scholarly journals An evaluation of the phytate, zinc, copper, iron and manganese contents of, and Zn availability from, soya-based textured-vegetable-protein meat-substitutes or meat-extenders

1979 ◽  
Vol 41 (3) ◽  
pp. 579-589 ◽  
Author(s):  
N. T. Davies ◽  
Hilary Reid
Keyword(s):  
Trace Element ◽  
Vegetable Protein ◽  
Molar Ratio ◽  
Lower Growth ◽  
Protein Nitrogen ◽  
Zn Content ◽  
Zn Status ◽  
Textured Vegetable Protein ◽  
Iron Manganese ◽  
Phytate Content

1. A study has been made of the zinc, copper, iron, manganese, protein (nitrogen x 625) and phytic acid contents of nineteen soya-bean-based textured-vegetable-protein (TVP) meat-extenders and meat-substitutes and of three ‘ready-prepared’ canned meals containing TVP.2. Phytate analysis was performed using a newly-developed method based on Holt's (1955)procedure. This method enabled the phytate content of milligram quantities of TVP to be estimated, with an SD for six replicates of 3%.3. The Fe, Cu and Mn contents (mg/kg) of the meat-extenders or meat-substitutes varied, wzith values of 59.4–144, 14.1–19.7 and 19.5–29.1 respectively. The protein content of these products was approximately 500 g/kg.4. The phytate content of the meat-extenders and meat-substitutes ranged from 11.0to 20.2 g/kg and the Zn content from 35.0 to 49.4 mg Zn/kg. The calculated molar ratio, phytate:Zn varied from 25 to 42.5. The trace element, phytate and protein contents of the ‘ready-prepared’ canned meals were 30–50 %: lower than the meat-extenders and meat-substitutes.6. Cooking the ‘ready-prepared’ meals as specified by the manufacturers was without effect on the trace element or phytate content.7. When TVP was fed to rats as the only protein source, they had significantly lower growth rates and plasma Zn concentrations than rats given an egg-albumen-based diet of similar Zn content (14.5mg Zn/kg). Supplementation of the TVP diet with Zn (100 mg Zn/kg) significantly increased growth rate and plasma Zn concentration whereas Zn supplementation of the albumen diet was without effect.8. The possible implications of consumption of TVP products in relation to Zn status of the human population is discussed.

Studies on the phytate: zinc molar contents in diets as a determinant of Zn availability to young rats

1979 ◽  
Vol 41 (3) ◽  
pp. 591-603 ◽  
Author(s):  
N. T. Davies ◽  
S. E. Olpin
Keyword(s):  
Weight Gain ◽  
Fixed Amount ◽  
Sodium Phytate ◽  
Egg Albumin ◽  
Zn Content ◽  
Egg Albumen ◽  
Zn Status ◽  
Textured Vegetable Protein ◽  
Zn Availability

1. Studies were carried out in vitro to examine the effects of phytate on the solubility of the trace elements zinc, copper and manganese. Appropriate volumes of a solution of sodium phytate were added to a mineral solution to achieve phytate: Zn values of from 0: 1 to 45:1. In a second series the same values for phytate: Zn were achieved by varying the amount of added Zn at a fixed phytate concentration.2. In both experiments > 85% of the Zn was rendered insoluble at pH 6.5 even at the lowest value for phytate:Zn (5:1). The effect of phytate on Zn solubility was greater than effects on Cu or Mn.3. In a dietary study, rats were offered a semi-synthetic egg-albumin-based diet with added phytate. Two series of diets were prepared, the first had a constant Zn content (18.5 mg Zn/kg) and the amount of sodium phytate varied so as to achieve values for phytate: Zn of from 0:1 to 40:1 (series 1). In the second series, the same values for phytate:Zn were achieved by adding a fixed amount of phytate (7.4 g phytic acid/kg) while the amount of Zn was varied (series 2).4. Dietary phytate caused significant reductions in growth rates, plasma Zn concentrations and hair Zn concentrations and greying of the coat at values for phytate:Zn of 15:1, 10:1, 10:1 and 15:1, respectively.5. While phytate was apparently slightly more effective in reducing Zn status when phytate:Zn values were achieved at the lower absolute levels of phytate and Zn (series I diets), the differences at equivalent phytate:Zn values were small. It was concluded that phytate:Zn values can be used as an indicator of Zn availability from phytate-rich diets.Rats offered three diets containing soya-bean-based textured-vegetable-protein (TVP) exhibited low rates of weight gain compared with rats offered an egg-albumen-based diet of similar Zn content (14.5 mg Zn/kg). Additional Zn supplied in drinking-water (25 mg Zn/l) was without effect on rats consuming the egg-albumin diet but significantly improved the weight gain of rats on the TVP diets.7. It was concluded that phytate naturally present in TVP behaves similarly to phytate added to an otherwise phytate-free diet and that the reduced availability of Zn in TVP diets can be accounted for entirely by their phytate contents.

