scholarly journals Nutritive value of mixed proteins

1977 ◽  
Vol 37 (3) ◽  
pp. 309-319 ◽  
Author(s):  
A. A. Woodham ◽  
Eileen M. W. Clarke
Keyword(s):  
Amino Acid ◽  
Essential Amino Acid ◽  
Sunflower Seed ◽  
Geometric Mean ◽  
Soya Bean ◽  
Seed Meal ◽  
Meat Meal ◽  
Bean Meal ◽  
Groundnut Meal ◽  
Soya Bean Meal

1. A fish meal, meat meal, soya-bean meal, groundnut meal and sunflower-seed meal of known amino acid composition were evaluated individually, and combined in all possible pairs, by the estimation of net protein utilization (NPU) and protein efficiency ratio (body-weight gain:crude protein intake; per) using rats. Each pair provided a total of 100 g protein/ kg diet made up so that the amounts of the constituents were (w/w) 100:0, 80:20, 60:40, 20:80 and 0:100.2. Marked synergistic effects were noted only for mixtures of sunflower-seed meal with soya-bean, fish and meat meals.3. Chemical score ([amount of limiting amino acid/the rat's requirement for the same amino acid] × 100; CS), but not essential amino acid index; geometric mean for the ratio, amount of essential amino acid: the rat's requirement for that amino acid, for all ten essential amino acids; EAAI), successfully predicted the rankings of all mixtures except groundnut meal-meat meal and groundnut meal-soya-bean meal, by both per and NPU tests.4. Although there is broad agreement linking results of per and NPU tests with results obtained by a more practical feeding trial in which the mixtures were evaluated as supplements to cereals, neither of these two standard tests is capable of predicting in every instance the advantages to be gained by mixing protein concentrates.

scholarly journals The effect of feeding high levels of low-quality proteins to growing chickens

1975 ◽  
Vol 34 (3) ◽  
pp. 363-373 ◽  
Author(s):  
E. Wetnli ◽  
T. R. Morris ◽  
T. P. Shresta
Keyword(s):  
Growth Rate ◽  
Amino Acid ◽  
Dietary Protein ◽  
Soya Bean ◽  
Maximum Efficiency ◽  
Food Utilization ◽  
The Third ◽  
Bean Meal ◽  
Groundnut Meal ◽  
Soya Bean Meal

1. Three growth trials were done using male broiler chicks. In the first two trials, groundnut meal was used, with and without supplementary methionine and lysine. In the third trial, soya-bean meal was used with and without supplementary methionine. Protein levels ranged in the first trial from 120 to 420 g/kg diet and in the third trial from 120 to 300 g/kg diet. Thus the assumed minimal amino acid requirements of the chick were supplied by high levels of low-quality dietary protein.2. Diets based on cereals and groundnut meal did not support maximum live-weight gain or maximum efficiency of food utilization at any level of dietary protein. When the principal deficiencies of lysine and methionine were corrected, this protein mixture was capable of supporting the same growth rate as a control diet of cereals and herring meal.3. Diets based on maize and soya-bean meal did not support quite the same growth rate as similar diets supplemented with methionine, even though the protein level in the unsupplemented diets was sufficient to meet the assumed methionine requirements.4. These results are interpreted as examples of amino acid imbalance in diets composed of familiar feeding-stuffs. It is concluded that one cannot assume that the poor quality of a protein source can always be offset by increasing the concentration of dietary protein.

Sweet lupin seed meal as a protein source for growing pigs

1975 ◽  
Vol 20 (3) ◽  
pp. 413-419 ◽  
Author(s):  
M. R. Taverner
Keyword(s):  
Growth Performance ◽  
Soya Bean ◽  
Growing Pigs ◽  
Seed Meal ◽  
Food Utilization ◽  
Crude Fibre ◽  
Lupin Seed ◽  
Meat Meal ◽  
Bean Meal ◽  
Soya Bean Meal

SUMMARYThe growth performance, carcass quality and digestive efficiency of growing pigs were used to compare sweet lupin seed meal (SLSM) to soya bean meal and meat meal as a source of supplementary protein to wheat in diets for pigs, and to study the influence of low levels of lysine and methionine, high levels of crude fibre and oil, and possible toxic factors in SLSM on the use of SLSM in such diets.The growth rate and food utilization of pigs between 20 and 80 kg live weight were in the order soya bean meal > SLSM > meat meal, but only the difference between soya bean meal and meat meal was significant. These feeds did not differ significantly in their effects on the proportion of lean in the ham. Adding lysine to the SLSM diet improved growth performance but adding methionine did not. The digestibility of crude fibre in diets containing SLSM was greater than that in diets containing soya bean meal; the digestible energy contents of SLSM and soya bean meal were 4·13±0·05 and 4·16±0·08 Mcal/kg dry matter, respectively. Pigs fed on a diet containing 27·5% SLSM grew faster but utilized food as efficiently when fed ad libitum as on a restricted scale of feeding. The subcutaneous fat of pigs fed on SLSM had a higher iodine number than that of pigs fed on soya bean meal.

