scholarly journals High Level Oxacillin and Vancomycin Resistance and Altered Cell Wall Composition inStaphylococcus aureusCarrying the StaphylococcalmecAand the EnterococcalvanAGene Complex

2003 ◽  
Vol 279 (5) ◽  
pp. 3398-3407 ◽  
Author(s):  
Anatoly Severin ◽  
Keiko Tabei ◽  
Fred Tenover ◽  
Marilyn Chung ◽  
Nancy Clarke ◽  
...  
Author(s):  
Eliza Louback ◽  
Diego Silva Batista ◽  
Tiago Augusto Rodrigues Pereira ◽  
Talita Cristina Mamedes-Rodrigues ◽  
Tatiane Dulcineia Silva ◽  
...  

Author(s):  
Margalida Roig‐Oliver ◽  
Catherine Rayon ◽  
Romain Roulard ◽  
François Fournet ◽  
Josefina Bota ◽  
...  

2004 ◽  
Vol 48 (12) ◽  
pp. 4566-4573 ◽  
Author(s):  
Anatoly Severin ◽  
Shang Wei Wu ◽  
Keiko Tabei ◽  
Alexander Tomasz

ABSTRACT A combination of biochemical and genetic experiments were performed in order to better understand the mechanism of expression of high-level vancomycin resistance in Staphylococcus aureus. The transcription of pbp2 of the highly vancomycin- and oxacillin-resistant strain COLVA200 and its mutant derivative with inactivated mecA were put under the control of an inducible promoter, and the dependence of oxacillin and vancomycin resistance and cell wall composition on the concentration of the isopropyl-β-d-thiogalactopyranoside inducer was determined. The results indicate that mecA—the genetic determinant of oxacillin resistance—while essential for oxacillin resistance, is not involved with the expression of vancomycin resistance. Penicillin binding protein 2A, the protein product of mecA, appears to be unable to utilize the depsipeptide cell wall precursor produced in the vancomycin-resistant cells for transpeptidation. The key penicillin binding protein essential for vancomycin resistance and for the synthesis of the abnormally structured cell walls characteristic of vancomycin-resistant S. aureus (A. Severin, K. Tabei, F. Tenover, M. Chung, N. Clarke, and A. Tomasz, J. Biol. Chem. 279:3398-3407, 2004) is penicillin binding protein 2.


2011 ◽  
Vol 81 (1) ◽  
pp. 157-178 ◽  
Author(s):  
Paola Bisicchia ◽  
Nhat Khai Bui ◽  
Christine Aldridge ◽  
Waldemar Vollmer ◽  
Kevin M. Devine

2017 ◽  
Vol 175 (1) ◽  
pp. 259-271 ◽  
Author(s):  
Brwa Rasool ◽  
Jack McGowan ◽  
Daria Pastok ◽  
Sue E. Marcus ◽  
Jenny A. Morris ◽  
...  

2012 ◽  
Vol 10 (9) ◽  
pp. 1077-1087 ◽  
Author(s):  
Alex Y.-L. Tsai ◽  
Thomas Canam ◽  
András Gorzsás ◽  
Ewa J. Mellerowicz ◽  
Malcolm M. Campbell ◽  
...  

2006 ◽  
Vol 72 (10) ◽  
pp. 6483-6492 ◽  
Author(s):  
Jürgen Behr ◽  
Michael G. Gänzle ◽  
Rudi F. Vogel

ABSTRACT Resistance to hops is a prerequisite for lactic acid bacteria to spoil beer. In this study we analyzed mechanisms of hop resistance of Lactobacillus brevis at the metabolism, membrane physiology, and cell wall composition levels. The beer-spoiling organism L. brevis TMW 1.465 was adapted to high concentrations of hop compounds and compared to a nonadapted strain. Upon adaptation to hops the metabolism changed to minimize ethanol stress. Fructose was used predominantly as a carbon source by the nonadapted strain but served as an electron acceptor upon adaptation to hops, with concomitant formation of acetate instead of ethanol. Furthermore, hop adaptation resulted in higher levels of lipoteichoic acids (LTA) incorporated into the cell wall and altered composition and fluidity of the cytoplasmic membrane. The putative transport protein HitA and enzymes of the arginine deiminase pathway were overexpressed upon hop adaptation. HorA was not expressed, and the transport of hop compounds from the membrane to the extracellular space did not account for increased resistance to hops upon adaptation. Accordingly, hop resistance is a multifactorial dynamic property, which can develop during adaptation. During hop adaptation, arginine catabolism contributes to energy and generation of the proton motive force until a small fraction of the population has established structural improvements. This acquired hop resistance is energy independent and involves an altered cell wall composition. LTA shields the organism from accompanying stresses and provides a reservoir of divalent cations, which are otherwise scarce as a result of their complexation by hop acids. Some of the mechanisms involved in hop resistance overlap with mechanisms of pH resistance and ethanol tolerance and as a result enable beer spoilage by L. brevis.


2004 ◽  
Vol 40 (6) ◽  
pp. 968-978 ◽  
Author(s):  
John P. Vogel ◽  
Theodore K. Raab ◽  
Chris R. Somerville ◽  
Shauna C. Somerville

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