scholarly journals Hepatic protein synthesis in the sheep: effect of intake as by use of stable-isotope-labelled glycine, leucine and phenylalanine

1997 ◽  
Vol 77 (2) ◽  
pp. 255-271 ◽  
Author(s):  
A. Connell ◽  
A. G Calder ◽  
S. E Anderson ◽  
G. E Lobley*
Keyword(s):  
Protein Synthesis ◽  
Plasma Protein ◽  
Liver Protein ◽  
Plasma Samples ◽  
Total Plasma ◽  
Plasma Albumin ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Apolipoprotein B100 ◽  
Hepatic Portal

Rates of protein synthesis for the liver, plasma albumin and total plasma protein were quantified in sheep either offered a supra-maintenance intake or fasted for 3 d. The technique of continuous infusion over a 12 h period was employed with the simultaneous infusion of [1-13C]glycine, [1-13C]leucine and [2H5]phenylalanine. Blood and plasma samples were removed at timed intervals from the hepatic portal and hepatic veins plus the aorta. Enrichments of the free amino acids (AA) were determined in all blood and plasma samples as was the protein-bound AA in an apolipoprotein B100 extract. Protein-bound phenylalanine enrichments were also measured in albumin and total protein from plasma plus samples from liver biopsies. The apolipoprotein B100 enrichments agreed well with those of the free AA in hepatic (and hepatic portal) plasma but were lower than for arterial free AA and greater than liver homogenate free AA. This adds support to the concept that export proteins may preferentially use AA directly from extracellular sources. Intake had no significant effect on constitutive liver protein synthesis and the values agreed well with those obtained by other isotopic approaches. There were, however, signicant declines, based on hepatic venous free phenylalanine enrichment, at the lower intake in both the fractional (3·4v. 4·7 % per d;P=0·024) and absolute (2·4v. 4·2 g/d;P=0·011) synthesis rates of albumin, which matched the estimated decrease in total plasma albumin content (52v. 67 g,P<0·01). In contrast, there was a smaller reduction in total plasma protein mass (145v. 151 g,P=0·035) with no observed significant difference in kinetic parameters. Albumin synthesis was calculated to account for a maximum of 17 % of total liver protein synthesis in the fed condition and this may fall to 8 % during moderate fasts.

PSIX-13 Activation of cell signaling pathways by galectins in cow blood

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 267-267
Author(s):  
Djaafar M Rehrah ◽  
Mulumebet Worku ◽  
Hamid Ismail
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
Plasma Protein ◽  
Group Treatment ◽  
Control Group ◽  
Antibody Array ◽  
Total Plasma ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Cell Signaling Pathways

Abstract Galectins are part of a conserved family of β-galactoside-binding proteins that contribute to critical biological events during mammalian gestation and increasingly recognized for a possible role in the immune response of the cow. The objective of this study was to evaluate the effect of Galectins on signal transduction and cell activation in cow blood. Whole blood was collected aseptically from the jugular vein of healthy Holstein Friesian dairy cows (N=3). Blood samples (2.5ml) in duplicate were treated with 150µl of the four different type of recombinant galectins (1, 3, 4, and 9) respectively and untreated samples were served as control. The concentration of total plasma protein was determined using the Pierce BCA kit. Protein expression profiling was performed using1,358 antibodies on the Full Moon BioSystems’ Signaling Explorer antibody array covering 20 cell signaling pathways, as recommended by the manufacturer using an Agilent microarray scanner. Data normalization was performed using GeneSpring GX software to generate fold changes in gene expression and then filtered to obtain a list of significantly upregulated and downregulated genes. Features were extracted from protein array images of samples treated with Galectin 1, 3, 4, 9, and untreated sample as a control group. Treatment with all four Galectins increased the concentration of total plasma protein. Average increases due to treatment with Gal1, Gal3, Gal4, and Gal9 were 27%, 10%, 20%, and 14% respectively. ANOVA test showed significance difference among the groups (p &lt; 0.05). Dunnett option was used to compare each of the treated samples to the control group as a baseline. The results also showed that there was significant difference between the control group and any of the treated group (p &lt; 0.05). Distinct signaling pathways are activated in response to Galectin exposure. Further studies are needed to define their regulation and functional impact on cow health.

Effects of Electroacupuncture on Leukocytes and Plasma Protein in the X-irradiated Rats

1984 ◽  
Vol 12 (01n04) ◽  
pp. 106-114 ◽  
Author(s):  
D.M. Hau
Keyword(s):  
Plasma Protein ◽  
Inhibitory Effect ◽  
Total Plasma ◽  
Plasma Albumin ◽  
X Ray ◽  
Total Plasma Protein ◽  
X Irradiation

The effects of electroacupunture on leukocytes and plasma protein on X ray-irradiated rats were investigated in the present study. The results showed that X-irradiation had an evident inhibitory effect on the counts of total leukocytes, lymphocytes and neutrocytes, and the concentration of the total plasma protein, plasma albumin, globulin and α- and β-globulin in X-irradiated rats. The electroacupuncture was able to help the X-irradiated rats to recover the counts of the total leukocyte, lymphocyte and neutrocyte. The electroacupuncture had a helpful tendency to recover the concentration of the total plasma protein, albumin, globulin, and α- and β-globulin in the irradiated rats.

scholarly journals Hematological profile, total plasma protein and fibrinogen concentrations of clinically healthy adult Campeiro horses

2015 ◽  
Vol 46 (1) ◽  
pp. 144-149 ◽  
Author(s):  
Joandes Henrique Fonteque ◽  
Marcio Luiz Ceccatto ◽  
Renan Menegasso Bagio ◽  
Jackson Schade ◽  
Mere Erika Saito ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Healthy Adult ◽  
Complete Blood Count ◽  
Age Groups ◽  
Total Plasma ◽  
Blood Profile ◽  
Pregnant Females ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Student’S T

