scholarly journals Nutritional evaluation of the protein of dried tomato pomace in the rat

1976 ◽  
Vol 36 (3) ◽  
pp. 449-456 ◽  
Author(s):  
N. J. Drouliscos

1. Nutritional evaluation of dried tomato pomace (DTP-20) as a source of protein was carried out using weanling rats. Comparisons were made with casein (CS), soya-bean meal (SOM-45) and the hydrocarbon-grown yeast Toprina (BP-T). The growth-promoting effects of the diets were evaluated over a period of 28 d ofad lib.feeding.2. The unsupplemented DPT-20 had a protein efficiency ratio (per) of 2.18±0.13 and a net protein utilization (npu) of 0.55. The addition ofdlmethionine (5 g/kg) resulted in aperof 1.74±0.15 (t2.99,P< 0.01) and anpuof 0.40, while the addition of an amino acid mixture resulted in aperof 2.33±0.08 (t1.84,P< 0.1) and anpuof 0.70.3. The reason for the decrease in growth and the reducedperandnpuvalues recorded for the methionine-supplemented group of rats is not clear and it is discussed in connexion with an imbalance or a slight toxicity caused by the added amino acid.

1980 ◽  
Vol 43 (1) ◽  
pp. 115-123 ◽  
Author(s):  
N. J. Drouliscos ◽  
Vastliki Malefaki

1. Evaluation of the germ meal (CGM) of carob seed (Ceratonia siliqua) and its protein isolate was carried out with weanling rats. Comparisons were made with casein, soya-bean meal, whole defatted egg and a soya-bean protein isolate (Promine-D) as protein sources. The growth-promoting effects and certain biological indices were evaluated using the protein efficiency ratio (PER), biological value (BV) and net protein utilization (NPU) bioassay procedures.2. The unsupplemented CGM had a PER of 1.66 ± 0.09 and an NPU of 0.58 ± 0.013. Addition of DL-methionine at 4, 8 and 12 g/kg diet resulted in a PER of 1.95 ± 0.11, 2.01 ± 0.11 and 1.90 ± 0.11 respectively. The corresponding BV values were 0.80 ± 0.003, 0.78 ± 0.015 and 0.74 ± 0.011, and those for NPU 0.69 ± 0.013, 0.66 ± 0.026 and 0.63 ± 0.020 respectively. The addition of amino acids improved the PER (2.24–2.59), BV (0.78–0.79) and NPU (0.71–0.73) values.3. The BV and NPU assays for the unsupplemented carob germ isolate were low (BV 0.36 ± 0.016, NPU 0.35 ± 0.015). Supplementation with amino acids resulted in a positive increase with values of 0.66 ± 0.013 and 0.64 ± 0.013 for BV and NPU respectively.


1977 ◽  
Vol 55 (3) ◽  
pp. 434-438 ◽  
Author(s):  
Christian A. Stephens ◽  
Margaret J. Veen-Baigent ◽  
Alenka Paquet ◽  
G. Harvey Anderson

α-N,ε-N-Dipalmitoyl-L-lysine, α-N-monopalmitoyl-L-lysine, and ε-N-monopalmitoyl-L-lysine were compared with 18% casein and lysine HCl as sources of lysine in the diets of weanling rats. The lysine derivatives and lysine HCl were added to a basal diet containing 14.1% of an amino acid mixture with no lysine. Weight gain, food intake, plasma lysine, and urine and fecal lysine were determined. Animals fed the basal and lysine derivative diets lost weight over the 10-day study period. They had lower food intake, urine and plasma lysine content, and a higher fecal lysine content than animals fed the other diets. α-N-Monopalmitoyl-L-lysine was absorbed whereas the ε-N-monopalmitoyl-L-lysine and the α-N,ε-N-dipalmitoyl compounds were not.


Nutrition ◽  
2020 ◽  
Vol 69 ◽  
pp. 110588 ◽  
Author(s):  
Francesco Bellanti ◽  
Aurelio Lo Buglio ◽  
Elena Di Stasio ◽  
Giorgia di Bello ◽  
Rosanna Tamborra ◽  
...  

1970 ◽  
Vol 100 (3) ◽  
pp. 380-380 ◽  
Author(s):  
Hans Fisher

2015 ◽  
Vol 114 (11) ◽  
pp. 1845-1851 ◽  
Author(s):  
Yean Yean Soong ◽  
Joseph Lim ◽  
Lijuan Sun ◽  
Christiani Jeyakumar Henry

AbstractConsumption of high glycaemic index (GI) and glycaemic response (GR) food such as white rice has been implicated in the development of type 2 diabetes. Previous studies have reported the ability of individual amino acids to reduce GR of carbohydrate-rich foods. Because of the bitter flavour of amino acids, they have rarely been used to reduce GR. We now report the use of a palatable, preformed amino acid mixture in the form of essence of chicken. In all, sixteen healthy male Chinese were served 68 or 136 ml amino acid mixture together with rice, or 15 or 30 min before consumption of white rice. Postprandial blood glucose and plasma insulin concentrations were measured at fasting and every 15 min after consumption of the meal until 60 min after the consumption of the white rice. Subsequent blood samples were taken at 30-min intervals until 210 min. The co-ingestion of 68 ml of amino acid mixture with white rice produced the best results in reducing the peak blood glucose and GR of white rice without increasing the insulinaemic response. It is postulated that amino acid mixtures prime β-cell insulin secretion and peripheral tissue uptake of glucose. The use of ready-to-drink amino acid mixtures may be a useful strategy for lowering the high-GI rice diets consumed in Asia.


