scholarly journals Intake, digestion and small intestinal protein availability in sheep in relation to ammoniation of wheat straw with or without protein supplementation

1995 ◽  
Vol 74 (3) ◽  
pp. 347-368 ◽  
Author(s):  
S. J. Oosting ◽  
J. Van Bruchem ◽  
X. B. Chen

The effects of ammoniation of wheat straw with or without supplementation of protein sources of either high (casein) or relatively low (potato protein) rumen degradability on intake and digestion were studied with four sheep in a 4 × 4 Latin square design. Rations offered were: (1) untreated wheat straw (UWS), (2) ammoniated wheat straw (AWS), (3) AWS supplemented with 3·2 g casein/kg live weight (W)0·75 per d (AWSC) and (4) AWS supplemented with 3·9 g potato protein/kg W0·75per d (AWSP). Straw was offered ad lib. and all rations were supplemented with sugarbeet pulp and a mineral mixture. NH3 treatment increased intake and digestion. Supplementation of AWS with potato protein increased total digestible organic matter intake (DOMI) compared with AWS whereas supplementation with casein did not affect total DOMI. Protein supplementation of AWS significantly reduced rumen digestion of cellulose, and when the supplementation was with casein it reduced rumen digestion of neutral-detergent fibre and hemicellulose also. This lower rumen digestion was compensated by a higher proportion of digestion occurring in the hindgut for hemicellulose (P<0·05 for AWSC, P>0·05 for AWSP), but not for cellulose. Across all rations, rumen fluid volume increased with increasing cell-wall intake. The efficiencies of microbial protein synthesis were (average of three different methods of estimation) 23·3, 26·2, 34·8 and 31·7gN/kg apparently-rumen-degraded organic matter for UWS, AWS, AWSC and AWSP respectively. The difference between UWS and AWS was not significant, but values for AWSC and AWSP were significantly higher than that for AWS. The rumen digestion of feed aimno acid-N (AA-N) was significantly higher for AWSC than for the other rations. The apparent small-intestinal digestion of AA-N and N was significantly higher for AWSP than for the other rations. The true small-intestinal digestion values were 0·86, 0·84 and 0·68 for AA-N, N and non-protein-N respectively. Heal endogenous losses of AA-N were approximately 6 mg/g duodenal non-protein dry-matter flow. Linear relationships were observed between DOMI and N balance and truly absorbed AA-N, indicating that DOMI could have been limited by small-intestinal amino acid availability. Regression of N balance v. truly absorbed AA-N resulted in an estimate of net efficiency of utilization of truly absorbed AA-N of 0·54.

1982 ◽  
Vol 30 (3) ◽  
pp. 391 ◽  
Author(s):  
DW Dellow ◽  
ID Hume

Intake and digestion of chopped lucerne hay was studied in three macropodine species, eastern grey kangaroo Macropus giganteus. red-necked pademelon Thylogale thetis and tammar wallaby M. eugenii, and in sheep. The utilization of fresh Phalaris grass by the two wallaby species T. thetis and M. eugenii was also examined and compared. On the chopped lucerne hay diet, intake of organic matter (OM) was similar in sheep and M. giganteus, but sheep digested more (P<0.05) OM and thus consumed more (P<0.05) digestible energy (DE) than all three macropodine species. Among the macropodines. M. eugenii consumed the least (P<0.01) OM and DE but digested more (P<0.05) OM. However, acid detergent fibre (ADF) digestibility was similar in the three macropodine species and lower (P<0.05) than in sheep. On the fresh grass diet M. eugenii consumed less (P<0.05) OM and DE than T. thetis. ADF digestibility was similar in both species. Although ,M. eugenii consumed less nitrogen (N) than the other three species on the lucerne diet (P<0.01) and less N than T. thetis on the Phalaris diet (P<0.05), N balance was similar and positive in all species. Data from this and other published studies indicate that the maintenance requirements for energy and nitrogen of most macropodines are lower than for sheep, and appear to be lower in M, eugenii than in any other macropodine studied so far. It is also apparent that there are probably few real differences among the Macropodinae in efficiency of fibre digestion, at least on diets of adequate nitrogen and DE content.