A study of the protein and amino acid requirements of the growing New Zealand White rabbit with emphasis on lysine and the sulphur-containing amino acids

1978 ◽  
Vol 39 (3) ◽  
pp. 601-613 ◽  
Author(s):  
D. Spreadbury
Keyword(s):  
Amino Acids ◽  
New Zealand ◽  
Amino Acid ◽  
Dietary Protein ◽  
Fish Meal ◽  
Soya Bean ◽  
N Retention ◽  
Bean Meal ◽  
Groundnut Meal ◽  
Soya Bean Meal

1. New Zealand White (NZW) rabbits were given, between 4 and 8 weeks of age, a range of diets, based on oats and fish meal, containing from 104 to 255 g crude protein (nitrogen × 6.25; CP)/kg to establish the level of CP below which growth was retarded.2. In three experiments each diet was fed to four animals and food intake, growth and N balance were measured over 4 weeks. Body analysis was also carried out after two of the experiments.3. The rates of food intake and growth of animals increased with dietary CP concentration until a CP concentration of approximately 150 g/kg diet had been reached. Beyond this there was little further improvement. N balance studies showed that once this dietary concentration of CP had been reached, there was a reduced rate of N retention.4. Good agreement was found between N retention measured by balance methods and by body analysis: body composition showed a tendency towards an increase in fat and a decrease in N as the dietary protein concentration was reduced.5. Microbial protein produced in the caecum and eaten during coprophagy, was found to supplement the dietary protein by approximately 2 g CP/d, or by only 0.1 of a normal dietary intake of CP.6. In the second part of the study NZW rabbits were offered, between 5 and 8 weeks of age, diets based on oats containing 150 g CP/kg. The protein supplied by oats was supplemented with maize gluten, gelatin, groundnut meal, casein, soya-bean meal or fish meal.7. Rabbits offered diets containing casein, soya-bean meal and fish meal gained 40–50 g/d similar, to animals given a well-balanced control diet, while those given diets containing maize gluten, gelatin or groundnut meal gained approximately 30 g/d. This indicated that amino acid balance in dietary protein was important to the growing rabbit.8. In later experiments, diets based on cereals and groundnut meal supplemented with varying amounts of lysine and methionine were offered during a 3-week post-weaning period in order to assess requirements for those limiting amino acids.9. The addition of both lysine and methionine improved growth rates. The minimum requirements for normal growth were found to be 6.2 g methionine+cystine and 9.4 g lysine/kg diet.

scholarly journals The digestion by cattle of silage-containing diets fed at two dry matter intakes

1985 ◽  
Vol 54 (2) ◽  
pp. 483-492 ◽  
Author(s):  
H. A. Greife ◽  
J. A. Rooke ◽  
D. G. Armstrong
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Dry Matter ◽  
Soya Bean ◽  
Rumen Bacteria ◽  
Bean Meal ◽  
Soya Bean Meal

1. In a 4 x 4 Latin square experiment four cows were given, twice daily, diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 (H) times maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehyde (HCH0)-treated (T).2. The passage of digesta to the duodenum was estimated using chromic oxide as a flow marker;35S was used to estimate the amount of microbial protein entering the small intestine. A microbial fraction was prepared by differential centrifugation from duodenal digesta. Samples of bacteria and of protozoa from rumen digesta were also prepared.3. The total amino acid contents of feedingstuffs, duodenal digesta, duodenal microbial material, rumen bacteria and rumen protozoa were determined by ion-exchange chromatography. The D-alanine and D-glutamic acid contents of the samples were determined by gas–liquid chromatography.4. The quantity of each amino acid entering the small intestine was significantly (P < 0,001) increased by increasing DM intake and tended to be increased by formaldehyde-treatment of the soya-bean meal. There were net losses of all amino acids across the forestomachs except for lysine, methione, o-alanine and D-glutamic acid for which there were net gains.5. There were significant (P < 0.05) differences in amino acid composition between rumen bacteria and duodenal microbial material; differences in amino acid composition between rumen bacteria and rumen protozoa were also observed.6. D-Alanine and D-glutamic acid were present in the silage but not in the barley or either of the soya-bean meals. All samples of microbes and digesta contained D-alanine and D-glutamic acid.7. The use of D-ahine and D-glUtamiC acid as markers for microbial nitrogen entering the small intestine was assessed. Estimates of the quantities of microbial N entering the small intestine based on the D-alanine or D-glutamic acid contents of rumen bacteria or duodenal microbes were significantly higher than those determined using 35S as a marker.

