scholarly journals Dephytinisation of soyabean protein isolate with low native phytic acid content has limited impact on mineral and trace element absorption in healthy infants

2004 ◽  
Vol 91 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Lena Davidsson ◽  
Ekhard E. Ziegler ◽  
Peter Kastenmayer ◽  
Peter van Dael ◽  
Denis Barclay
Keyword(s):  
Stable Isotope ◽  
Trace Element ◽  
Phytic Acid ◽  
Protein Isolate ◽  
Soya Protein ◽  
Faecal Excretion ◽  
Phytic Acid Content ◽  
Healthy Infants ◽  
Isotope Technique ◽  
Stable Isotope Technique

Infant formulas based on soyabean protein isolate are often used as an alternative to cows'-based formulas. However, the presence of phytic acid in soya formulas has raised concern about the absorption of trace elements and minerals from these products. The aim of the present study was to evaluate mineral and trace element absorption from regular and dephytinised soya formula in healthy infants. Soyabean protein isolate with a relatively low native content of phytic acid was used for production of a regular soya formula (300 mg phytic acid/kg liquid formula) and an experimental formula was based on dephytinised soya protein isolate (<6 mg phytic acid/kg liquid formula). Using a crossover study design, apparent mineral and trace element absorptions were measured by a stable isotope technique based on 72 h faecal excretion of non-absorbed stable isotopes (Zn, Fe, Cu and Ca) and by the chemical balance technique (Mn, Zn, Cu and Ca) in nine infants (69–191 d old). Fe absorption was also measured by erythrocyte incorporation 14 d after intake. The results from the present study demonstrated that Zn absorption, measured by a stable isotope technique, was significantly greater after dephytinisation (mean value 16·7 v. 22·6 %; P=0·03). No other statistically significant differences between the two formulas were observed. The nutritional benefit of dephytinisation was marginal in the present study. Based on these results, the use of soyabean protein isolate with low native content of phytic acid should be promoted for production of soya formulas and adequate addition of ascorbic acid to enhance Fe absorption should be ensured in the products.

scholarly journals Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid

2001 ◽  
Vol 85 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Lena Davidsson ◽  
Triantafillia Dimitriou ◽  
Thomas Walczyk ◽  
Richard F. Hurrell
Keyword(s):  
Ascorbic Acid ◽  
Stable Isotope ◽  
Pisum Sativum ◽  
Phytic Acid ◽  
Infant Formula ◽  
Geometric Mean ◽  
Protein Isolate ◽  
Infant Formulas ◽  
Phytic Acid Content ◽  
Pea Protein

Infant formula based on pea (Pisum sativum)-protein isolate has been suggested as an alternative to soyabean formula in countries where soyabean is not a native crop, or when soyabean protein cannot be used due to allergic reactions or intolerances. In the present study, Fe absorption from experimental infant formulas based on pea-protein isolate was measured in healthy non-anaemic young women. The influence of phytic acid and ascorbic acid on Fe absorption was evaluated, using a stable-isotope technique based on incorporation of Fe stable-isotope labels into erythrocytes 14 d after administration. Geometric mean Fe absorption increased from 20·7 (+1SD 41·6, -1sd 10·3) % to 33·1 (+1sd 58·6, -1sd 18·7) %; (P<0·0001; n 10) after enzymic degradation of virtually all phytic acid. Doubling the molar ratio Fe : ascorbic acid from 1 : 2·1 to 1 : 4·2 in the infant formula with native phytic acid content also increased Fe absorption significantly (P<0·0001; n 10); geometric mean Fe absorption increased from 14·8 (+1sd 32·1, -1sd 6·8) % to 22·1 (+1sd 47·2, -1sd 10·4) %. These results confirm the inhibitory and enhancing effects of phytic acid and ascorbic acid respectively on Fe absorption, but also indicate relatively high fractional Fe absorption from the pea-protein-based formulas. After adjusting for differences in Fe status, our data indicate that Fe absorption from dephytinised pea protein might be less inhibitory than dephytinised soyabean protein as measured in a previous study ().

scholarly journals A double stable isotope technique for measuring iron absorption in infants

1994 ◽  
Vol 71 (3) ◽  
pp. 411-424 ◽  
Author(s):  
Peter Kastenmayer ◽  
Lena Davidsson ◽  
Pilar Galanz ◽  
Françise Cherouvrier ◽  
Serge Hercberg ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Thermal Ionization Mass Spectrometry ◽  
Geometric Mean ◽  
Random Order ◽  
Dietary Factors ◽  
Ionization Mass ◽  
Fe Content ◽  
Healthy Infants ◽  
Isotope Technique ◽  
Stable Isotope Technique