1994 ◽  
Vol 72 (1) ◽  
pp. 147-165 ◽  
Author(s):  
S. J. Oosting ◽  
P. J. M. Vlemmix ◽  
J. Van Bruchem

Untreated wheat straw (UWS) or ammoniated wheat straw without (AWS) or with (AWSP) a supplement of potato protein of a low rumen degradability was fed to three steers according to a 3 × 3 Latin square design. All rations were supplemented with sugar-beet pulp and minerals. Voluntary organic matter intake (OMI, g/kg0.75 per d) was 67.8, 76.0 and 80.1 for whole rations (51.1, 59.7 and 59.2 for straw) for UWS, AWS and AWSP respectively, which was significantly higher for AWS and AWSP than for UWS. Organic matter digestibility (OMD, g/kg) was 561, 596 and 625 for the respective rations UWS, AWS and AWSP, also significantly higher for AWS and AWSP than for UWS. The increased voluntary intake and digestion of ammoniated wheat-straw-based rations were associated with a significantly higher potentially degradable fraction (D) of neutral detergent fibre (NDF) in offered straw (556 and 661 g/kg for untreated and ammoniated wheat straw respectively) and in the rumen pool (469, 555 and 554 g/kg for UWS, AWS and AWSP respectively). Isolated small rumen particles (retained on sieves with a pore size < 1.25 and > 0.041 mm) had a significantly lower D of NDF (average 588 g/kg) than isolated large rumen particles (average 663 g/kg). Fractional rates of degradation of NDF did not differ significantly either between untreated and ammonia-treated wheat straw offered (2.9 and 2.6%/h respectively) or between rumen pools (1.8, 1.7 and 2.1 %/h for UWS, AWS and AWSP respectively). Rations based on ammoniated wheat straw had a significantly higher rumen NH3-N concentration than UWS. Although the rumen pool size of total contents differed significantly between treatments, those of dry and organic matter and of cell wall constituents were not significantly different. The proportion of rumen dry matter passing through a sieve with a pore size of 1.25 mm averaged 0.684 over rations (not significantly different between rations). Daily rumination (96 min) and eating (52 min) times/kg NDF ingested did not differ between rations. The rate of comminution of large particles estimated from the disappearance of indigestible NDF in large rumen particles from the rumen of animals without access to feed was 4.1, 6.3 and 7.1 %/h for UWS, AWS and AWSP respectively. These values were not significantly different. The fractional rate of passage estimated from the faecal excretion of Cr-NDF was 5.4, 6.1 and 6.3%/h for UWS, AWS and AWSP respectively (significantly higher for AWS and AWSP than for UWS) but the turnover rate of indigestible NDF did not differ between treatments.


1972 ◽  
Vol 78 (1) ◽  
pp. 7-12 ◽  
Author(s):  
M. Freer ◽  
D. B. Jones ◽  
K. R. Christian

SUMMARYIn each of two experiments the comparative slaughter technique was used to compare the retention of energy by young Border Leicester × Merino wethers when the same total amount of food was offered according to two different feeding regimens. These were designed to simulate, in pens with a diet of pelleted lucerne, feeding patterns typical of continuous and intensive rotational grazing.In the first experiment ten 9-month-old sheep that were offered the same amount of food daily (mean intake 367 g digestible organic matter) during each of 14 weeks made mean daily gains of 39 g live weight, 6·3 g body fat and 0·59 MJ (140 kcal) energy compared with a gain of 30 g live weight, a loss of 1·4 g body fat and a gain of 0·28 MJ (67 kcal) energy by a similar group that ate the same total quantity of food but in amounts that ranged, during each week, from 1·8 to 0·1 times the daily intake of the other group. This cyclic feeding pattern caused a small decrease in the digestibility of dietary organic matter.In the second experiment two similar feeding patterns were compared with ten pairs of 3-month-old sheep eating twice as much food each week as in the first experiment. One member of each pair was, in each of 13 weeks, offered food ad lib. for 3 days and then amounts falling to 20% of voluntary intake on the seventh day; the other one ate the same amount of food each week but in seven almost equal feeds. The latter sheep gained, on average, 132 g live weight daily and retained 58 g body fat and 2·96 MJ (707 kcal) energy compared with 124 g live weight, 49 g body fat and 2·61 MJ (624 kcal) energy by the sheep on the cyclic feeding pattern. In neither experiment was wool growth affected by feeding pattern.The greater effect of the cyclic feeding pattern in reducing the efficiency of energy retention in the first experiment is attributed to the higher number of days each week during which these animals were estimated to be in negative energy balance. These results suggest that adverse nutritional effects of a fluctuating feeding pattern in rotational grazing are likely to be most important where sheep are being rationed near their maintenance level and only small where food is ample.


