Biochemical characterization of a novel type-II VEGFR2 kinase inhibitor: Comparison of binding to non-phosphorylated and phosphorylated VEGFR2

2011 ◽  
Vol 19 (18) ◽  
pp. 5342-5351 ◽  
Author(s):  
Hidehisa Iwata ◽  
Shinichi Imamura ◽  
Akira Hori ◽  
Mark S. Hixon ◽  
Hiroyuki Kimura ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Kinase Inhibitor ◽  
Type Ii

scholarly journals Structural and Biochemical Characterization of the Type II Fructose-1,6-bisphosphatase GlpX fromEscherichia coli

2008 ◽  
Vol 284 (6) ◽  
pp. 3784-3792 ◽  
Author(s):  
Greg Brown ◽  
Alexander Singer ◽  
Vladimir V. Lunin ◽  
Michael Proudfoot ◽  
Tatiana Skarina ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Type Ii ◽  
Fromescherichia Coli ◽  
Fructose 1,6 Bisphosphatase

scholarly journals Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidiumEimeria tenella(PhylumApicomplexa)

2007 ◽  
Vol 272 (2) ◽  
pp. 238-244 ◽  
Author(s):  
Xiaomin Cai ◽  
A. Lorraine Fuller ◽  
Larry R. McDougald ◽  
Xiangshi Tan ◽  
Jianping Cai ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Biochemical Characterization ◽  
Acid Synthesis ◽  
Type Ii ◽  
Enoyl Reductase

Purification and partial biochemical characterization of a membrane-bound type II-like α-glucosidase from the yeast morphotype of Sporothrix schenckii

2011 ◽  
Vol 101 (2) ◽  
pp. 313-322 ◽  
Author(s):  
Blanca I. Torres-Rodríguez ◽  
Karina Flores-Berrout ◽  
Julio C. Villagómez-Castro ◽  
Everardo López-Romero
Keyword(s):  
Biochemical Characterization ◽  
Sporothrix Schenckii ◽  
Type Ii ◽  
Membrane Bound

scholarly journals Biochemical and structural characterization of the BioZ enzyme engaged in bacterial biotin synthesis pathway

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sitao Zhang ◽  
Yongchang Xu ◽  
Hongxin Guan ◽  
Tao Cui ◽  
Yuling Liao ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Biochemical Characterization ◽  
Acid Synthesis ◽  
Catalytic Triad ◽  
Type Ii ◽  
Protein Phylogeny ◽  
Domains Of Life ◽  
Fas Ii

AbstractBiotin is an essential micro-nutrient across the three domains of life. The paradigm earlier step of biotin synthesis denotes “BioC-BioH” pathway in Escherichia coli. Here we report that BioZ bypasses the canonical route to begin biotin synthesis. In addition to its origin of Rhizobiales, protein phylogeny infers that BioZ is domesticated to gain an atypical role of β-ketoacyl-ACP synthase III. Genetic and biochemical characterization demonstrates that BioZ catalyzes the condensation of glutaryl-CoA (or ACP) with malonyl-ACP to give 5’-keto-pimeloyl ACP. This intermediate proceeds via type II fatty acid synthesis (FAS II) pathway, to initiate the formation of pimeloyl-ACP, a precursor of biotin synthesis. To further explore molecular basis of BioZ activity, we determine the crystal structure of Agrobacterium tumefaciens BioZ at 1.99 Å, of which the catalytic triad and the substrate-loading tunnel are functionally defined. In particular, we localize that three residues (S84, R147, and S287) at the distant bottom of the tunnel might neutralize the charge of free C-carboxyl group of the primer glutaryl-CoA. Taken together, this study provides molecular insights into the BioZ biotin synthesis pathway.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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