Effects of microwave irradiation on ruminal degradation and in vitro digestibility of soya-bean meal

2005 ◽  
Vol 80 (3) ◽  
pp. 369-375 ◽  
Author(s):  
A. A. Sadeghi ◽  
A. Nikkhah ◽  
P. Shawrang
Keyword(s):  
Sodium Dodecyl ◽  
Soya Bean ◽  
Water Soluble ◽  
In Vitro Digestibility ◽  
Α Subunit ◽  
Sds Page ◽  
Ruminal Degradation ◽  
Bean Meal ◽  
Soya Bean Meal

AbstractThis study was carried out to determine ruminal dry matter (DM) and crude protein (CP) degradation characteristics of untreated, 2-, 4- and 6-min microwave-treated soya-bean meal (SBM) by using nylon bags and sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE) techniques. Nylon bags of untreated or treated SBM were suspended into the rumen of three Holstein steers from 0 to 48 h, and data were fitted to non-linear degradation characteristics to calculate effective rumen degradation (ERD). There were significant differences (P < 0·05) for DM and CP degradation parameters between untreated and microwave-treated SBM. Microwave treatments decreased the water-soluble fraction and increased the potentially degradable fraction of CP. The degradation rate of the latter fraction decreased with these treatments. As a consequence, microwave treatments decreased (P < 0·05) ERD of CP. From densitometric scanning, SBM proteins were seen to be composed of two major components; β-conglycinin and glycinin, accounting for proportionately 0·30 and 0·40 of buffer-soluble SBM proteins, respectively. Electrophoretic analysis of untreated, 2-, 4- and 6-min microwave-treated SBM protein residues revealed that two of the subunits of β-conglycinin (α -and α) were degraded completely after 2, 4, 24 and 48 h, respectively, whereas the α subunit of this protein was more resistant to degradation. In untreated SBM, the two subunits of glycinin (acidic and basic polypeptides) were degraded in the middle of the incubation period, but in microwave-treated SBM were not degraded until 48 h of incubation. In vitro digestibility of ruminally undegraded CP of untreated and treated SBM increased (P < 0·05) with increases in rumen incubation time from 8 to 24 h. In conclusion, SBM proteins appeared to be effectively protected from ruminal degradation by a 4-min microwave treatment. SDS-PAGE results indicated that ruminally undegraded protein from untreated SBM was mainly composed of the basic subunit of glycinin, whereas that from microwave-treated SBM was composed of β-conglycinin and both basic and acidic subunits of glycinin.

scholarly journals PSVII-35 Late-Breaking Abstract: Microwave heat treatment feed effect on ruminal degradability and intestinal protein digestibility - a brief review

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 342-343
Author(s):  
Md Safiqur Rahaman Shishir ◽  
Muhammad Jamal Khan ◽  
Hassan Khanaki ◽  
Graham Brodie ◽  
Brendan Cullen ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Protein Digestibility ◽  
Soya Bean ◽  
Canola Meal ◽  
Canola Seed ◽  
Intestinal Protein ◽  
Bean Meal ◽  
Ruminal Degradability ◽  
Soya Bean Meal

Abstract Rumen degradability of crude protein (CP) of feed is a major factor that determines the utilization of CP in ruminant production. This study briefly reviewed the findings from six international studies of microwave (MW) heat treatment effect on feed CP rumen degradability and intestinal CP digestibility. Six in vitro studies of concentrate feed (canola seed, canola meal, soya bean meal, cottonseed meal, corn, and barley) showed a decrease in effective rumen degradability of dry matter and protein by 4–40% and 17–40%, respectively compared to control group (untreated concentrate feed). Among the six studies, four studies identified the MW heat treatment effect on intestinal protein digestibility. Due to MW heat treatment, canola seed, canola meal, soya bean meal, and cottonseed meal showed an increase in intestinal CP digestibility by 17%, 20%, 21%, and 19%, respectively. Overall the briefly reviewed studies showed that, MW heat treatment substantially reduced feed CP ruminal degradability and increased in vitro CP digestibility of ruminally undegraded CP.