ABSTRACT: This research was performed by a multidisciplinary and interagency team and sought to determine the blood profile, total plasma protein and fibrinogen concentrations of clinically healthy adult Campeiro horses. A total of 138 horses (14 stallions, 74 non-pregnant and 50 pregnant mares) over three years of age from breeders located in the states of Santa Catarina and Rio Grande do Sul were divided into groups according to age, sex and pregnancy status. Statistical analysis of the data was performed using the ANOVA test, Student's T test, and descriptive analyses (P<0.05). There was a significant difference (P<0.05) in the values for MCV, MCH, and total number of eosinophiles when comparing different age groups. There was also a notable difference (P<0.05) in the total plasma protein and total number of eosinophile variables when comparing pregnant females to non-pregnant females. These results show that the Campeiro breed presents certain peculiarities regarding variables in the complete blood count, total plasma protein and fibrinogen compared to the values described by other authors for other breeds. Thus, it is suggested that the values established in this study should be used as benchmarks for interpreting erythrocyte and leukocyte counts when evaluating these variables in Campeiro horses.

Changes in the concentration of plasma proteins in intensively bred ewes

1977 ◽  
Vol 88 (2) ◽  
pp. 283-288 ◽  
Author(s):  
W. S. Mackie
Keyword(s):  
Plasma Protein ◽  
Plasma Volume ◽  
Plasma Proteins ◽  
Gamma Globulin ◽  
Total Plasma ◽  
Plasma Albumin ◽  
Total Plasma Protein ◽  
The Mean ◽  
Mean Concentration ◽  
Intensive Breeding

SUMMARYThe mean concentration of plasma albumin in 30 Finnish Landrace x Dorset Horn ewes subjected to intensive breeding decreased as the number of pregnancies increased from three to seven; it also changed significantly within a pregnancy. Gamma globulin concentration was not affected by the number of pregnancies but decreased significantly within a pregnancy. Total plasma protein was affected like albumin by the number and stage of pregnancy.Plasma volume increased by 23 % in the latter third of pregnancy.

scholarly journals Blood parasites, total plasma protein and packed cell volume of small wild mammals trapped in three mountain ranges of the Atlantic Forest in Southeastern Brazil

2007 ◽  
Vol 67 (3) ◽  
pp. 531-535 ◽  
Author(s):  
MAML. Silva ◽  
A. Ronconi ◽  
N. Cordeiro ◽  
DEP. Bossi ◽  
HG. Bergallo ◽  
...  
Keyword(s):  
Cell Volume ◽  
Atlantic Forest ◽  
Plasma Protein ◽  
Packed Cell Volume ◽  
Blood Parasites ◽  
Southeastern Brazil ◽  
Total Plasma ◽  
Wild Mammals ◽  
Total Plasma Protein ◽  
Significant Difference

A study of blood parasites in small wild non-flying mammals was undertaken in three areas of the Atlantic Forest in Southeastern Brazil: Serra de Itatiaia, RJ, Serra da Bocaina, SP and Serra da Fartura, SP, from June 1999 to May 2001. A total of 450 animals (15 species) were captured in traps and it was observed in 15.5% of the blood smears the presence of Haemobartonella sp. and Babesia sp. in red blood cells. There was no statistically significant difference between parasited and non-parasited specimens regarding total plasma protein, packed cell volume and body weight, which strongly suggests that these specimens might be parasite reservoirs.

THE EFFECT OF AGE AND THE PLANE OF NUTRITION ON THE BLOOD CHEMISTRY OF THE COLUMBIAN BLACK-TAILED DEER (ODOCOILEUS HEMIONUS COLUMBIANUS): B. BLOOD GLUCOSE, NON-PROTEIN NITROGEN, TOTAL PLASMA PROTEIN, PLASMA ALBUMIN, GLOBULIN, AND FIBRINOGEN

1957 ◽  
Vol 35 (2) ◽  
pp. 283-289 ◽  
Author(s):  
P. J. Bandy ◽  
W. D. Kitts ◽  
A. J. Wood ◽  
I. McT. Cowan
Keyword(s):  
Blood Sugar ◽  
Plasma Protein ◽  
Odocoileus Hemionus ◽  
Blood Chemistry ◽  
Total Plasma ◽  
Odocoileus Hemionus Columbianus ◽  
Plasma Albumin ◽  
Protein Nitrogen ◽  
Blood Constituents ◽  
Total Plasma Protein

The blood sugar, non-protein nitrogen, total plasma protein, plasma albumin, globulin, and fibrinogen of the Columbian black-tailed deer have been studied on two series of animals reared on high and low planes of nutrition from birth to 465 days of age. Of these blood constituents only blood sugar and fibrinogen showed a measurable lowering in the low plane animals. Age changes have been noted that are independent of nutritive state but are similar to those reported for domestic ruminants.

scholarly journals Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila

2021 ◽  
Vol 8 (1) ◽  
pp. 40-47
Author(s):  
Eric Armando ◽  
Ayu Lestiyani ◽  
R. Adharyan Islamy
Keyword(s):  
Body Weight ◽  
Plasma Protein ◽  
Lysozyme Activity ◽  
Total Plasma ◽  
Immune Parameters ◽  
Randomized Design ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
And Control ◽  
Range Test

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.

scholarly journals Changes in IgG and total plasma protein glycomes in acute systemic inflammation

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Mislav Novokmet ◽  
Edita Lukić ◽  
Frano Vučković ◽  
Željko –Durić ◽  
Toma Keser ◽  
...  
Keyword(s):  
Systemic Inflammation ◽  
Plasma Protein ◽  
Total Plasma ◽  
Total Plasma Protein
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