1969 ◽  
Vol 72 (2) ◽  
pp. 223-228 ◽  
Author(s):  
J. F. D. Greenhalgh ◽  
G. W. Reid

SUMMARYOn three occasions in May—July one half of a ryegrass pasture was grazed intensively by cattle and the other was mown. In August and September these areas, designated fouled (F) and clean (C) respectively, were each strip-grazed by two groups of five cows. One group on each area had a herbage allowance of 11·4 kg dry matter per cow per day (C11 and F11) and the other 20·4 kg (C20 and F20).Before the August-September grazing the fouled area had 2·8% of its surface covered by faeces. After this grazing 23 % of the F n area and 34 % of the F20 area were classified as having been rejected by the cows. However, the upper parts of the sward on these rejected areas were in fact grazed. There were only small differences in N and soluble-carbohydrate contents between the herbages offered and rejected.Organic-matter digestibility (%) and intake (kg/cow per day) for the four groups were: C11, 73·0 and 10·5; C20, 74·9 and 11·7; F11 , 74·4 and 9·6; F20, 77·0 and 10·6. The C11 cows consumed all the herbage allowed to them, but the F11 rejected 13%. Digestible organic matter intake was affected more by grazing intensity than by fouling; this was the case also for milk yield, milk composition and the live-weight change of the cows.


1980 ◽  
Vol 44 (3) ◽  
pp. 343-354 ◽  
Author(s):  
R. G. Redman ◽  
R. C. Kellaway ◽  
Jane Leibholz

1. Expt 1. Five 150 kg steers with ruminal, abomasal and ileal cannulas were given 3000 g oaten chaff daily plus prelleted supplement with no added nitrogen (diet A) or 50 g N/d as urea (diet B), casein (diet C), casein and formaldehyde-treated casein (HCHO-casein) (50:50 w/w; diet D) and HCHO-casein (diet E), in a 5 × 5 Latin square design. The basal diet and supplement were fed in eight equal increments at intervals of 3 h. Proportions of dry matter and organic matter digested in the stomach and whole tract were greater for diets B, C, D and E than for diet A. Total volatile fatty acid levels in the rumen and the proportion of acetic acid were lower, and the proportion of propionic acid higher on diet A than on the other diets. Rumen ammonia levels were lower on diets A, D and E than on diets B and C. N flows at the abomasum, ileum and rectum were lower on diet A than on the other diets; abomasal flows and apparent intestinal absorptions of amino acids were higher on diets D and E than on diets A, B and C. Efficiencies of bacterial protein synthesis were 15, 15, 14, 13 and 12 g bacterial N/kg OM truly digested in the stomach on diets A, B, C, D and E respectively.2. Expt. 2. Forty 300 kg steers were fed oaten chaffad lib.plus twice the amount of the same pelleted supplements as in Expt 1. Intake of oaten chaff was 23% higher with N supplements (diets B, C, D and E) than without (diet A). Live-weight gains were 356, 798, 843, 842 and 805 g/d on diets A, B, C, D and E respectively.3. It was concluded that efficiency of bacterial protein synthesis was not limited by the supply of peptides and amino acids in the rumen and that increases in amino acid availability in the intestines from feeding HCHO-casein did not increase food intake or live-weight gain.