The use of a novel in vitro technique for the assessment of the ruminal degradation of soya bean meal.

1993 ◽  
Vol 1993 ◽  
pp. 167-167
Author(s):  
A T Chamberlain
Keyword(s):  
Pore Size ◽  
Cold Water ◽  
Latin Square ◽  
Soya Bean ◽  
Base Line ◽  
Artificial Fibre ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
In Sacco

The metabolisabie protein system (AFRC, 1992) requires the measurement of the dynamics of the degradation of protein in the rumen. However the recommended method, using fistulated animals, is slow, expensive and may be considered unacceptable by many in terms of animal welfare. The objective of this work was to develop an in vitro technique for the routine assessment of feed protein degradability.A 101 vessel fitted with an automatic stirrer was filled with modified van Soest media and rumen liquor (pooled from two sheep) and maintained under anaerobic conditions at 39.5°C. Small artificial fibre bags (4.5x3cm, pore size 54 micro-m ±4) were filled with 0.4g of soya bean meal and incubated in the vessel for 3,6,10 and 24 hours using 3 replicates at each time. Bags were washed in running cold water for 30 mins immediately after removal. Four replicates of 3g samples in large Dacron bags (10x7cm, pore size 55 micro-m ±22) were also incubated in sacco in fistulated yearling wethers using a 4x4 Latin square design to assess the base line degradability. In sacco bags were rinsed and frozen after removal and washed in a domestic washing machine at the end of the experiment.

Effects of the addition of starch on the utilization by sheep of the protein and crude fibre contained in lucerne hay and soya-bean meal

1971 ◽  
Vol 77 (3) ◽  
pp. 413-425 ◽  
Author(s):  
H. Tagari ◽  
D. Ben Gedalya ◽  
Y. Shevach ◽  
A. Bondi
Keyword(s):  
Energy Content ◽  
Energy Requirement ◽  
Soya Bean ◽  
Soluble Carbohydrates ◽  
Control Groups ◽  
Protein Requirement ◽  
Crude Fibre ◽  
Bean Meal ◽  
Soya Bean Meal

SUMMARYA sheep-feeding experiment was carried out to compare the influence of starch supplementation on the utilization of protein contained in a roughage (lucerne hay) and in a concentrated feed (soya-bean meal). Twelve young rams were divided into four groups, all of which received 150% of the theoretical protein requirement for maintenance. Two groups received soya-bean meal, and the two other groups were given alfalfa hay as protein source. One soya-bean group and one lucerne hay group served as control groups and received the theoretical requirement of energy, whereas the energy content of both experimental groups was raised by addition of grain to 150% of the theoretical energy requirement.A N-balance experiment carried out with both control groups did not indicate the superiority of lucerne hay over soya-bean meal as had been found in previous experiments in which the sheep were fed diets just supplying the theoretical protein requirement. The advantage of roughage protein disappeared as a result of changes of dietary regime, such as the ingestion of protein surpluses and the reduction in the amount of cottonseed hulls.The N retention from the lucerne-hay ration was enhanced much more than the soya-bean meal ration by the addition of maize. Biochemical analyses of the rumen oontent showed that a much higher metabolic activity of the micro-organisms was induced by feeding a lucerne-hay diet supplying 150% of the theoretical protein requirement than that induced by ingestion of a corresponding amount of soya-bean meal. It was concluded that the increased biochemical potential of the lucerne-hay diet could be used advantageously only in presence of ample amounts of soluble carbohydrates. The digestibility of the carbohydrates contained in all four diets was examined by in vivo and in vitro methods. The digestibility of crude fibre in the lucerne-hay ration was somewhat raised by the addition of maize whereas the digestibility of crude fibre in the soya-bean meal ration was considerably depressed by the addition of maize.The digestibility of the carbohydrates contained in all four diets was examined by in vivo and in vitro methods. The digestibility of crude fibre in the lucerne-hay ration was somewhat raised by the addition of maize whereas the digestibility of crude fibre in the soya-bean meal ration was considerably depressed by the addition of maize.

