scholarly journals Bimodal Effect of Advanced Glycation End Products on Mesangial Cell Proliferation Is Mediated by Neutral Ceramidase Regulation and Endogenous Sphingolipids

2004 ◽  
Vol 279 (33) ◽  
pp. 34343-34352 ◽  
Author(s):  
Karen Geoffroy ◽  
Nicolas Wiernsperger ◽  
Michel Lagarde ◽  
Samer El Bawab
Keyword(s):  
Cell Proliferation ◽  
Advanced Glycation End Products ◽  
Mesangial Cell ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Mesangial Cell Proliferation

Morroniside and loganin extracted from Cornus officinalis have protective effects on rat mesangial cell proliferation exposed to advanced glycation end products by preventing oxidative stress

2006 ◽  
Vol 84 (12) ◽  
pp. 1267-1273 ◽  
Author(s):  
Huiqin Xu ◽  
Jian Shen ◽  
Hong Liu ◽  
Yan Shi ◽  
Lihua Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Advanced Glycation End Products ◽  
Mesangial Cell ◽  
Morphological Changes ◽  
Flow Cytometric Analysis ◽  
Advanced Glycation ◽  
Early Stages ◽  
Cornus Officinalis ◽  
Glycation End Products ◽  
End Products

Advanced glycation end products (AGE) are involved in the alterations of renal mesangial cell (MCs) growth, a feature of early stages of diabetic nephropathy (DN). We postulate that morroniside and loganin, 2 components extracted from Cornus officinalis , may ameliorate the detrimental effects of AGE-induced MCs proliferation by preventing oxidative stress. Rat MCs cultured in AGE milieu were treated with morroniside and loganin. Results showed that morroniside and loganin inhibited AGE-induced MC proliferation as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Fluorescence microscopy revealed that the morroniside and loganin improved the morphological changes of MCs. Flow cytometric analysis showed that morroniside and loganin inhibited the cell cycle of rat MCs. Furthermore, the level of reactive oxygen species was significantly reduced, and the activities of superoxide dismutase and glutathione peroxidase were markedly increased, whereas the level of malondialdehyde was not significantly reduced. These results suggest that morroniside and loganin regulate MC growth by preventing oxidative stress. Thus, this study provides a molecular basis for the use of morroniside and loganin in the early stages of DN.

Sign in / Sign up

Export Citation Format

Share Document