scholarly journals Methionine sulphoxide as a source of sulphur-containing amino acids for the young rat

1977 ◽  
Vol 37 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Anne U. Gjøen ◽  
L. R. Njaa
Keyword(s):  
Amino Acid ◽  
Blood Plasma ◽  
Fish Meal ◽  
Soya Bean ◽  
Male Rats ◽  
Curvilinear Relationship ◽  
Casein Diet ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Effect On Growth

1. Young male rats were used in five experiments to study the utilization for growth of methionine sulphoxide, and the relationship between the sulphoxide content in the diet and the level of microbiologically determined methionine activity in blood or blood plasma. In one nitrogen-balance experiment methionine and methionine sulphoxide were compared as supplements to a casein diet and a fish-meal diet.2. Methionine sulphoxide was poorly utilized for growth when tested as the sole sulphur amino acid in an amino acid diet. Substitution of one-third of the sulphoxide with cystine improved utilization so that it approached that of methionine.3. Methionine alone and in combination with methionine sulphoxide were added to a soya-bean-meal diet. The sulphoxide showed no adverse effect on growth.4. Fish meal in which methionine had been oxidized to methionine sulphoxide was tested alone and in combinations with unoxidized fish meal. Only when the oxidized meal was given alone was there an appreciable effect on growth. The fish meals used were low in cystine.5. Whereas both methionine and methionine sulphoxide improved the N balance when a casein diet was given, there was no effect when a fish-meal diet was given.6. There was a linear relationship between methionine sulphoxide content in the amino acid diets and the methionine activity in the blood plasma. Methionine sulphoxide added to a soya-bean-meal diet or present in oxidized fish meal gave a curvilinear relationship, and the observed activities were lower than with the amino acid diets. Methionine activity in blood could not be used as an indicator of moderate amounts of methionine sulphoxide in protein-containing diets.

Nutrition of the early-weaned calf. IX. Nitrogen retention from different protein sources in calves fed ad libitum

1966 ◽  
Vol 8 (1) ◽  
pp. 43-45 ◽  
Author(s):  
M. Kay ◽  
T. R. Preston ◽  
N. A. MacLeod ◽  
Euphemia B. Philip
Keyword(s):  
Fish Meal ◽  
Nitrogen Retention ◽  
Soya Bean ◽  
Distillers Grains ◽  
Protein Sources ◽  
Dried Distillers Grains ◽  
Bean Meal ◽  
Ad Libitum ◽  
Groundnut Meal ◽  
Soya Bean Meal

1. Nitrogen balance studies were conducted on 8 early-weaned calves fed on four diets containing respectively Peruvian fish meal, soya bean meal, groundnut meal and dried distillers grains as the major sources of protein.2. Nitrogen retention differed significantly between diets, being highest on the fish meal diet, and lowest on the groundnut diet.

Digestibilities of nitrogen and amino acids in soya-bean, sunflower, meat and rapeseed meals measured with pigs and poultry

1989 ◽  
Vol 48 (1) ◽  
pp. 157-179 ◽  
Author(s):  
S. Green ◽  
T. Kiener
Keyword(s):  
Amino Acids ◽  
Growth Response ◽  
Live Weight ◽  
Rapeseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Sunflower Meal ◽  
Meat Meal ◽  
Bean Meal ◽  
Soya Bean Meal

