scholarly journals A collaborative test of ion-exchange chromatographic methods for determining amino acids

1968 ◽  
Vol 22 (3) ◽  
pp. 437-450 ◽  
Author(s):  
J. W. G. Porter ◽  
D. R. Westgarth ◽  
A. P. Williams
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Chromatographic Methods ◽  
Collaborative Test

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 7. ISOLATION OF AMINO ACIDS FROM SERUM AND OTHER FLUIDS

1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Ion Exchange ◽  
Paper Chromatography ◽  
Biological Fluids ◽  
Inorganic Cations ◽  
Chromatographic Methods ◽  
Tissue Extracts

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.

QUANTITATIVE CHROMATOGRAPHIC METHODS FOR THE STUDY OF ENZYMIC TRANSPEPTIDATION REACTIONS

1955 ◽  
Vol 33 (1) ◽  
pp. 416-427 ◽  
Author(s):  
George E. Connell ◽  
Gordon H. Dixon ◽  
Charles S. Hanes
Keyword(s):  
Amino Acids ◽  
Filter Paper ◽  
Quantitative Estimation ◽  
Chromatographic Methods

A colorimetric ninhydrin method has been applied to the quantitative estimation of amino acids and peptides on paper chromatograms. This method is suitable for the analysis of enzymic transpeptidation reactions. A procedure for the purification of filter paper for chromatography has been developed.

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