scholarly journals Characterization of Mitochondrial and Extra-mitochondrial Oxygen Consuming Reactions in Human Hematopoietic Stem Cells

2005 ◽  
Vol 280 (28) ◽  
pp. 26467-26476 ◽  
Author(s):  
Claudia Piccoli ◽  
Roberto Ria ◽  
Rosella Scrima ◽  
Olga Cela ◽  
Annamaria D'Aprile ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem

Functional isolation and characterization of human hematopoietic stem cells

Science ◽  
1995 ◽  
Vol 267 (5194) ◽  
pp. 104-108 ◽  
Author(s):  
A. Berardi ◽  
A Wang ◽  
J. Levine ◽  
P Lopez ◽  
D. Scadden
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Isolation And Characterization

scholarly journals Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells

Oncogene ◽  
2001 ◽  
Vol 20 (57) ◽  
pp. 8249-8257 ◽  
Author(s):  
Ken Ohmine ◽  
Jun Ota ◽  
Masuzu Ueda ◽  
Shu-ichi Ueno ◽  
Koji Yoshida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chronic Myeloid Leukemia ◽  
Hematopoietic Stem Cells ◽  
Dna Microarray ◽  
Myeloid Leukemia ◽  
Hematopoietic Stem ◽  
Stage Progression

Characterization of a novel decellularized bone marrow scaffold as an inductive environment for hematopoietic stem cells

2019 ◽  
Vol 7 (4) ◽  
pp. 1516-1528 ◽  
Author(s):  
Juares E. Romero Bianco ◽  
Renata Giardini Rosa ◽  
Ada Congrains-Castillo ◽  
Paulo P. Joazeiro ◽  
Stephen D. Waldman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Bovine Bone ◽  
Hematopoietic Stem ◽  
Bone Marrow Study ◽  
Increasing Demand

Due to the increasing demand for a bone marrow study model, we developed a natural scaffold from decellularized bovine bone marrow (DeBM).

Purification and characterization of mouse hematopoietic stem cells

Science ◽  
1988 ◽  
Vol 241 (4861) ◽  
pp. 58-62 ◽  
Author(s):  
G. Spangrude ◽  
S Heimfeld ◽  
I. Weissman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Purification And Characterization ◽  
Hematopoietic Stem

scholarly journals Physical characterization of hematopoietic stem cells using multidirectional label-free light scatterings

2016 ◽  
Vol 24 (25) ◽  
pp. 28877 ◽  
Author(s):  
Hesam Shahin ◽  
Manisha Gupta ◽  
Anna Janowska-Wieczorek ◽  
Wojciech Rozmus ◽  
Ying Y. Tsui
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Physical Characterization ◽  
Label Free ◽  
Hematopoietic Stem

Functional characterization of hematopoietic stem cells in the spleen

2011 ◽  
Vol 39 (3) ◽  
pp. 351-359.e3 ◽  
Author(s):  
Yohei Morita ◽  
Akiko Iseki ◽  
Satoshi Okamura ◽  
Sachie Suzuki ◽  
Hiromitsu Nakauchi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Functional Characterization ◽  
Hematopoietic Stem

scholarly journals Molecular cloning of a novel receptor tyrosine kinase gene, STK, derived from enriched hematopoietic stem cells

Blood ◽  
1994 ◽  
Vol 83 (11) ◽  
pp. 3160-3169 ◽  
Author(s):  
A Iwama ◽  
K Okano ◽  
T Sudo ◽  
Y Matsuda ◽  
T Suda
Keyword(s):  
Stem Cells ◽  
Tyrosine Kinase ◽  
Hematopoietic Stem Cells ◽  
Tyrosine Kinases ◽  
Growth Regulation ◽  
Chromosome 9 ◽  
Cdna Clones ◽  
Hematopoietic Stem ◽  
Degenerate Oligonucleotide

To identify the novel receptor tyrosine kinases (RTKs) critical to the proliferation of hematopoietic stem cells, we performed polymerase chain reaction-based cloning from highly purified murine hematopoietic stem cells. Lineage marker-negative, c-KIT-positive, and Ly6A/E- or Sca- 1-positive (Lin-c-KIT+Sca-1+) cells were sorted by a fluorescence- activated cell sorter. Two sets of degenerate oligonucleotide primers were directed to the conserved sequences of the catalytic domain, and were used to amplify cDNAs that encode protein tyrosine kinases (PTKs). One hundred cDNA clones were sequenced and 8 RTKs were identified, as well as 12 non-RTKs and 2 serine/threonine kinases. Sixteen cDNAs were identical to the known kinase genes (PKC beta, JAK-1, JAK-2, TYK-2, HCK, FGR, FYN, BLK, c-FES, FER, c-ABL, c-KIT, FLK-1, FLK-2, IGF1R, and ECK). Six novel cDNA sequences (stk series) were identified. However, three of them turned out to be BPK, RYK, and TEK. The remaining three showed high homology to S6 kinase II, JAK-2, and v-SEA/c-MET, respectively. Characterization of full-length cDNA sequence of the v- SEA/cMET-related gene showed that this was a novel RTK gene and we named this gene STK (stem cell-derived tyrosine kinase). We identified two distinct forms of STK cDNA; the short one encoded a putative truncated protein that lacked most of the extracellular domain. STK was expressed at various stages of hematopoietic cells, including stem cells, but we could not detect any apparent expression in other adult tissues. The expression of the truncated form of mRNA was more predominant than that of the complete form. STK was assigned by fluorescent in situ hybridization to the R-positive F1 band of chromosome 9, the same region to which hepatic growth factor-like protein has been assigned. Characterization of these PTKs, including STK, will be helpful to elucidate the molecular mechanism of the growth regulation of hematopoietic stem cells.

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