scholarly journals Once Phosphorylated, Tyrosines in Carboxyl Terminus of Protein-tyrosine Kinase Syk Interact with Signaling Proteins, Including TULA-2, a Negative Regulator of Mast Cell Degranulation

2012 ◽  
Vol 287 (11) ◽  
pp. 8194-8204 ◽  
Author(s):  
Rodrigo Orlandini de Castro ◽  
Juan Zhang ◽  
Jacqueline R. Groves ◽  
Emilia Alina Barbu ◽  
Reuben P. Siraganian
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Mast Cell Degranulation ◽  
Negative Regulator ◽  
Carboxyl Terminus ◽  
Signaling Proteins ◽  
Protein Tyrosine

scholarly journals Mast cell signaling: The role of protein tyrosine kinase Syk, its activation and screening methods for new pathway participants

FEBS Letters ◽  
2010 ◽  
Vol 584 (24) ◽  
pp. 4933-4940 ◽  
Author(s):  
Reuben P. Siraganian ◽  
Rodrigo O. de Castro ◽  
Emilia A. Barbu ◽  
Juan Zhang
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Cell Signaling ◽  
Protein Tyrosine Kinase ◽  
Screening Methods ◽  
Protein Tyrosine

The protein tyrosine kinase Tec regulates mast cell function

2009 ◽  
Vol 39 (11) ◽  
pp. 3228-3238 ◽  
Author(s):  
Uwe Schmidt ◽  
Anastasia Abramova ◽  
Nicole Boucheron ◽  
Eva Eckelhart ◽  
Alexandra Schebesta ◽  
...  
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Cell Function ◽  
Protein Tyrosine

scholarly journals SH2 Domain-Mediated Interaction of Inhibitory Protein Tyrosine Kinase Csk with Protein Tyrosine Phosphatase-HSCF

2001 ◽  
Vol 21 (4) ◽  
pp. 1077-1088 ◽  
Author(s):  
Bing Wang ◽  
Serge Lemay ◽  
Schickwann Tsai ◽  
André Veillette
Keyword(s):  
Signal Transduction ◽  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Protein Tyrosine Phosphatases ◽  
Kinase Domain ◽  
Sh2 Domain ◽  
Negative Regulator ◽  
Yeast Cells ◽  
Inhibitory Protein ◽  
Protein Tyrosine

ABSTRACT The protein tyrosine kinase (PTK) Csk is a potent negative regulator of several signal transduction processes, as a consequence of its exquisite ability to inactivate Src-related PTKs. This function requires not only the kinase domain of Csk, but also its Src homology 3 (SH3) and SH2 regions. We showed previously that the Csk SH3 domain mediates highly specific associations with two members of the PEP family of nonreceptor protein tyrosine phosphatases (PTPs), PEP and PTP-PEST. In comparison, the Csk SH2 domain interacts with several tyrosine phosphorylated molecules, presumed to allow targetting of Csk to sites of Src family kinase activation. Herein, we attempted to understand better the regulation of Csk by identifying ligands for its SH2 domain. Using a modified yeast two-hybrid screen, we uncovered the fact that Csk associates with PTP-HSCF, the third member of the PEP family of PTPs. This association was documented not only in yeast cells but also in a heterologous mammalian cell system and in cytokine-dependent hemopoietic cells. Surprisingly, the Csk–PTP-HSCF interaction was found to be mediated by the Csk SH2 domain and two putative sites of tyrosine phosphorylation in the noncatalytic portion of PTP-HSCF. Transfection experiments indicated that Csk and PTP-HSCF synergized to inhibit signal transduction by Src family kinases and that this cooperativity was dependent on the domains mediating their association. Finally, we obtained evidence that PTP-HSCF inactivated Src-related PTKs by selectively dephosphorylating the positive regulatory tyrosine in their kinase domain. Taken together, these results demonstrate that part of the function of the Csk SH2 domain is to mediate an inducible association with a PTP, thereby engineering a more efficient inhibitory mechanism for Src-related PTKs. Coupled with previously published observations, these data also establish that Csk forms complexes with all three known members of the PEP family.

scholarly journals Negative regulation of Lyn protein-tyrosine kinase by c-Cbl ubiquitin-protein ligase in FcɛRI-mediated mast cell activation

2003 ◽  
Vol 8 (10) ◽  
pp. 825-836 ◽  
Author(s):  
Shinkou Kyo ◽  
Kiyonao Sada ◽  
Xiujuan Qu ◽  
Koichiro Maeno ◽  
S. M. Shahjahan Miah ◽  
...  
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Cell Activation ◽  
Negative Regulation ◽  
Mast Cell Activation ◽  
Protein Tyrosine ◽  
Ubiquitin Protein Ligase

Protein tyrosine kinase Syk in mast cell signaling

2002 ◽  
Vol 38 (16-18) ◽  
pp. 1229-1233 ◽  
Author(s):  
Reuben P Siraganian ◽  
Juan Zhang ◽  
Katsuhiro Suzuki ◽  
Kiyonao Sada
Keyword(s):  
Mast Cell ◽  
Tyrosine Kinase ◽  
Cell Signaling ◽  
Protein Tyrosine Kinase ◽  
Protein Tyrosine
Sign in / Sign up

Export Citation Format

Share Document