2008 ◽  
Vol 17 (2) ◽  
pp. 109 ◽  
Author(s):  
A. HUUSKONEN ◽  
H. KHALILI ◽  
E. JOKI-TOKOLA

The objective of the present experiment was to study the need for the protein supplementation in the diet of growing dairy bulls (initial live weight 272 ± 28.5 kg and final live weight 666 ± 31.2 kg, on average) fed total mixed ration based on moderate digestible grass silage and barley. The experiment comprised 24 Finnish Ayrshire bulls and 8 Holstein-Friesian bulls and included four treatments. The control diet (C) consisted of moderate digestible (653 g digestible organic matter in dry matter (DM) grass silage (450 g kg-1 DM), barley grain (275) and barley fibre (275) without protein supplementation. Three isonitrogenous experimental diets included also extra protein, i.e. (1) rapeseed meal (RSM) (supplementation 530 g DM per animal day-1), (2) wet distillers’ solubles (WDS) (600 g) and (3) a mixture of barley protein (90% of fresh weight) and wet distillers’ solubles (10) (BPWDS) (480 g). In all isonitrogenous diets the crude protein content of concentrate increased from 137 to 150 g kg-1 DM (9%) compared with the C diet. All bulls were fed total mixed ration ad libitum. The energy content of all diets was 11.6 MJ kg-1 DM. The live weight gain of the bulls tended to be higher with the BPWDS diet than with the C diet (C 1214 vs. BPWDS 1301 g d-1; p = 0.10), but the treatments had no significant effect on carcass gain, feed conversion or slaughter parameters. Only the BPWDS diet differed significantly from the C diet in DM (C 9.69 vs. BPWDS 10.38 kg DM d-1; p < 0.01) and energy intake (C 112.4 vs. BPWDS 120.3 MJ d-1; p < 0.05). The apparent organic matter digestibility (OMD) was 5% higher in the BPWDS diet than in the C diet (p < 0.001), but the RSM and WDS diets did not differ from the C diet in OMD. The results indicate that the supply of protein in dairy bulls is most probably adequate with moderate digestible, well-preserved grass silage and barley-based concentrates when intake of digestible organic matter is high enough to support microbial protein synthesis in the rumen.;


1983 ◽  
Vol 100 (3) ◽  
pp. 657-666 ◽  
Author(s):  
D. W. Hennessy ◽  
P. J. Williamson ◽  
J. V. Nolan ◽  
T. J. Kempton ◽  
R. A. Leng

SUMMARYTwenty-seven Hereford weaner steers (mean age 11 months, and live weight 142±17 kg) were used to study the effect of supplementation of an energy-rich grain (sorghum), and/or a protein meal on the live-weight change of cattle on a basal diet of low-quality grass hay over 48 days (Expt 1). The basal hay had an estimated energy value of 6·2 MJ metabolizable energy (ME)/kg dry matter (D.M.) and a nitrogen content of 6·2 g/kg D.M. Corresponding values for the sorghum supplement were 12·4 and 14·4, and for the protein meal 9·6 and 65. The daily rates of supplementation in Expt 1 were (g/head) 0 (nil), 600 (P1) or 1200 (P2) of protein meal pellets or 0 (nil), 560 (S1) or 1120 (S2) of sorghum and all combinations of P1or P2with S1or S2. The carry-over effect of such supplementation on subsequent growth was recorded in Expt 2 when the steers grazed a kikuyu grass pasture for 100 days.In Expt 1, organic-matter intake of hay (HOMI) was increased (P< 0·01) by protein but decreased (P< 0·01) by the sorghum supplements. By 48 days, P1-supplemented steers were eating 29·5% more hay than non-supplemented steers, and P2steers were eating 43·5% more hay than the non-supplemented steers. S1supplemented steers were eating 29·5% less, and S2steers 23% less hay than non-supplemented steers. Steers lost weight on the basal hay diet, and at the lower level of sorghum (S1) supplementation, but were able to maintain weight at the higher level (S2) of supplementation. In contrast, steers supplemented with protein gained weight (P< 0·01): 430 g/day on P1and 700 g/day on P2treatments. None of the steers offered combinations of P and S had live-weight changes as high as those offered only protein supplements. There was no evidence of compensatory gain by the smaller steers in Expt 2 when grazing and unsupplemented.Glucose kinetics in plasma were studied in each steer on day 35 of Expt 1 by means of a single intravenous injection of [2-3H]glucose. The quantity of glucose in the sampled pool and the total rate of glucose entry into the pool (GER) were higher (P< 0·01) in steers that were protein supplemented, with GER being related to both ME intake and to N intake per unit ME intake.Urea pool size and entry rate were also estimated on day 35 of Expt 1 using a single intravenous injection of [14C]urea. The quantity of urea in the sampled pool, and entry rate into the pool were increased significantly (P< 0·01) by protein, but reduced (P< 0·05) by sorghum, supplementation. Rumen ammonia concentrations were low in steers given the hay alone, and when supplemented with sorghum (10–15 mg N/l), but were significantly higher (70 mg N/l;P< 0·01) during protein supplementation.It was concluded that growth of cattle on the basal ration was restricted by low voluntary intakes of hay. Intake was increased substantially by protein supplementation. The consequent increase in live weight was due to a greater intake of organic matter which was accompanied by a greater synthesis of glucose and availability of amino acids in the animals.


