scholarly journals Characterization of a Cluster of Human High/Ultrahigh Sulfur Keratin-associated Protein Genes Embedded in the Type I Keratin Gene Domain on Chromosome 17q12-21

2001 ◽  
Vol 276 (22) ◽  
pp. 19440-19451 ◽  
Author(s):  
Michael A. Rogers ◽  
Lutz Langbein ◽  
Hermelita Winter ◽  
Claudia Ehmann ◽  
Silke Praetzel ◽  
...  
Keyword(s):  
Type I ◽  
Keratin Gene

scholarly journals Genomic Characterization of the Human Type I Cuticular Hair Keratin hHa2 and Identification of an Adjacent Novel Type I Hair Keratin Gene hHa5

1996 ◽  
Vol 107 (4) ◽  
pp. 633-638 ◽  
Author(s):  
Michael A Rogers ◽  
Hermelita Winter ◽  
Lutz Langbein ◽  
Thomas Krieg ◽  
Jürgen Schweizer
Keyword(s):  
Type I ◽  
Keratin Gene ◽  
Human Type ◽  
Hair Keratin ◽  
Genomic Characterization

The human type I keratin gene family: Characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain

2004 ◽  
Vol 72 (9-10) ◽  
pp. 527-540 ◽  
Author(s):  
Michael A. Rogers ◽  
Hermelita Winter ◽  
Lutz Langbein ◽  
Raphael Bleiler ◽  
Jürgen Schweizer
Keyword(s):  
Gene Family ◽  
Hair Follicle ◽  
Type I ◽  
Keratin Gene ◽  
Human Type

Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis

2010 ◽  
Vol 239 (12) ◽  
pp. 3172-3181 ◽  
Author(s):  
Ken-ichi T. Suzuki ◽  
Keiko Kashiwagi ◽  
Motomu Ujihara ◽  
Takuzo Marukane ◽  
Akira Tazaki ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Reporter Genes ◽  
Type I ◽  
Fluorescent Reporter ◽  
Keratin Gene ◽  
Transgenic Lines ◽  
Fluorescent Reporter Genes

Characterization of type I-F CRISPR-Cas system in Laribacter hongkongensis isolates from animals, the environment and diarrhea patients

2021 ◽  
Vol 346 ◽  
pp. 109153
Author(s):  
Ling Wang ◽  
Li Wang ◽  
Youzhao Liu ◽  
Zhiyun Wang ◽  
Qing Chen ◽  
...  
Keyword(s):  
Type I ◽  
Laribacter Hongkongensis

scholarly journals Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B

2021 ◽  
Vol 22 (5) ◽  
pp. 2501
Author(s):  
Sonja Hinz ◽  
Dominik Jung ◽  
Dorota Hauert ◽  
Hagen S. Bachmann
Keyword(s):  
Protein Function ◽  
Tumor Development ◽  
Cysteine Residue ◽  
Type I ◽  
Pharmacological Characterization ◽  
Anti Cancer ◽  
And Function ◽  
Caax Motif ◽  
Important Drug

Geranylgeranyltransferase type-I (GGTase-I) represents an important drug target since it contributes to the function of many proteins that are involved in tumor development and metastasis. This led to the development of GGTase-I inhibitors as anti-cancer drugs blocking the protein function and membrane association of e.g., Rap subfamilies that are involved in cell differentiation and cell growth. In the present study, we developed a new NanoBiT assay to monitor the interaction of human GGTase-I and its substrate Rap1B. Different Rap1B prenylation-deficient mutants (C181G, C181S, and ΔCQLL) were designed and investigated for their interaction with GGTase-I. While the Rap1B mutants C181G and C181S still exhibited interaction with human GGTase-I, mutant ΔCQLL, lacking the entire CAAX motif (defined by a cysteine residue, two aliphatic residues, and the C-terminal residue), showed reduced interaction. Moreover, a specific, peptidomimetic and competitive CAAX inhibitor was able to block the interaction of Rap1B with GGTase-I. Furthermore, activation of both Gαs-coupled human adenosine receptors, A2A (A2AAR) and A2B (A2BAR), increased the interaction between GGTase-I and Rap1B, probably representing a way to modulate prenylation and function of Rap1B. Thus, A2AAR and A2BAR antagonists might be promising candidates for therapeutic intervention for different types of cancer that overexpress Rap1B. Finally, the NanoBiT assay provides a tool to investigate the pharmacology of GGTase-I inhibitors.

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