1985 ◽  
Vol 54 (2) ◽  
pp. 483-492 ◽  
Author(s):  
H. A. Greife ◽  
J. A. Rooke ◽  
D. G. Armstrong

1. In a 4 x 4 Latin square experiment four cows were given, twice daily, diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 (H) times maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehyde (HCH0)-treated (T).2. The passage of digesta to the duodenum was estimated using chromic oxide as a flow marker;35S was used to estimate the amount of microbial protein entering the small intestine. A microbial fraction was prepared by differential centrifugation from duodenal digesta. Samples of bacteria and of protozoa from rumen digesta were also prepared.3. The total amino acid contents of feedingstuffs, duodenal digesta, duodenal microbial material, rumen bacteria and rumen protozoa were determined by ion-exchange chromatography. The D-alanine and D-glutamic acid contents of the samples were determined by gas–liquid chromatography.4. The quantity of each amino acid entering the small intestine was significantly (P < 0,001) increased by increasing DM intake and tended to be increased by formaldehyde-treatment of the soya-bean meal. There were net losses of all amino acids across the forestomachs except for lysine, methione, o-alanine and D-glutamic acid for which there were net gains.5. There were significant (P < 0.05) differences in amino acid composition between rumen bacteria and duodenal microbial material; differences in amino acid composition between rumen bacteria and rumen protozoa were also observed.6. D-Alanine and D-glutamic acid were present in the silage but not in the barley or either of the soya-bean meals. All samples of microbes and digesta contained D-alanine and D-glutamic acid.7. The use of D-ahine and D-glUtamiC acid as markers for microbial nitrogen entering the small intestine was assessed. Estimates of the quantities of microbial N entering the small intestine based on the D-alanine or D-glutamic acid contents of rumen bacteria or duodenal microbes were significantly higher than those determined using 35S as a marker.


1975 ◽  
Vol 34 (3) ◽  
pp. 363-373 ◽  
Author(s):  
E. Wetnli ◽  
T. R. Morris ◽  
T. P. Shresta

1. Three growth trials were done using male broiler chicks. In the first two trials, groundnut meal was used, with and without supplementary methionine and lysine. In the third trial, soya-bean meal was used with and without supplementary methionine. Protein levels ranged in the first trial from 120 to 420 g/kg diet and in the third trial from 120 to 300 g/kg diet. Thus the assumed minimal amino acid requirements of the chick were supplied by high levels of low-quality dietary protein.2. Diets based on cereals and groundnut meal did not support maximum live-weight gain or maximum efficiency of food utilization at any level of dietary protein. When the principal deficiencies of lysine and methionine were corrected, this protein mixture was capable of supporting the same growth rate as a control diet of cereals and herring meal.3. Diets based on maize and soya-bean meal did not support quite the same growth rate as similar diets supplemented with methionine, even though the protein level in the unsupplemented diets was sufficient to meet the assumed methionine requirements.4. These results are interpreted as examples of amino acid imbalance in diets composed of familiar feeding-stuffs. It is concluded that one cannot assume that the poor quality of a protein source can always be offset by increasing the concentration of dietary protein.


1977 ◽  
Vol 37 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Anne U. Gjøen ◽  
L. R. Njaa

1. Young male rats were used in five experiments to study the utilization for growth of methionine sulphoxide, and the relationship between the sulphoxide content in the diet and the level of microbiologically determined methionine activity in blood or blood plasma. In one nitrogen-balance experiment methionine and methionine sulphoxide were compared as supplements to a casein diet and a fish-meal diet.2. Methionine sulphoxide was poorly utilized for growth when tested as the sole sulphur amino acid in an amino acid diet. Substitution of one-third of the sulphoxide with cystine improved utilization so that it approached that of methionine.3. Methionine alone and in combination with methionine sulphoxide were added to a soya-bean-meal diet. The sulphoxide showed no adverse effect on growth.4. Fish meal in which methionine had been oxidized to methionine sulphoxide was tested alone and in combinations with unoxidized fish meal. Only when the oxidized meal was given alone was there an appreciable effect on growth. The fish meals used were low in cystine.5. Whereas both methionine and methionine sulphoxide improved the N balance when a casein diet was given, there was no effect when a fish-meal diet was given.6. There was a linear relationship between methionine sulphoxide content in the amino acid diets and the methionine activity in the blood plasma. Methionine sulphoxide added to a soya-bean-meal diet or present in oxidized fish meal gave a curvilinear relationship, and the observed activities were lower than with the amino acid diets. Methionine activity in blood could not be used as an indicator of moderate amounts of methionine sulphoxide in protein-containing diets.


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