QUANTITATIVE CHROMATOGRAPHIC METHODS FOR THE STUDY OF ENZYMIC TRANSPEPTIDATION REACTIONS

1955 ◽  
Vol 33 (1) ◽  
pp. 416-427 ◽  
Author(s):  
George E. Connell ◽  
Gordon H. Dixon ◽  
Charles S. Hanes
Keyword(s):  
Amino Acids ◽  
Filter Paper ◽  
Quantitative Estimation ◽  
Chromatographic Methods

A colorimetric ninhydrin method has been applied to the quantitative estimation of amino acids and peptides on paper chromatograms. This method is suitable for the analysis of enzymic transpeptidation reactions. A procedure for the purification of filter paper for chromatography has been developed.

QUANTITATIVE CHROMATOGRAPHIC METHODS FOR THE STUDY OF ENZYMIC TRANSPEPTIDATION REACTIONS

1955 ◽  
Vol 33 (3) ◽  
pp. 416-427 ◽  
Author(s):  
George E. Connell ◽  
Gordon H. Dixon ◽  
Charles S. Hanes
Keyword(s):  
Amino Acids ◽  
Filter Paper ◽  
Quantitative Estimation ◽  
Chromatographic Methods

A colorimetric ninhydrin method has been applied to the quantitative estimation of amino acids and peptides on paper chromatograms. This method is suitable for the analysis of enzymic transpeptidation reactions. A procedure for the purification of filter paper for chromatography has been developed.

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 5. AN IMPROVED NINHYDRIN–HYDRINDANTIN REAGENT

1961 ◽  
Vol 39 (2) ◽  
pp. 417-425 ◽  
Author(s):  
A. T. Matheson ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Determination ◽  
Filter Paper ◽  
High Stability ◽  
Reagent Blank ◽  
Chromatographic Methods ◽  
Ruhemann’S Purple

An improved ninhydrin–hydrindantin reagent has been developed for the quantitative determination of amino acids and peptides separated on filter paper chromatograms. This represents a modification of a reagent described earlier, which was based in turn upon the well-known solution of Moore and Stein. The present reagent has been in use for several years and has shown the advantages of high stability, extremely low and steady reagent blank values, and approximately stoichiometric yields of Ruhemann's purple for most amino acids. In addition to its use for determinations on excised areas from filter paper chromatograms, conditions are defined for the use of the reagent for determinations of amino acids in solutions.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

Stability of amino acids and galactose in the newborn screening filter paper blood specimen

1985 ◽  
Vol 107 (5) ◽  
pp. 757-760 ◽  
Author(s):  
Harvey L. Levy ◽  
Jane R. Simmons ◽  
Robert A. MacCready
Keyword(s):  
Amino Acids ◽  
Newborn Screening ◽  
Filter Paper ◽  
Blood Specimen

Microbiological Test for Leucine, Valine, and Isoleucine Using Urine Sample Dried on Filter Paper

1962 ◽  
Vol 8 (3) ◽  
pp. 242-245 ◽  
Author(s):  
Helen K Berry ◽  
Carolyn Scheel ◽  
Joy Marks
Keyword(s):  
Amino Acids ◽  
Early Detection ◽  
Filter Paper ◽  
Microbiological Test ◽  
The Third ◽  
Maple Sugar ◽  
Urine Disease ◽  
Screening Procedures ◽  
Mentally Retarded Children ◽  
Urine Specimens

Abstract Urine specimens dried on filter paper squares were obtained from 700 mentally retarded children. A microbiological assay was used to screen the specimens for the presence of valine, leucine, and isoleucine. Three specimens gave positive tests for all three amino acids. A generalized aminoaciduria was observed in two specimens, and in the third, elevation of valine and leucine/isoleucine was confirmed by paper chromatography. The use of these screening procedures for early detection of children with maple sugar urine disease is suggested.

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 7. ISOLATION OF AMINO ACIDS FROM SERUM AND OTHER FLUIDS

1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Ion Exchange ◽  
Paper Chromatography ◽  
Biological Fluids ◽  
Inorganic Cations ◽  
Chromatographic Methods ◽  
Tissue Extracts

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.

scholarly journals The Effect of Mineral Nutrition on the Content of Free Amino Acids and Amides in Tomato Plants I A Comparison of the Effects of Deficiencies of Copper, Zinc, Manganese, Iron, and Molybdenum

1956 ◽  
Vol 9 (4) ◽  
pp. 539 ◽  
Author(s):  
JV Possingham
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Mineral Nutrition ◽  
Free Amino Acids ◽  
Free Amino ◽  
Quantitative Estimation ◽  
Acid Fraction ◽  
Tomato Plants ◽  
Copper Zinc

The lovel and the qllHnj~itat,jve compositien of the free amino acid fraction of tomato plants grown in full nutrient and in cultures doficiollt ill zinc, copper, nlallganeso, iron. and molybdenum have beon cletormiuecl. 'I'he methods used in the investigation inelude the quantitative estimation of amino aeids by a technique involving paper chromatogl'l1phy, and the cultm'o of tomato plants in highly purified n utriollt solutions.

scholarly journals Electrophoretic Separation on an Origami Paper-Based Analytical Device Using a Portable Power Bank

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1724
Author(s):  
Yu Matsuda ◽  
Katsunori Sakai ◽  
Hiroki Yamaguchi ◽  
Tomohide Niimi
Keyword(s):  
Amino Acids ◽  
Filter Paper ◽  
Type A ◽  
Separation Distance ◽  
Electrophoretic Separation ◽  
Ph Distribution ◽  
Portable Power ◽  
Paper Device ◽  
Analytical Device

The electrophoresis of ampholytes such as amino acids on a paper device is difficult because of the variation of pH distribution in time. On the basis of this observation, we propose a paper-based analytical device (PAD) with origami structure. By folding a filter paper, a low operation voltage of 5 V was achieved, where the power was supplied by a 5 V 1.5 A portable power bank through the USB type A receptacle. As a demonstration, we carried out the electrophoretic separation of pI markers (pI 5.5 and 8.7). The separation was achieved within 4 min before the pH distribution on the paper varied. Though the separation distance was small, it could be increased by expanding the origami structure. This result indicates that our proposed PAD is useful for electrophoretic separation on a paper device.

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