scholarly journals The effects of acute and chronic administration of n-6 and n-3 polyunsaturated fatty acids on ethanol-induced gastric haemorrhage in rats

1992 ◽  
Vol 67 (3) ◽  
pp. 501-507 ◽  
Author(s):  
B. Hunter ◽  
G. S. A. McDonald ◽  
M. J. Gibney
Keyword(s):  
Fish Oil ◽  
Micellar Solution ◽  
Wistar Rats ◽  
Gastric Lesion ◽  
Control Diet ◽  
Chronic Administration ◽  
Standard Laboratory ◽  
Weanling Rats ◽  
Female Wistar Rats ◽  
Maize Oil

Female weanling rats in three equal groups (n12) were given orally by intubation 1 ml micellar solution of taurocholic aicd (10 mM) and either arachidonic acid (20:4n−6), linoleic acid (18:2n−6) or eicosapentaenoic acid (20:5n−3) at a concentration of 120 mM. After 1 h the rats were given intragastrically 2 ml absolute ethanol and were killed 1 h later. Rats given oral 20:4n−6 showed a significant reduction (P< 0.05) in the extent (%) of gastric mucosal haemorrhage compared with either the rats given 20:5n−3 or 18:2n−6 (8.3 (SD 7.3), 23.2 (SD 10.4) and 21.4 (SD 10.4)) respectively. In a second experiment, four equal groups (n12) of female Wistar rats were fed for 5 weeks on either a control diet of standard laboratory rat food, or the same diet enriched with either maize oil or fish oil or butterfat at a level of 100 g/kg. Following a 24 h fast the rats received an intragastric dose of 2 ml ethanol and were killed 1 h later. Examination of the extent (%) of gastric lesion showed a significant reduction (P< 0.05) with the feeding of either maize oil or fish oil compared with the controls (12.2 (SD 8.2), 15.3 (SD 13.2) and 29.3 (SD 14.0) respectively). The butterfat diet was not significantly different from the control diet (23.8 (SD 8.1)).

Isoflurane but not halothane minimum alveolar concentration-sparing response of dexmedetomidine is enhanced in rats chronically treated with selective α2-adrenoceptor agonist

2012 ◽  
Vol 46 (3) ◽  
pp. 215-219
Author(s):  
M Santos ◽  
J A Ibancovichi ◽  
I Millán ◽  
F J Tendillo
Keyword(s):  
Wistar Rats ◽  
Low Dose ◽  
Saline Solution ◽  
Minimum Alveolar Concentration ◽  
Chronic Administration ◽  
Naive Group ◽  
Adrenoceptor Agonist ◽  
Female Wistar Rats ◽  
Intraperitoneal Dose ◽  
Sparing Effect

Halothane minimum alveolar concentration (MAC)-sparing response is preserved in rats rendered tolerant to the action of dexmedetomidine. It has been shown that halothane and isoflurane act at different sites to produce immobility. The authors studied whether there was any difference between halothane and isoflurane MAC-sparing effects of dexmedetomidine in rats after chronic administration of a low dose of this drug. Twenty-four female Wistar rats were randomly allocated into four groups of six animals: two groups received 10 μg/kg intraperitoneal dexmedetomidine for five days (treated groups) and the other two groups received intraperitoneal saline solution for five days (naive groups) prior to halothane or isoflurane MAC determination (one treated and one naive group of halothane and one treated and one naive group of isoflurane). Halothane or isoflurane MAC determination was performed before (basal) and 30 min after an intraperitoneal dose of 30 μg/kg of dexmedetomidine (post-dex) from alveolar gas samples at the time of tail clamp. Administration of an acute dose of dexmedetomidine to animals that had chronically received dexmedetomidine resulted in a MAC-sparing effect that was similar to that seen in naive animals for halothane; however, the same treatment increased the MAC-sparing response of dexmedetomidine for isoflurane. Isoflurane but not halothane MAC-sparing response of acutely administered dexmedetomidine is enhanced in rats chronically treated with this drug.

scholarly journals The influence of different amounts ofn-3 polyunsaturated fatty acids on bleeding time and invivovascular reactivity

1994 ◽  
Vol 71 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Gwenda Mark ◽  
T. A. B. Sanders
Keyword(s):  
Fish Oil ◽  
Wistar Rats ◽  
Vascular Reactivity ◽  
Bleeding Time ◽  
Control Diet ◽  
Vessel Diameter ◽  
Similar Increase ◽  
Mesenteric Vessel ◽  
Lipid Fatty Acid ◽  
Erythrocyte Lipid

Mesenteric bleeding time, mesenteric vascular reactivity, platelet and erythrocyte lipid fatty acid composition were measured at 2–3 weeks, 5–6 weeks and 11–22 weeks in normotensive Wistar rats, fed on high (6·5% energy) or moderate (1·6% energy) intakes of eicosapentaenoic acid (20: 5n−3; EPA) as fish oil, compared with controls fed on a diet devoid of EPA. All diets contained the same level of linoleic acid (4% energy): the moderate- and high-EPA diets also contained 1·1 and 4·4% of the energy as docosahexaenoic acid (22:6n−3) respectively. Moderate, but not high, intakes of EPA increased mesenteric bleeding time. Similar reductions in erythrocyte and platelet arachidonic acid (20: 4n−6) occurred in animals fed on either high or low amounts of EPA, but the proportion of EPA increased dose-dependently. At high intakes of EPA the proportion of oleic acid in platelets and erythrocytes was decreased. Blood pressure platelet counts, mesenteric vessel diameter and mesenteric vascular reactivity to vasopressin were unaffected by treatment. High intakes of fish oil led to a slight fall in packed cell volume. In a second experiment bleeding time and mesenteric vascular reactivity to noradrenaline were increased 2–4 weeks after receiving a moderate intake of EPA and these effects persisted 5–21 d after switching to a control diet. A similar increase in vascular reactivity to noradrenaline was observed in animals given indomethacin (6 mg/kg) but not in those given aspirin (20 mg/kg).