A stable isotope technique has been developed which uses 57Fe and 58Fe as labels and which enables the simultaneous measurement of Fe absorption from two test meals in infants. The method was evaluated by measuring Fe absorption from a commercial whey-adjusted infant formula in nine healthy infants aged 13–25 weeks. Each infant was fed 210 ml formula, labelled with either 57Fe or 58Fe, on four consecutive mornings, in random order. The total Fe content in each feed was 2.5 mg Fe; either as 2.5 mg 57Fe, or 0 6 mg 58Fe plus 1.9 mg Fe with normal isotopic composition. Isotopic enrichment of Fe in erythrocytes was measured by thermal ionization mass spectrometry 14 d after the last administration, and Fe absorption was calculated based on isotope ratio shifts, total circulating Fe and intake of each isotope. Geometric mean absorption for the 57Fe and 58Fe labels was 6.72 and 658% respectively, and the absorption of the two isotopes was not significantly different (Student's paired t test). By this technique, paired comparisons of Fe absorption can be obtained and systematic studies of the influence of dietary factors on Fe absorption during infancy can he conducted.

Conventional and isotopic determinations of shorebird diets at an inland stopover: the importance of invertebrates and Potamogeton pectinatus tubers

1996 ◽  
Vol 74 (6) ◽  
pp. 1057-1068 ◽  
Author(s):  
Stuart A. Alexander ◽  
Keith A. Hobson ◽  
Cheri L. Gratto-Trevor ◽  
Antony W. Diamond
Keyword(s):  
Stable Isotope ◽  
Potamogeton Pectinatus ◽  
Gut Content Analysis ◽  
Gut Content ◽  
Geographic Ranges ◽  
Isotope Technique ◽  
Wetland Complex ◽  
Limnodromus Scolopaceus ◽  
Stable Isotope Technique

We used gut-content and stable-isotope techniques to determine diets of shorebirds staging at a prairie wetland complex. Stable-isotope ratios for carbon (13C/12C) and nitrogen (15N/14N) varied greatly within and among prey types and shorebirds, depending on location within the complex. Both dietary techniques suggested that Long-billed Dowitchers (Limnodromus scolopaceus) and Stilt Sandpipers (Calidris himantopus) ate mostly invertebrates, whereas Hudsonian (Limosa haemastica) and Marbled godwits (Limosa fedoa) ate mainly Potamogeton pectinatus tubers. In comparison, the stable-isotope technique indicated that godwits, especially juvenile Marbled Godwits, ate more invertebrates than is indicated by the gut-content analysis. The discrepancies between methods reflect the potential for bias in the application of these techniques. Researchers using stable isotopes to assess migratory shorebird diets should be aware of possible complications arising from isotopic variability within prey types, even over small geographic ranges. High isotopic variability at inland agro-wetland complexes might preclude reliable isotopic assessment of shorebird diets, especially long term. Rhizivory in godwits may be more common than is generally recognized, especially at inland stopovers during autumn migration in both North America and Eurasia, and should be factored into conservation initiatives for these species.

The relationship between shrimp (Litopenaeus vannamei) size and biofloc consumption determined by the stable isotope technique

Aquaculture ◽  
2020 ◽  
Vol 529 ◽  
pp. 735635
Author(s):  
Dariano Krummenauer ◽  
Paulo C. Abreu ◽  
Luiz Poersch ◽  
Paula Alice Cruz Paiva Reis ◽  
Sabrina Medeiros Suita ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Litopenaeus Vannamei ◽  
Isotope Technique ◽  
The Relationship ◽  
Stable Isotope Technique

Using enriched stable isotope technique to study Cu bioaccumulation and bioavailability in Corbicula fluminea from Taihu Lake, China

2014 ◽  
Vol 21 (24) ◽  
pp. 14069-14077 ◽  
Author(s):  
Wenhong Fan ◽  
Jinqian Ren ◽  
Chenguang Wu ◽  
Cheng Tan ◽  
Xiaolong Wang ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Taihu Lake ◽  
Corbicula Fluminea ◽  
Isotope Technique ◽  
Stable Isotope Technique

Using stable isotope technique to study feeding habits of the finless porpoise (Neophocaena asiaeorientalisssp.sunameri)

2016 ◽  
Vol 36 (1) ◽  
Author(s):  
鹿志创 LU Zhichuang ◽  
田甲申 TIAN Jiashen ◽  
王召会 WANG Zhaohui ◽  
马志强 MA Zhiqiang ◽  
韩家波 HAN Jiabo ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Feeding Habits ◽  
Finless Porpoise ◽  
Isotope Technique ◽  
Stable Isotope Technique

Tracing the major source area of the mountainous runoff generation of the Heihe River in northwest China using stable isotope technique

2009 ◽  
Vol 54 (16) ◽  
pp. 2751-2757 ◽  
Author(s):  
NingLian Wang ◽  
ShiBiao Zhang ◽  
JianQiao He ◽  
JianChen Pu ◽  
XiaoBo Wu ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Source Area ◽  
Northwest China ◽  
Heihe River ◽  
Runoff Generation ◽  
Isotope Technique ◽  
Stable Isotope Technique
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