scholarly journals The Gα12/13 Family of Heterotrimeric G Proteins and the Small GTPase RhoA Link the Kaposi Sarcoma-associated Herpes Virus G Protein-coupled Receptor to Heme Oxygenase-1 Expression and Tumorigenesis

2007 ◽  
Vol 282 (47) ◽  
pp. 34510-34524 ◽  
Author(s):  
MarÁa José MartÁn ◽  
Tamara Tanos ◽  
Ana Belén GarcÁa ◽  
Daniel Martin ◽  
J. Silvio Gutkind ◽  
...  
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Heme Oxygenase ◽  
Kaposi Sarcoma ◽  
Small Gtpase ◽  
Heme Oxygenase 1 ◽  
Heterotrimeric G Proteins ◽  
G Protein Coupled Receptor ◽  
Herpès Virus ◽  
G Protein Coupled

scholarly journals Inhibition of Heme Oxygenase-1 Interferes with the Transforming Activity of the Kaposi Sarcoma Herpesvirusencoded G Protein-coupled Receptor

2006 ◽  
Vol 281 (16) ◽  
pp. 11332-11346 ◽  
Author(s):  
Maria Julia Marinissen ◽  
Tamara Tanos ◽  
Marta Bolós ◽  
Maria Rosa de Sagarra ◽  
Omar A. Coso ◽  
...  
Keyword(s):  
G Protein ◽  
Heme Oxygenase ◽  
Kaposi Sarcoma ◽  
Heme Oxygenase 1 ◽  
G Protein Coupled Receptor ◽  
Transforming Activity ◽  
G Protein Coupled

NFκB pathway is down-regulated by 1α,25(OH)2-vitamin D3 in endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein coupled receptor

Steroids ◽  
2012 ◽  
Vol 77 (11) ◽  
pp. 1025-1032 ◽  
Author(s):  
Verónica Gonzalez-Pardo ◽  
Noelia D’Elia ◽  
Annemieke Verstuyf ◽  
Ricardo Boland ◽  
Ana Russo de Boland
Keyword(s):  
Endothelial Cells ◽  
G Protein ◽  
Vitamin D3 ◽  
Herpes Virus ◽  
Kaposi Sarcoma ◽  
G Protein Coupled Receptor ◽  
Herpès Virus ◽  
Nfκb Pathway ◽  
G Protein Coupled

scholarly journals YGLF motif in the Kaposi sarcoma herpes virus G-protein-coupled receptor adjusts NF-κB activation and paracrine actions

Oncogene ◽  
2013 ◽  
Vol 33 (49) ◽  
pp. 5609-5618 ◽  
Author(s):  
S Azzi ◽  
S S Smith ◽  
J Dwyer ◽  
H M Leclair ◽  
C Alexia ◽  
...  
Keyword(s):  
G Protein ◽  
Herpes Virus ◽  
Kaposi Sarcoma ◽  
G Protein Coupled Receptor ◽  
Kaposi Sarcoma Herpes Virus ◽  
Herpès Virus ◽  
Paracrine Actions ◽  
G Protein Coupled

scholarly journals Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors

2021 ◽  
Author(s):  
Hannes Schihada ◽  
Rawan Shekhani ◽  
Gunnar Schulte
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Mammalian Cells ◽  
Living Cells ◽  
Optical Biosensors ◽  
Heterotrimeric G Proteins ◽  
G Protein Coupled Receptor ◽  
Cellular Expression ◽  
G Protein Coupled ◽  
Constitutively Active

AbstractHeterotrimeric G proteins constitute the primary transducers of G protein-coupled receptor (GPCR) signaling. Besides mediating ligand-induced GPCR activation, G proteins transduce basal levels of activity in various physiological and pathophysiological settings evoked by constitutively active, native GPCRs or disease-related receptor mutants. Several generations of optical biosensors were developed and optimized to monitor GPCR ligand-induced G protein activation, however, quantitative approaches to detect constitutively active GPCRs are not available. Here, we designed and validated a set of eight bioluminescence-resonance-energy-transfer (BRET)-based G protein sensors, covering all four major families of G proteins, and established a protocol to identify constitutive GPCR/G protein signaling in living cells. These sensors rely on the encoding of all three G protein subunits on a single plasmid, enabling their cellular expression at desired relative levels and resulting in reduced signal variability in mammalian cells. Based on this sensor platform, we further present here an experimental protocol to quantify constitutive signaling of native and mutated GPCRs through these heterotrimeric transducers. This approach will aid in the characterization of constitutively active GPCRs and the exploration of their role in health and disease.One Sentence SummaryThis Resource article describes the validation of a biophysical approach to directly assess the constitutive signaling activity of G protein-coupled receptors through heterotrimeric G proteins in living cells using optical biosensors.

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