2021 ◽  
pp. 1-11
Author(s):  
K. Itani ◽  
J. Ø. Hansen ◽  
B. Kierończyk ◽  
A. Benzertiha ◽  
P. P. Kurk ◽  
...  

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1132
Author(s):  
Sarah Schwarzkopf ◽  
Asako Kinoshita ◽  
Jeannette Kluess ◽  
Susanne Kersten ◽  
Ulrich Meyer ◽  
...  

Development of calves depends on prenatal and postnatal conditions. Primiparous cows were still maturing during pregnancy, which can lead to negative intrauterine conditions and affect the calf’s metabolism. It is hypothesized that weaning calves at higher maturity has positive effects due to reduced metabolic stress. We aimed to evaluate effects of mothers’ parity and calves’ weaning age on growth performance and blood metabolites. Fifty-nine female Holstein calves (38.8 ± 5.3 kg birth weight, about 8 days old) were used in a 2 × 2 factorial experiment with factors weaning age (7 vs. 17 weeks) and parity of mother (primiparous vs. multiparous cows). Calves were randomly assigned one of these four groups. Live weight, live weight gain and morphometry increased over time and were greater in calves weaned later. Metabolic indicators except total protein were interactively affected by time and weaning age. Leptin remained low in early-weaned calves born to primiparous cows, while it increased in the other groups. The results suggest that weaning more mature calves has a positive effect on body growth, and calves born to primiparous cows particularly benefit from this weaning regimen. It also enables a smooth transition from liquid to solid feed, which might reduce the associated stress of weaning.


1985 ◽  
Vol 40 (1) ◽  
pp. 101-109 ◽  
Author(s):  
R. J. Orr ◽  
T. T. Treacher ◽  
V. C. Mason

ABSTRACTFinnish Landrace × Dorset Horn ewes were offered 300, 600 or 900 g fresh weight per day of concentrates and forage ad libitum from day 105 of pregnancy until lambing. Spring barley straw (S) or hay (H) was offered either untreated (U) or following treatment with anhydrous ammonia in an oven (T). Organic matter digestibilities (in vitro) were 0·42, 0·58, 0·42 and 0·60 and nitrogen contents were 7·2, 18·6, 12·0 and 25·0 g/kg dry matter for US, TS, UH and TH respectively. Forage intake did not differ between ewes carrying two or more foetuses but the small number of ewes carrying one foetus ate more straw (6·8 v. 4·5 g organic matter (OM) per kg live weight) than ewes carrying two or more foetuses. Ammonia treatment increased intake; the increase was larger on straw (4·6 v. 100 g OM per kg live weight) than on hay (9·0 v. 10·7 g OM per kg live weight). Replacement rates of forage by concentrates were -0·21, +0·06, -0·48 and +0·08 kg forage per kg concentrates for treatments US, TS, UH and TH respectively; only the value for treatment UH differed significantly from zero. On most treatments forage intake decreased as pregnancy progressed and the declines were greater when treated forages were offered. Concentrate level had a large effect on most aspects of ewe performance. Ewes offered treated forage gained slightly more weight in pregnancy (138 v. 104 g/day), had a slightly smaller decrease in body condition score (-0·54 v. -0·68) between day 105 and lambing but did not have greater lamb birth weights than ewes on untreated forage.


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