scholarly journals Net absorption from portal-drained viscera of nitrogenous compounds by beef heifers fed on diets differing in protein solubility or degradability in the rumen

1987 ◽  
Vol 57 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Gerald B. Huntington
Keyword(s):  
Blood Meal ◽  
Protein Solubility ◽  
Latin Square ◽  
Soya Bean ◽  
Beef Heifers ◽  
Nitrogenous Compounds ◽  
Dietary Nitrogen ◽  
Bean Meal ◽  
Soya Bean Meal

1. The objective of the present study was to evaluate effects of in vitro rumen solubility or degradability of dietary protein on net absorption of nitrogenous compounds from portal-drained viscera of beef heifers.2. Four protein sources, casein, soya-bean meal, maize-gluten meal and blood meal provided about two-thirds of total dietary nitrogen in semi-purified diets given to four beef heifers in a 4 x 4 Latin square design.3. Although in vitro degradability of dietary N ranged from 842 (casein) to 310 (blood meal) g/kg total dietary N, net absorption of ammonia-N or α-amino-N was not significantly different (P > 0.10) among diets. However, net absorption of NH3-N tended to decrease and net absorption of α-amino-N tended to increase as intake of in vitro undegradable N increased. Net transfer of urea-N from plasma to portal-drained viscera was greater (P < 0.10) when heifers were fed on soya-bean meal than when fed on maize-gluten meal or blood meal.4. The findings show with all diets that the non-protein-nitrogenous compounds, NH3 and urea, played a substantial role in absorption and transfer of N. Overall, net absorption of NH3-N was 61% of net absorption of α-amino-N, and transfer of urea-N from plasma to portal-drained viscera was 80% ofnet absorption of α-amino-N.

scholarly journals IN-VITRO DIGESTIBILITTY OF BARLEY GRAINS TREATED WITH UREA: IN-VITRO STUDY

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Tawfeeq & et al.
Keyword(s):  
Nitrogen Content ◽  
Total Nitrogen ◽  
In Vitro Study ◽  
Barley Grain ◽  
Total Nitrogen Content ◽  
In Vitro Digestibility ◽  
Bean Meal ◽  
Barley Grains ◽  
Soya Bean Meal

This study was conducted in a central laboratory of Agriculture College\ University of Baghdad, to study the effect of treated barley grains with urea as 1: 1 urea: barley grain (w:w) with or without heating on the in-vitro digestibility, total nitrogen content and releasing urea, the results showed a high significant increases (P<0.01) in total nitrogen content of treated barley grains with urea compared with non-treated, where it was 14.77% for barley soaked 24 hours with urea and 17.18% for barley- urea treated with heat and pressure in compared to 2.15% for untreated barley grains. There were continuity in urea production from barley grains soaked with urea for most times 0, 10, 20, 30, 60,120,180, and 240 minutes and a highly significant (P<0.01) compared to barley grains treated with urea. There were no significant differences in the in-vitro digestibility for concentrated rations when substituted treated barley grains with soya bean meal. In conclusion: we can use barley grains soaked with urea for ruminant feeding without side effects in contrast direct feeding of urea.

Ruminal degradability of soya bean meal treated with different doses of tannic acid

1997 ◽  
Vol 1997 ◽  
pp. 123-123
Author(s):  
E. Serrano ◽  
M. S. Safigueroa ◽  
J. Giráldez ◽  
A.R. Mantecón
Keyword(s):  
Tannic Acid ◽  
Dietary Protein ◽  
Soya Bean ◽  
Ruminal Degradation ◽  
Bean Meal ◽  
Ruminal Degradability ◽  
Chemical Conditions ◽  
Soya Bean Meal ◽  
Physical And Chemical ◽  
Different Doses

It is well known that tannins are able to form complexes with dietary protein that can protect protein against ruminal degradation (Driedger 1972, Mangan 1988). However, level of protection could change with factors such as nature and amount of tannins and physical and chemical conditions in the ruminal fluid. The present experiment was conducted to study the ruminal degradability under different dietary conditions of soya bean meal sprayed witht different amounts of tannic acid.