scholarly journals Improved functional properties of meat analogs by laccase catalyzed protein and pectin crosslinks

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kiyota Sakai ◽  
Yukihide Sato ◽  
Masamichi Okada ◽  
Shotaro Yamaguchi
Keyword(s):  
Sugar Beet ◽  
Free Amino ◽  
Vegetable Protein ◽  
Amino Nitrogen ◽  
Gastrointestinal Digestion ◽  
Binding Ability ◽  
Textured Vegetable Protein ◽  
Protein Crosslinks ◽  
Produce Plant ◽  
Shape And Size

AbstractThe gap between the current supply and future demand of meat has increased the need to produce plant-based meat analogs. Methylcellulose (MC) is used in most commercial products. Consumers and manufacturers require the development of other novel binding systems, as MC is not chemical-free. We aimed to develop a novel chemical-free binding system for meat analogs. First, we found that laccase (LC) synergistically crosslinks proteins and sugar beet pectin (SBP). To investigate the ability of these SBP-protein crosslinks, textured vegetable protein (TVP) was used. The presence of LC and SBP improved the moldability and binding ability of patties, regardless of the type, shape, and size of TVPs. The hardness of LC-treated patties with SBP reached 32.2 N, which was 1.7- and 7.9-fold higher than that of patties with MC and transglutaminase-treated patties. Additionally, the cooking loss and water/oil-holding capacity of LC-treated patties with SBP improved by up to 8.9–9.4% and 5.8–11.3%, compared with patties with MC. Moreover, after gastrointestinal digestion, free amino nitrogen released from LC-treated patties with SBP was 2.3-fold higher than that released from patties with MC. This is the first study to report protein-SBP crosslinks by LC as chemical-free novel binding systems for meat analogs.

scholarly journals Studies on the effects of dietary zinc dose on 65Zn absorption in vivo and on the effects of Zn status on 65Zn absorption and body loss in young rats

1987 ◽  
Vol 57 (1) ◽  
pp. 35-44 ◽  
Author(s):  
D. E. Coppen ◽  
N. T. Davies
Keyword(s):  
Gel Filtration ◽  
Male Rats ◽  
Whole Body ◽  
Gel Chromatography ◽  
Zn Content ◽  
Zn Status ◽  
Higher Doses ◽  
Obvious Relation ◽  
Second Peak

1. Weanling male rats were maintained on diets containing 5, 10, 20, 40, 80 or 160 mg zinc/kg for 14 d. On day 15 they received 65Zn either by intraperitoneal injection or in a test meal containing 20 mg Zn/kg. After dosing, the rats were again maintained on the diets they had received previously.2. Whole-body 65Zn retention was measured immediately after dosing and daily for a further 9 d. From regression analysis of the semi-logarithmic plots of 65Zn retention from 0 to 192 h after 65Zn administration, the true extent of 65Zn absorption and the biological half-life (t1/2) of body 65Zn stores were calculated.3. At the end of the experiment, the rats were killed and the entire small intestines of some rats from each group were rapidly flushed out to remove food and faecal residues, frozen in liquid nitrogen and stored under an atmosphere of N2 at –20° before separation of cytosolic Zn-binding fractions by gel filtration on Sephadex G–75.4. The results suggest that rats which received diets that were either deficient(5 mg Zn/kg), marginal (10 mg Zn/kg) or adequate (20–80 mg Zn/kg) in Zn achieved homeostatic regulation of body Zn by changes in both the extent of Zn absorption and excretion. However, when Zn supply was excessive, increasing from 80 to 160 mg Zn/kg, no further changes were seen in Zn absorption, and homeostatic control appeared to be effected entirely by changes in rates of body Zn loss.5. Gel chromatography of intestinal cytosol on Sephadex G-75 revealed that Zn was associated with two major fractions. The first (peak 1) had a molecular weight (MW) > 75 kdaltons and the second (peak 2), a MW of approximately 10 kdaltons and was assumed to be metallothionein.6. There was no obvious relation between the amount of Zn bound to peak 1 and dietary Zn content. In contrast, the amount of Zn recovered in peak 2 increased linearly with increasing dietary Zn content.7. Comparisons between the effect of dietary Zn content on Zn bound to peak 2 and 65Zn retention may, depending on the range of Zn intakes, indicate possible roles for intestinal metallothionein in the control of Zn absorption or excretion.8. A study of the effects of dietary dose of 65Zn on the extent of 65Zn absorption in rats of normal Zn status indicated a possible biphasic relation. At low doses (5–40 mg Zn/kg) 65Zn absorption appeared to exhibit a curvilinear response to increasing 65Zn dose, indicating possibly a saturable process. At higher doses (40–160 mg Zn/kg) the capacity of this process appeared to be exceeded and 65Zn absorption increased in a linear fashion.

