scholarly journals Cytoplasmic Compartmentation of Gln3 during Nitrogen Catabolite Repression and the Mechanism of Its Nuclear Localization during Carbon Starvation inSaccharomyces cerevisiae

2002 ◽  
Vol 277 (40) ◽  
pp. 37559-37566 ◽  
Author(s):  
Kathleen H. Cox ◽  
Jennifer J. Tate ◽  
Terrance G. Cooper
Keyword(s):  
Nuclear Localization ◽  
Catabolite Repression ◽  
Carbon Starvation ◽  
Nitrogen Catabolite Repression

scholarly journals Metabolic Engineering of the Regulators in Nitrogen Catabolite Repression To Reduce the Production of Ethyl Carbamate in a Model Rice Wine System

2013 ◽  
Vol 80 (1) ◽  
pp. 392-398 ◽  
Author(s):  
Xinrui Zhao ◽  
Huijun Zou ◽  
Jianwei Fu ◽  
Jingwen Zhou ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Nuclear Localization ◽  
Catabolite Repression ◽  
Genetically Modified ◽  
Nitrogen Sources ◽  
Ethyl Carbamate ◽  
Rice Wine ◽  
Nitrogen Catabolite Repression ◽  
Wine Production ◽  
Content Type

ABSTRACTRice wine has been one of the most popular traditional alcoholic drinks in China. However, the presence of potentially carcinogenic ethyl carbamate (EC) in rice wine has raised a series of food safety issues. During rice wine production, the key reason for EC formation is urea accumulation, which occurs because of nitrogen catabolite repression (NCR) inSaccharomyces cerevisiae. NCR represses urea utilization by retaining Gln3p in the cytoplasm when preferred nitrogen sources are present. In order to increase the nuclear localization of Gln3p, some possible phosphorylation sites on the nuclear localization signal were mutated and the nuclear localization regulation signal was truncated, and the disruption ofURE2provided an additional method of reducing urea accumulation. By combining these strategies, the genes involved in urea utilization (DUR1,2andDUR3) could be significantly activated in the presence of glutamine. During shake flask fermentations of the genetically modified strains, very little urea accumulated in the medium. Furthermore, the concentrations of urea and EC were reduced by 63% and 72%, respectively, in a model rice wine system. Examination of the normal nutrients in rice wine indicated that there were few differences in fermentation characteristics between the wild-type strain and the genetically modified strain. These results show that metabolic engineering of the NCR regulators has great potential as a method for eliminating EC during rice wine production.

scholarly journals Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Marcos D. Palavecino ◽  
Susana R. Correa-García ◽  
Mariana Bermúdez-Moretti
Keyword(s):  
Catabolite Repression ◽  
Transcriptional Control ◽  
Nitrogen Limitation ◽  
Temporal Order ◽  
Nitrogen Sources ◽  
Nitrogen Utilization ◽  
Aminobutyric Acid ◽  
General Mechanism ◽  
Nitrogen Catabolite Repression ◽  
Catabolic Enzymes

Yeast can use a wide variety of nitrogen compounds. However, the ability to synthesize enzymes and permeases for catabolism of poor nitrogen sources is limited in the presence of a rich one. This general mechanism of transcriptional control is called nitrogen catabolite repression. Poor nitrogen sources, such as leucine, γ-aminobutyric acid (GABA), and allantoin, enable growth after the synthesis of pathway-specific catabolic enzymes and permeases. This synthesis occurs only under conditions of nitrogen limitation and in the presence of a pathway-specific signal. In this work we studied the temporal order in the induction of AGP1, BAP2, UGA4, and DAL7, genes that are involved in the catabolism and use of leucine, GABA, and allantoin, three poor nitrogen sources. We found that when these amino acids are available, cells will express AGP1 and BAP2 in the first place, then DAL7, and at last UGA4. Dal81, a general positive regulator of genes involved in nitrogen utilization related to the metabolisms of GABA, leucine, and allantoin, plays a central role in this coordinated regulation.

scholarly journals Nuclear Gln3 Import Is Regulated by Nitrogen Catabolite Repression Whereas Export Is Specifically Regulated by Glutamine

Genetics ◽  
2015 ◽  
Vol 201 (3) ◽  
pp. 989-1016 ◽  
Author(s):  
Rajendra Rai ◽  
Jennifer J. Tate ◽  
Karthik Shanmuganatham ◽  
Martha M. Howe ◽  
David Nelson ◽  
...  
Keyword(s):  
Catabolite Repression ◽  
Nitrogen Catabolite Repression

scholarly journals Global Expression Analysis of the Yeast Lachancea (Saccharomyces) kluyveri Reveals NewURCGenes Involved in Pyrimidine Catabolism

2013 ◽  
Vol 13 (1) ◽  
pp. 31-42 ◽  
Author(s):  
Anna Andersson Rasmussen ◽  
Dineshkumar Kandasamy ◽  
Halfdan Beck ◽  
Seth D. Crosby ◽  
Olof Björnberg ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Catabolite Repression ◽  
Nitrogen Sources ◽  
Global Changes ◽  
Nitrogen Catabolite Repression ◽  
Content Type ◽  
Double Deletion ◽  
Pyrimidine Catabolism ◽  
Saccharomyces Kluyveri ◽  
Similar Expression Pattern

ABSTRACTPyrimidines are important nucleic acid precursors which are constantly synthesized, degraded, and rebuilt in the cell. Four degradation pathways, two of which are found in eukaryotes, have been described. One of them, theURCpathway, has been initially discovered in our laboratory in the yeastLachancea kluyveri. Here, we present the global changes in gene expression inL. kluyveriin response to different nitrogen sources, including uracil, uridine, dihydrouracil, and ammonia. The expression pattern of the knownURCgenes,URC1-6, helped to identify nine putative novelURCgenes with a similar expression pattern. The microarray analysis provided evidence that both theURCandPYDgenes are under nitrogen catabolite repression inL. kluyveriand are induced by uracil or dihydrouracil, respectively. We determined the function ofURC8, which was found to catalyze the reduction of malonate semialdehyde to 3-hydroxypropionate, the final degradation product of the pathway. The other eight genes studied were all putative permeases. Our analysis of double deletion strains showed that theL. kluyveriFui1p protein transported uridine, just like its homolog inSaccharomyces cerevisiae, but we demonstrated that is was not the only uridine transporter inL. kluyveri. We also showed that theL. kluyverihomologs ofDUR3andFUR4do not have the same function that they have inS. cerevisiae, where they transport urea and uracil, respectively. InL. kluyveri, both of these deletion strains grew normally on uracil and urea.

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