Effect of supplementary crude protein level and degradability in grass silage-based diets on performance of dairy cows, and digestibility and abomasal nitrogen flow in sheep

1990 ◽  
Vol 51 (2) ◽  
pp. 235-244 ◽  
Author(s):  
R. F. Cody ◽  
J. J. Murphy ◽  
D. J. Morgan
Keyword(s):  
Crude Protein ◽  
Fish Meal ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Soya Bean ◽  
Grass Silage ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Daily Intakes

ABSTRACTFour concentrate supplements differing in crude protein (CP) and undegradable protein (UDP) content were offered to 16 lactating Friesian cows together with grass silage (dry matter (DM) 196 g/kg, pH 4·38, CP 160 g/kg DM, in vitro DM digestibility 0·68)ad libitumin a Latin-square trial with 3-week periods. The supplement treatments were: (1) barley 122 g CP per kg DM, degradability (dg) 0·77; (2) barley/soya-bean meal 210 g CP per kg DM, dg 0·69; (3) barley/soya-bean meal/fish meal 190 g CP per kg DM, dg 0·61; (4) barley/soya-bean meal/fish meal 219 g CP per kg DM, dg 0·59. Supplements were given at 8 kg/day. Total daily intakes of silage (kg DM), CP and UDP (g) on treatments 1 to 4 were 7·77, 2087, 375; 8·35, 2804, 655; 8·29, 2676, 717; 8·70, 2917, 826, respectively. Milk yield (kg/day) and yields of fat, protein and lactose (g/day) on the four treatments were 21·3, 791, 617, 984; 23·0, 816, 688, 1055; 23·0, 818, 696, 1050; 23·6, 813, 735, 1071 for treatments 1 to 4 respectively. Yield and concentration of protein and lactose were significantly lower on treatment 1 than on the other treatments, while the of blood metabolites indicated treatment effects on blood glucose, non-esterified fatty acids, plasma protein and urea. Digestibility of organic matter and non-ammonia nitrogen (NAN) flow to the abomasum (g/day), measured in sheep given a fixed silage/supplement ratio at maintenance, were 0·81 and 18·4, 0·81 and 20·8, 0·82 and 21·4, 0·82 and 22·4 for treatments 1 to 4 respectively. The NAN flow was significantly greater on treatment 4 than on treatment 1.

In vitro and in vivo rumen protection of proteins coated with calcium soaps of long-chain fatty acids

1989 ◽  
Vol 112 (1) ◽  
pp. 79-83 ◽  
Author(s):  
D. Sklan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Soya Bean ◽  
Fatty Acid Production ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
In Sacco

SummaryThe in sacco, in vitro and in vivo effects of feeding proteins partially coated with calcium soaps of longchain fatty acids were examined.In sacco, 84–90% of whey powder and soya-bean meal coated with calcium salts of fatty acids remained after 20 h incubation in the rumen of sheep. In vitro tests revealed no effects on volatile fatty acid or ammonia production.In vivo sheep balance studies, where soya-bean meal coated with calcium soaps was substituted for soya-bean meal, showed no effects on ammonia or volatile fatty acid production in the rumen due to the calcium soap coated proteins. No changes were observed in digestibilities of dry matter, nitrogen or acid detergent fibre; total fatty acid digestion increased. Nitrogen balance was improved slightly in sheep fed the protected protein.It appears that proteins coated with calcium soaps are not degraded in the rumen and thus energy and non-degradable protein can be supplied to ruminants by this route.

scholarly journals Rumen protein degradation rates estimated by non-linear regression analysis of Michaelis–Menten in vitro data

1992 ◽  
Vol 67 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Glen A. Broderick ◽  
Murray K. Clayton
Keyword(s):  
Linear Regression ◽  
Protein Degradation ◽  
Degradation Rate ◽  
Soya Bean ◽  
Protein Sources ◽  
Degradation Rates ◽  
Non Linear ◽  
Bean Meal ◽  
Soya Bean Meal

