA classes and the NBS domains of 6 known R genes (I2 and Prf from tomato, RPP13 from Arabidopsis, Gro1–4 from potato, N from tobacco, L6 from flax), clearly indicated the separation between TIR (Toll/interleukin-1 receptor homology: Gro1–4, L6, N, RGA05 to RGA10)- and non-TIR (I2, Prf, RPP13, RGA01 to RGA04)-type NBS–LRR sequences. The development of suitable polymorphic markers based on cloned RGA sequences to be used in genetic mapping will facilitate the assessment of their potential linkage relationships with disease-resistance genes in faba bean and chickpea. This work is the first to report on faba bean RGAs.


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