scholarly journals Structural and Biochemical Characterization of the Type II Fructose-1,6-bisphosphatase GlpX fromEscherichia coli

2008 ◽  
Vol 284 (6) ◽  
pp. 3784-3792 ◽  
Author(s):  
Greg Brown ◽  
Alexander Singer ◽  
Vladimir V. Lunin ◽  
Michael Proudfoot ◽  
Tatiana Skarina ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Type Ii ◽  
Fromescherichia Coli ◽  
Fructose 1,6 Bisphosphatase

Biochemical characterization of a novel type-II VEGFR2 kinase inhibitor: Comparison of binding to non-phosphorylated and phosphorylated VEGFR2

2011 ◽  
Vol 19 (18) ◽  
pp. 5342-5351 ◽  
Author(s):  
Hidehisa Iwata ◽  
Shinichi Imamura ◽  
Akira Hori ◽  
Mark S. Hixon ◽  
Hiroyuki Kimura ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Kinase Inhibitor ◽  
Type Ii

scholarly journals Molecular and Biochemical Characterization of a Distinct Type of Fructose-1,6-Bisphosphatase from Pyrococcus furiosus

2002 ◽  
Vol 184 (12) ◽  
pp. 3401-3405 ◽  
Author(s):  
Corné H. Verhees ◽  
Jasper Akerboom ◽  
Emile Schiltz ◽  
Willem M. de Vos ◽  
John van der Oost
Keyword(s):  
Biochemical Characterization ◽  
Pyrococcus Furiosus ◽  
Distinct Type ◽  
Sequence Motifs ◽  
Conserved Sequence ◽  
New Family ◽  
Fructose 1,6 Bisphosphatase ◽  
Conserved Sequence Motifs ◽  
Fructose 1,6 Bisphosphate

ABSTRACT The Pyrococcus furiosus fbpA gene was cloned and expressed in Escherichia coli, and the fructose-1,6-bisphosphatase produced was subsequently purified and characterized. The dimeric enzyme showed a preference for fructose-1,6-bisphosphate, with a Km of 0.32 mM and a V max of 12.2 U/mg. The P. furiosus fructose-1,6-bisphosphatase was strongly inhibited by Li+ (50% inhibitory concentration, 1 mM). Based on the presence of conserved sequence motifs and the substrate specificity of the P. furiosus fructose-1,6-bisphosphatase, we propose that this enzyme belongs to a new family, class IV fructose-1,6-bisphosphatase.

scholarly journals Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidiumEimeria tenella(PhylumApicomplexa)

2007 ◽  
Vol 272 (2) ◽  
pp. 238-244 ◽  
Author(s):  
Xiaomin Cai ◽  
A. Lorraine Fuller ◽  
Larry R. McDougald ◽  
Xiangshi Tan ◽  
Jianping Cai ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Biochemical Characterization ◽  
Acid Synthesis ◽  
Type Ii ◽  
Enoyl Reductase
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