An in vitro method applying Michaelis–Menten saturation kinetics was developed as an alternative approach for estimating protein degradation rates in the rumen. Non-linear regression (NLR) analysis of the integrated Michaelis–Menten equation yielded fractional degradation rates,kd, from direct estimates of the maximum velocity: Michaelis constant ratio (kd=Vmax:Km). Degradation rates obtained using data from a series of 2 h inhibitor in vitro incubations were respectively 0.989, 0.134, and 0.037 /h for casein, solvent soya-bean meal (SSBM) and expeller soya-bean meal (ESBM). Degradation rates obtained from 2 h incubations had lower standard errors than those obtained using 1 h incubations; 2 h rates were not significantly different from 1 h rates, suggesting end-product inhibition was not significant at 2 h. The NLR Michaelis–Menten method was used to determine degradation rates for twelve protein sources: casein, bovine serum albumin, two samples of lucerne (Medicago sativa) hay, and four samples each of SSBM and ESBM. Statistical analysis of NLR results revealed significant differences among the twelve protein sources. Casein was degraded most rapidly (0.827 /h), and the four ESBM samples most slowly (0.050–0.098 /h). Degradation rate for serum albumin was 0.135 /h; rates for SSBM and lucerne hays ranged from 0.160 to 0.208 /h. Degradation rates estimated using the NLR method were more rapid than those obtained with a limited substrate approach; NLR rates were more consistent with in vivo estimates of rumen protein escape. Greater concentrations of slowly degraded proteins were needed with the NLR method to define curvilinearity of the degradation curve more accurately.Protein degradation rate: Rumen protein escape: Michaelis–Menten kinetics: Non-linear regression

scholarly journals The effect of giving feeds containing soya-bean meal treated or extracted with ethanol on digestive processes in the prerurninant calf

1979 ◽  
Vol 42 (3) ◽  
pp. 477-485 ◽  
Author(s):  
J. W. Sissons ◽  
R. H. Smith ◽  
D. Hewitt
Keyword(s):  
Nutritive Value ◽  
Aqueous Ethanol ◽  
N Uptake ◽  
Soya Bean ◽  
Water Soluble ◽  
Bean Flour ◽  
Weanling Rats ◽  
Bean Meal ◽  
Toxic Constituent ◽  
Soya Bean Meal

1. Preruminant calves, fitted with abomasal and re-entrant ileal cannulas, were given, at intervals of 2–3 d by infusion into the absomasum, a series of five single experimental feeds containing heated soyabean flour (product B) as the only protein source. The calves were sensitized in this way to a constituent in the soya beans and by the fifth feed showed a number of digestive disturbances. Further test feeds were then given in which heated soya-bean flour was sometimes replaced by soya-bean products prepared under laboratory or commercial conditions by treating I part fat-free raw soya-bean meal with either I part (product M) or 4 parts (product L) ethanol (750 ml/l) at 78–80° and evaporating the whole mixture to dryness, or by extracting I part meal with 4 parts aqueous ethanol under similar conditions (product K). Products M, L and K were heated with steam and finely ground. Feeds prepared from casein and from commerical ethanol-extracted concentrate (product D) were also examined.2. Measurements were made of transit time through the small intestine, flow rate of ileal digesta, recovery of polyethylene glycol (a water-soluble marker added to the feed) and net nitrogen absorption up to the distal ileum. Compared to feeds based on casein, feeds containing products K, L or M showed some differences in digesta movement and N uptake, but much less disturbance than feeds containing product B. Products prepared by extracting soya-bean meal with ethanol appeared to be slightly superior to those prepared from meal treated with ethanol, but the differences were not significant.3. Weanling rats were used to assess the nutritive value of soya-bean products by an N balance method. Digestibilities (mean 0.948) and biological values (mean 0.860) obtained for products B, D, K and L did not differ significantly.4. Results confirmed that extracting soya-bean meal with hot aqueous ethanol improved its value for calf feeding and indicated that at least part of the effect was due to the destruction of a toxic constituent present in the soya bean which probably acted by inducing a gastrointestinal allergy.

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