Regional geochemical mapping in areas lacking surface drainages: Cu, Fe, Mn, and Zn content of overburden and soil in south-central Saskatchewan

1979 ◽  
Vol 16 (5) ◽  
pp. 1086-1093 ◽  
Author(s):  
P. J. Doyle ◽  
W. K. Fletcher
Keyword(s):  
Statistical Significance ◽  
Parent Material ◽  
Geochemical Mapping ◽  
Parent Materials ◽  
South Central ◽  
Zn Content ◽  
Mean And Variance ◽  
Mean Differences ◽  
Iron Manganese ◽  
Range Test

An approach to regional geochemical mapping is outlined utilizing differences among mean metal concentrations for soil associated with individual parent materials. Initially the area of study is divided into parent material-based subareas for soil collection. Separate mean and variance metal values are estimated for each parent material and Duncan's Multiple Range Test is used to evaluate the statistical significance of among mean differences. Results are summarized in map form showing only compositionally distinctive parent materials or parent material groups and their associated mean and range values.This approach was tested in the Rosetown–Outlook area of south-central Saskatchewan. Results suggest that, in total, fewer than 10 samples are required per parent material to produce stable maps for copper, iron, manganese, and zinc in this 10 000 km2 area. Because of the limited number of samples needed, the method is both rapid and relatively inexpensive. It is therefore ideally suited for regional geochemical surveying, particularly in relatively arid areas where conventional lake and stream sediment-based procedures are not applicable.

scholarly journals An estimate of phytate intake and molar ratio of phytate to zinc in the diet of the people in the United Kingdom

2010 ◽  
Vol 13 (9) ◽  
pp. 1380-1388 ◽  
Author(s):  
F Amirabdollahian ◽  
R Ash
Keyword(s):  
United Kingdom ◽  
Elderly Population ◽  
The Elderly ◽  
Molar Ratio ◽  
Daily Consumption ◽  
Nutrition Survey ◽  
Molar Ratios ◽  
The United Kingdom ◽  
The People ◽  
Phytate Content

AbstractObjectiveTo estimate the phytate intake and molar ratio of phytate to zinc in the diet of the people in the United Kingdom.DesignTables of the phytate content of foods were developed from twenty-eight published and unpublished studies. They were then applied to the nutrient databank of the National Diet and Nutrition Survey (NDNS). The study is a retrospective analysis of data on daily consumption of foods and drinks from the NDNS of children, adolescents, adults and the elderly based on 4–7 d weighed intakes.SubjectsA total of 6786 British participants aged 1·5 years and above, who participated in the NDNS, 1992–2001.SettingEngland, Scotland and Wales.ResultsThe median daily intakes of phytate for children, adolescents, adults and the elderly population were 496, 615, 809 and 629 mg/d, respectively. Although there were differences in phytate intakes between men and women, and for children, adolescents and elderly populations, after adjusting for differences in energy intake, there was no significant variation. The median phytate-to-zinc molar ratios for children, adolescents, adults and the elderly population were 11·8, 10·4, 9·7 and 8·7, respectively. Overall, the main sources of phytate were cereal and cereal products (e.g. breakfast cereals and breads), vegetables, potatoes and savoury snacks (e.g. chips and crisps), hot drinks and miscellaneous foods (e.g. commercial toddler foods and drinks, chocolate and soups), fruits and nuts.ConclusionsThe present study estimated the dietary intake of phytate and the phytate-to-zinc molar ratio of the diet of the UK population, which can be used for estimating the average requirement of zinc. Further research should focus on the completion and validation of the tables of phytate content of UK foods, to assess (and if necessary improve) the accuracy and precision of these findings.

scholarly journals Relationships between Maternal and Fetal Liver Copper, Iron, Manganese, and Zinc Concentrations and Fetal Development in California Holstein Dairy Cows

