scholarly journals Effect of protein nutrition on the mRNA content of insulin-like growth factor-binding protein-1 in liver and kidney of rats

1993 ◽  
Vol 69 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Asako Takenaka ◽  
Mitsuko Hirosawa ◽  
Masamichi Mori ◽  
Sanae Yamada ◽  
Yutaka Miura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Casein Diet ◽  
Liver Parenchymal ◽  
Factor Binding ◽  
Mrna Content ◽  
Liver And Kidney ◽  
Protein Free Diet ◽  
Parenchymal Cells

Effect of quantity and nutritional quality of dietary proteins on the content of mRNA of insulin-like growth factor-binding protein-1 (IGFBP-1) was studied in rat liver and kidney. IGFBP-1 mRNA content per unit RNA increased in liver and kidney of rats fed on a protein-free diet and in those of fasted rats compared with that in the rats fed on a casein diet. When rats were given a gluten diet for 7 d, IGFBP-1 mRNA content in liver did not change significantly but that in kidney increased considerably compared with that in those organs of the rats fed on the casein diet. Because IGFBP-1 mRNA has been demonstrated both in liver parenchymal and non-parenchymal cells (Takenaka et al. 1991), the effect of the protein-free diet on these two types of cells has been studied. An increase in IGFBP-1 mRNA content under protein deprivation was observed in both liver parenchymal and non-parenchymal cells, suggesting that these two types of cells are regulated in a similar mode as far as IGFBP-1 mRNA content is concerned. The physiological and nutritional significance of the previously stated results on protein anabolism are discussed when considered together with our previous observations on the plasma concentrations of IGF-1 (Takahashi et al. 1990) and IGFBP (Umezawa et al. 1991) and insulin-like growth factor-1 mRNA content in liver (Miura et al. 1991).

scholarly journals Effect of dietary proteins on insulin-like growth factor-1 (IGF-1) messenger ribonucleic acid content in rat liver

1992 ◽  
Vol 67 (2) ◽  
pp. 257-265 ◽  
Author(s):  
Yutaka Miura ◽  
Hisanori Kato ◽  
Tadashi Noguchi
Keyword(s):  
Growth Factor ◽  
Rat Liver ◽  
Soya Bean ◽  
Dietary Proteins ◽  
Insulin Like Growth Factor ◽  
Casein Diet ◽  
Mrna Species ◽  
Mrna Content ◽  
Protein Free Diet

Effects of quantity and quality of dietary proteins on plasma immunoreactive insulin-like growth factor-1 (IGF-1) concentration, and content of IGF-1 mRNA in rat liver were investigated in rats. Plasma immunoreactive IGF-1 concentration in rats given a casein diet was higher than that in rats given a soya-bean-protein or protein-free diet. The IGF-1 mRNA content in liver was estimated by the Northern blot hybridization technique employing 32P-labelled rat IGF-1 complementary DNA (cDNA). At least four molecular species of IGF-1 mRNA of different molecular weight were found in rat liver. The sizes were 0·8–1·2, 2·0, 3·6–4·0 and 7·4 kb. Most of the mRNA species decreased in the livers of rats given a gluten diet (120 g gluten/kg diet) compared with rats given the casein diet. In particular, mRNA of 7·4 kb decreased markedly. When rats were fed on the protein-free diet, mRNA of all species decreased significantly. The estimated IGF-1 mRNA in the livers of rats fed on the gluten or protein-free diet was almost 0·4 of that of the rats given the casein diet. Feeding the soya-bean-protein diet did not result in a marked effect on the hepatic content of mRNA species of IGF-1. The results showed that liver IGF-1 mRNA content is sensitively regulated by quantity and nutritional quality of dietary proteins.

Regulation of insulin-like growth factor-binding protein-1 in rat serum

Diabetes ◽  
1994 ◽  
Vol 43 (2) ◽  
pp. 232-239 ◽  
Author(s):  
M. S. Lewitt ◽  
H. Saunders ◽  
J. L. Phyual ◽  
R. C. Baxter
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Rat Serum ◽  
Factor Binding
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