Differential Effects of Fatty Acids and Exercise on Body Weight Regulation and Metabolism in Female Wistar Rats

2003 ◽  
Vol 228 (7) ◽  
pp. 843-849 ◽  
Author(s):  
K-L. Catherine Jen ◽  
Anne Buison ◽  
Michael Pellizzon ◽  
Frank Ordiz ◽  
Lardo Santa Ana ◽  
...  
Keyword(s):  
Body Weight ◽  
Insulin Sensitivity ◽  
Fish Oil ◽  
Soybean Oil ◽  
Palm Oil ◽  
Wistar Rats ◽  
Weight Regulation ◽  
Body Weight Regulation ◽  
High Fat ◽  
Female Wistar Rats

High-fat diets made with different fats may have distinct effects on body weight regulation and metabolism. In the present study, the metabolic effects of high-fat (HF) diets made with fish oil, palm oil, and soybean oil were compared with a low-fat diet in female Wistar rats that were either exercised (EX, swimming) or that remained sedentary as controls. Each adult rat was exposed to the same diet that their dams consumed during pregnancy and lactation. When they were 9 weeks old, rats began an EX regimen that lasted for 6 weeks. Twenty-four hours after the last EX bout, rats were sacrificed in a fasted state. It was observed that HF feeding of soybean oil induced more body weight and fat gain, as well as insulin resistance, as indicated by insulin/glucose ratios, than other oils. Female rats fed a HF diet made with fish oil had body weight and insulin sensitivity not different from that observed in low fat fed control rats. For rats fed HF diets made with soybean oil or palm oil, EX also exerted beneficial effects by reducing body fat %, blood insulin, triglyceride and leptin levels, as well as improving insulin sensitivity.

scholarly journals The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study

2020 ◽  
Vol 77 (6) ◽  
pp. 641-646
Author(s):  
Nevena Vidovic ◽  
Slavica Rankovic ◽  
Zorana Orescanin-Dusic ◽  
Jasmina Debeljak-Martacic ◽  
Tamara Popovic ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Liver Tissue ◽  
Wistar Rats ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Diet Group ◽  
Control Group ◽  
Omega 3 ◽  
Dietary Antioxidants ◽  
Female Wistar Rats

Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ? 0.73 U/mg) and CAT (147.25 ? 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ? 0.11 U/mg; CAT: 99.37 ? 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ? 0.52 U/mg; CAT: 104.18 ? 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ? 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ? 0.08 nmoL/mg; the fish-based diet group: 1.13 ? 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one?s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants.

scholarly journals A Hyperlipidic Diet Combined with Short-Term Ovariectomy Increases Adiposity and Hyperleptinemia and Decreases Cytokine Content in Mesenteric Adipose Tissue

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Nelson Inacio Pinto Neto ◽  
Maria Elizabeth Sousa Rodrigues ◽  
Ana Claudia Losinskas Hachul ◽  
Mayara Franzoi Moreno ◽  
Valter Tadeu Boldarine ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Control Diet ◽  
The Body ◽  
Short Term ◽  
Serum Leptin ◽  
Sham Surgery ◽  
Female Wistar Rats ◽  
Hyperlipidic Diet

Four-week-old female Wistar rats were divided into two groups and fed a control diet (C) or a hyperlipidic diet (H) for 4 weeks. Rats from each group underwent ovariectomy (OVX) or sham surgery (SHAM). They received C or H for the next four weeks. The body weight gain (BW), food efficiency (FE), and carcass lipid content were higher in the OVX H than in the SHAM H. The OVX H exhibited a higher serum leptin level than other groups. IL-6, TNF-α, and IL-10 content of mesenteric (MES) adipose tissue was lower in the OVX H than in the OVX C. IL-6, TNF-α, and IL-10 content of retroperitoneal (RET) adipose tissue was lower in the SHAM H than in the SHAM C. The SHAM H showed decreased TG relative to the SHAM C. Similar results were obtained in relation to IL-6Rα, TNFR1, TLR-4, and MyD88 contents in the MES and RET white adipose tissue among the groups. A hyperlipidic diet for 8 weeks combined with short-term ovariectomy decreases the cytokine content of MES adipose tissues but increases BW, enhancing FE and elevating serum leptin levels. These suggest that the absence of estrogens promotes metabolic changes that may contribute to installation of a proinflammatory process induced by a hyperlipidic diet.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

Oral in vivo Bactofection in Dextran Sulfate Sodium Treated Female Wistar Rats

2010 ◽  
Vol 58 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Roland Pálffy ◽  
Michal Behuliak ◽  
Roman Gardlík ◽  
Peter Jáni ◽  
L'udevít Kádaši ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Female Wistar Rats
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