1994 ◽  
Vol 6 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Thomas W. Graham ◽  
Mark C. Thurmond ◽  
F. Charles Mohr ◽  
Charles A. Holmberg ◽  
Mark L. Anderson ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Trace Element ◽  
Fetal Liver ◽  
Mammalian Species ◽  
Liver Copper ◽  
Trace Element Status ◽  
Zinc Concentrations ◽  
Flame Spectrophotometry ◽  
Iron Manganese ◽  
Holstein Dairy Cows

Associations between maternal trace element deficiencies and abortion have been made for many mammalian species. Objectives of this study were to estimate and correlate maternal and fetal hepatic Cu, Fe, Mn, and Zn concentrations through gestation. Additionally, aborted fetuses, stratified by cause of abortion (infectious or noninfectious), were compared to size-matched nonaborted fetuses to examine for magnitude and direction of change in hepatic trace element status. Dam and fetal liver were removed at slaughter from 103 Holstein dairy cows judged grossly normal by ante- and postmortem examination. Liver samples were collected from fetuses submitted by veterinarians for routine diagnosis of abortion (n = 80). Hepatic Cu, Fe, Mn, and Zn concentrations were determined by flame spectrophotometry. Comparisons of groups, estimations of correlations, and derived prediction equations were made by least-squares methods. Maternal liver Cu, Fe, Mn, and Zn concentrations did not vary during gestation. Compared with the dam, fetal liver Fe and Zn concentrations were higher ( P < 0.05), fetal Cu concentrations were similar ( P > 0.05), and fetal liver Mn concentrations were lower ( P < 0.05). As fetal size increased, fetal liver Cu and Zn concentrations increased (P < 0.05), fetal liver Fe concentration decreased ( P < 0.05), and fetal liver Mn did not change ( P > 0.05). Aborted fetuses had lower liver Cu, Mn, and Zn concentrations than did nonaborted fetuses ( P < 0.05). Liver Fe concentration was lower in aborted fetuses than in nonaborted fetuses in the second trimester only ( P < 0.05). Consistently lower liver Cu, Fe, Mn, and Zn concentrations in aborted fetuses suggest a nonspecific change in trace element status, which implies an effect of abortion, not a cause of abortion.

Net Protein Ratio Data: AACC-ASTM Collaborative Study

1984 ◽  
Vol 67 (2) ◽  
pp. 255-262
Author(s):  
Muriel L Happich ◽  
C E Bodwell ◽  
L Ross Hackler ◽  
John G Phillips ◽  
Philip H Derse ◽  
...  
Keyword(s):  
Vegetable Protein ◽  
Corn Oil ◽  
Collaborative Study ◽  
Feed Consumption ◽  
Peanut Flour ◽  
Test Diet ◽  
Protein Ratio ◽  
Protein Sources ◽  
Textured Vegetable Protein ◽  
Net Protein

Abstract Seven- and 14-day net protein ratio (NPR) data were obtained from 7 laboratories for 6 protein sources: ANRC casein, lean beef, Iactalbumin, textured vegetable protein, and peanut flour were fed as 10% protein (N × 6.25) in the test diet. Wheat flour, casein, and textured vegetable protein were fed as 6% protein (N × 6.25) in the test diet. Weighed dry ingredients for each diet were sent to each collaborator, who mixed the dry ingredients, then added specified amounts of corn oil and water and mixed each complete diet thoroughly. Rats were adapted for 0, 2, or 4 days, and then were fed the test diets for 28 days for protein efficiency ratio (PER) diets. The animal weight gain and feed consumption data obtained after 7 or 14 days of feeding were used to calculate NPR values. Analyses of data were done before [net protein ratio (NPR)] and after (R-NPR [relative-NPR]) adjustment of the data from each laboratory by its results for the reference protein casein. From the analysis of variance for NPR, significant (P &lt;0.05) interactions were observed among laboratories, protein sources, and adaptation times of the animals (0,2, or 4 days). Inter- and intralaboratory variability were decreased by use of 14-day values compared with 7- day values. Adjustment of the NPR data to R-NPR did not lower the intralaboratory variability but did lower the interlaboratory variability of the data. Increasing adaptation time did not consistently decrease interlaboratory or intralaboratory variability or decrease coefficients of variation (CV) of R-NPR values. The 14-day NPR inter- and intralaboratory variations for the 10 % protein diet over all factors (5 protein diets, 3 adaptation periods, and 7 laboratories), as measured by CV values, were 13.2 and 7.7%, respectively. The corresponding R-NPR values were 9.2 and 8.0%, respectively.

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