ABSTRACTIn order to determine the relative digestibilities of nitrogen and amino acids in foodstuffs for pigs and poultry, and the effects of manufacturing methods, equal quantities of soya-bean meal, sunflower meals [hulled (sunflower meal 1) and dehulled (sunflower meal 2)], meat meals [made with (meat meal 1), and without (meat meal 2), blood added at 250 g/kg meat tissue (wet weights)] and rapeseed meals [seeds heated at 80°C (rapeseed meal 1) or 100°C (rapeseed meal 2)] were mixed with protein-free ingredients. The diets were given to five growing pigs with ileo-rectal anastomoses, and, by crop-intubation, to 12 caecectomized and 12 intact cocks. Excreta were collected over 48-h periods. Endogenous excretion was estimated by giving protein-free diets.In the order, soya-bean meal, sunflower meals 1, and 2, meat meals 1, and 2, rapeseed meals 1, and 2, true digestibilities were: with pigs, of nitrogen, 0·81, 0·80, 0·79, 0·64, 0·79, 0·73, 0·70 (s.e.d. 0·030), of lysine, 0·84, 0·83, 0·84, 0·65, 0·84, 0·76, 0·72 (s.e.d. 0·032); with caecectomized birds, of nitrogen, 0·92, 0·91, 0·91, 0·66, 0·78, 0·74, 0·75 (s.e.d. 0·018), of lysine 0·92, 0·91, 0·93, 0·62, 0·79, 0·70, 0·70 (s.e.d. 0·020); with intact birds, values were similar to those with caecectomized birds for soya-bean, and the sunflower meals, but lesser for meat meals 1 and 2; the solubilities of nitrogen in pepsin were 0·96, 0·92, 0·93, 0·80, 0·89, 0·87, 0·87.Two hundred and eighty pigs (initial live weights 10 kg) were used to compare growth response to free lysine with that to lysine in soya-bean meal and sunflower meal 2. Lysine availabilities, assessed by analyses of regressions of live-weight gain against lysine intake were 0·82 (s.e. 0·12) for soya-bean meal, and 0·82 (s.e. 0·18) for sunflower meal 2.Amino acid digestibilities of the sunflower meals were similar to those of soya-bean meal, and were not influenced by dehulling; values for the rapeseed meals were lower, and unaffected by differences in heating severity; values for the meat meal were reduced by blood addition. Values differed between pigs and poultry, but there was consistency in the extent to which each species discriminated between some foodstuffs. The pepsin test was insensitive. The large standard errors associated with availability values prevented meaningful comparisons with digestibility values.

scholarly journals The digestion of protein in young pigs and the utilization of dietary methionine

1985 ◽  
Vol 53 (1) ◽  
pp. 137-147 ◽  
Author(s):  
Jane Leibholz
Keyword(s):  
Large Intestine ◽  
Soya Bean ◽  
The Other ◽  
Apparent Digestibility ◽  
Major Site ◽  
Protein Concentrates ◽  
Total Length ◽  
Meat Meal ◽  
Bean Meal ◽  
Soya Bean Meal

1.Forty pigs between 23 and 51 d of age were given ad lib. diets containing wheat and one of five protein concentrates: meat meal A, meat meal B, soya-bean meal, milk and lupins (Lupinus augustifolius). Twenty of these pigs were given indigestible markers from 51 to 56 d of age and were killed at 56 d of age.2.The diets containing meat meals A and B, soya-bean meal and milk contained 2.3 g total methionine/kg and the diet containing lupins contained 2.1 g/kg.3.A further forty pigs of the same age were given the same diets supplemented with 1 g synthetic methionine/kg.4.The weight gains and feed conversion ratios of the pigs given the diets containing 2.1–2.3 g methionine and 3.1–3.3 g methionine/kg were not significantly different.5. The weight gains of the pigs given lupins (2.1 g methionine/kg) were less than those of the pigs given the diets containing 2.3 g methionine/kg.6. The apparent digestibility of dry matter (DM) and nitrogen was less for the diets containing the meat meals (0.75 and 0.78 respectively) than for those containing the other protein concentrates (0.80 and 0.84).7.The retention times in the large intestine of the diets containing soya-bean meal and lupins were 965 and 1083 min which were greater than those of the diets containing the other protein concentrates, mean 732 min.8. The major site of N digestion and absorption for the diet containing milk was the area of the small intestine 25–50% of total length from the pylorus, while for the other protein concentrates the major site was 50–75% of its total length from the pylorus.9. The digestion and absorption of N in the large intestine was less (3.4%) for the diet containing milk than for those containing the other protein concentrates (7.5–11.3%).10. The apparent digestibility of the methionine to the ileum for the five diets ranged from 0.74 to 0.86 while the calculated retention of the apparently-absorbed methionine was 1.00. It was suggested that methionine digestibility could be used as an indicator of availability.11. The calculated retention of apparently absorbed N in the carcass was 0.71 for the pigs given the diet containing milk and 0.51–0.58 for the pigs given the other diets.

