scholarly journals Inhibition of Heme Oxygenase-1 Interferes with the Transforming Activity of the Kaposi Sarcoma Herpesvirusencoded G Protein-coupled Receptor

2006 ◽  
Vol 281 (16) ◽  
pp. 11332-11346 ◽  
Author(s):  
Maria Julia Marinissen ◽  
Tamara Tanos ◽  
Marta Bolós ◽  
Maria Rosa de Sagarra ◽  
Omar A. Coso ◽  
...  
Keyword(s):  
G Protein ◽  
Heme Oxygenase ◽  
Kaposi Sarcoma ◽  
Heme Oxygenase 1 ◽  
G Protein Coupled Receptor ◽  
Transforming Activity ◽  
G Protein Coupled

NFκB pathway is down-regulated by 1α,25(OH)2-vitamin D3 in endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein coupled receptor

Steroids ◽  
2012 ◽  
Vol 77 (11) ◽  
pp. 1025-1032 ◽  
Author(s):  
Verónica Gonzalez-Pardo ◽  
Noelia D’Elia ◽  
Annemieke Verstuyf ◽  
Ricardo Boland ◽  
Ana Russo de Boland
Keyword(s):  
Endothelial Cells ◽  
G Protein ◽  
Vitamin D3 ◽  
Herpes Virus ◽  
Kaposi Sarcoma ◽  
G Protein Coupled Receptor ◽  
Herpès Virus ◽  
Nfκb Pathway ◽  
G Protein Coupled

scholarly journals YGLF motif in the Kaposi sarcoma herpes virus G-protein-coupled receptor adjusts NF-κB activation and paracrine actions

Oncogene ◽  
2013 ◽  
Vol 33 (49) ◽  
pp. 5609-5618 ◽  
Author(s):  
S Azzi ◽  
S S Smith ◽  
J Dwyer ◽  
H M Leclair ◽  
C Alexia ◽  
...  
Keyword(s):  
G Protein ◽  
Herpes Virus ◽  
Kaposi Sarcoma ◽  
G Protein Coupled Receptor ◽  
Kaposi Sarcoma Herpes Virus ◽  
Herpès Virus ◽  
Paracrine Actions ◽  
G Protein Coupled

scholarly journals 1α,25-Dihydroxyvitamin D3 and Its TX527 Analog Inhibit the Growth of Endothelial Cells Transformed by Kaposi Sarcoma-Associated Herpes Virus G Protein-Coupled Receptor in Vitro and in Vivo

Endocrinology ◽  
2010 ◽  
Vol 151 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Verónica Gonzalez-Pardo ◽  
Daniel Martin ◽  
J. Silvio Gutkind ◽  
Annemieke Verstuyf ◽  
Roger Bouillon ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
G Protein ◽  
Kaposi Sarcoma ◽  
Time Dependent ◽  
G Protein Coupled Receptor ◽  
Dihydroxyvitamin D3 ◽  
Antiproliferative Effects ◽  
G Protein Coupled

Abstract The Kaposi sarcoma-associated herpes virus-G protein-coupled receptor is a key molecule in the pathogenesis of Kaposi sarcoma, playing a central role in promoting vascular endothelial growth factor-driven angiogenesis and spindle cell proliferation. We studied the effects of 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] and the analog TX527 on the proliferation of endothelial cells (SVECs) and SVECs transformed by the viral G protein-coupled receptor (SVEC-vGPCR). 1α,25(OH)2D3 and TX527 decreased SVEC-vGPCR and SVEC numbers, the response being time dependent and similar in both cell lines. Vitamin D receptor (VDR) levels increased on treatment with 10 nm 1α,25(OH)2D3 or 1 nm TX527 in a time-dependent manner (1.5–24 h) in SVECs and SVEC-vGPCR. Basal VDR levels were increased in SVEC-vGPCR. The antiproliferative effects were accompanied by reduction in cyclin D1 and accumulation of p27 in SVECs but not SVEC-vGPCR. Induction of VDR was blocked by transfection of short hairpin RNA against VDR in SVEC-vGPCR and the antiproliferative effects of 1α,25(OH)2D3 and TX527 were decreased, involving the VDR genomic pathway in the hormone and analog mechanism of action. In vivo experiments showed that 1α,25(OH)2D3 and TX527 decreased SVEC-vGPCR tumor progression when the tumor cells were implanted in nude mice. In conclusion, we have demonstrated that 1α,25(OH)2D3 and its TX527 analog have antiproliferative effects on the growth of endothelial cells transformed by the vGPCR in vitro and in vivo, the vitamin D receptor being part of the inhibitory mechanism of action.

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