Total amino acid requirements of weaned piglets 8 to 25 kg live weight given diets based on wheat and soya-bean meal fortified with free amino acids

1992 ◽  
Vol 54 (2) ◽  
pp. 281-287 ◽  
Author(s):  
F. Gatel ◽  
G. Buron ◽  
J. Fékéte
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Amino Acid Content ◽  
Live Weight ◽  
Maximum Growth ◽  
Soya Bean ◽  
Weaned Piglets ◽  
Bean Meal ◽  
Soya Bean Meal

AbstractTwo experiments were carried out with weaned piglets from 8 to 25 kg live weight in order to determine the dietary amino acid content necessary for maximum growth. Six diets based on wheat, soya-bean meal, soya-bean oil and free amino acids were compared in each experiment. Essential amino acids were in the same relative proportion for all diets: (methionine + cystine)/lysine = 0·60 to 0·65; threonine/lysine = 0·65; tryptophan/lysine = 0·19. The range of amino acid content was 9·53 to 12·52 g lysine per kg in the first experiment and 11·34 to 15·94 g lysine per kg in the second experiment. The number of piglets used per diet was 136 (20 pens) and 106 (16 pens) in respectively the first and the second experiment. The relationship between either dietary lysine content or daily lysine intake and growth rate was quadratic and significant. Dietary lysine content and daily lysine intake which enable maximum growth were calculated according to this model. Dietary lysine contents were 15·5 and 14·9 g/kg for the first 3 weeks (8 to 17 kg) and for the overall post-weaning period (8 to 25 kg) respectively. Daily lysine intakes were 10·6 and 13·3 g/day respectively for the same two periods. Reasons for these values being higher than those currently cited are discussed.

Estimation of endogenous amino acid losses in growing chickens given soya-bean meal supplemented or not with DL-methionine

2002 ◽  
Vol 75 (3) ◽  
pp. 415-426 ◽  
Author(s):  
I. Fernández-Fígares ◽  
R. Nieto ◽  
the late C. Prieto ◽  
J. F. Aguilera
Keyword(s):  
Body Weight ◽  
Regression Analysis ◽  
Amino Acid ◽  
Live Weight ◽  
Soya Bean ◽  
Metabolizable Energy ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Protein Free Diet ◽  
Lower Ileum

AbstractAn experiment was carried out in growing chickens to study the effect of supplementation of a semi-synthetic diet containing soya-bean meal as the sole protein source with DL-methionine, to improve its biological value, on the excretion of endogenous protein and amino acids measured in lower ileum and total tract using traditional methods. Thirty-two White Rock male broilers (10 days old) were randomly divided into eight groups each of four birds, of similar body weight (mean live weight: 142·8 (s.e. 0·68) g), and individually housed in metabolism cages. Following a paired-feeding design based on metabolic body weight (kgM0·75), each group of birds was given, for an experimental period of 20 days, each of four levels of protein (60, 120, 180 or 240 g/kg; 5 days each) in two groups of isoenergetic (14·5 kJ metabolizable energy per g dry matter) and semi-synthetic diets based on soya-bean meal, either not supplemented or supplemented with 2 g/kg DL-methionine (diets S and SM, respectively). After 3 days of each treatment excreta were collected for 48 h, frozen and stored at –20ºC. At the end of the fourth treatment three chickens of each group were killed and their lower ileal contents collected. The remaining chick of each treatment was fasted for 24 h and given a protein-free diet for 8 days and excreta were collected for the last 4 days. Then (day 39 of age), chickens were killed and lower ileum contents removed and stored at –20ºC. Samples of excreta and lower ileum contents were subjected to nitrogen (N) analysis by Kjeldahl procedure and amino acid (AA) analysis by high-performance liquid chromatography. Supplementation with DL-methionine of the soya-bean meal-based diets halved total tract endogenous AA losses. Regression analysis produced a higher estimation of ileal and faecal endogenous AA excretion than feeding a protein-free diet. Endogenous AA excretion determined in the lower ileum was higher than in excreta no matter which estimation procedure was utilized. In conclusion, supplementation of dietary protein with the first limiting AA to improve its protein quality, causes an important drop in endogenous AA losses, that may have an important effect on the N economy and energy requirements in poultry. The use of regression analysis on excreta data where graded amounts of protein are given to growing chickens, seems a suitable method for determining endogenous AA losses provided that good quality proteins are used.

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