Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis

2010 ◽  
Vol 239 (12) ◽  
pp. 3172-3181 ◽  
Author(s):  
Ken-ichi T. Suzuki ◽  
Keiko Kashiwagi ◽  
Motomu Ujihara ◽  
Takuzo Marukane ◽  
Akira Tazaki ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Reporter Genes ◽  
Type I ◽  
Fluorescent Reporter ◽  
Keratin Gene ◽  
Transgenic Lines ◽  
Fluorescent Reporter Genes

scholarly journals Genomic Characterization of the Human Type I Cuticular Hair Keratin hHa2 and Identification of an Adjacent Novel Type I Hair Keratin Gene hHa5

1996 ◽  
Vol 107 (4) ◽  
pp. 633-638 ◽  
Author(s):  
Michael A Rogers ◽  
Hermelita Winter ◽  
Lutz Langbein ◽  
Thomas Krieg ◽  
Jürgen Schweizer
Keyword(s):  
Type I ◽  
Keratin Gene ◽  
Human Type ◽  
Hair Keratin ◽  
Genomic Characterization

Use of fluorescent reporter genes in olive (Olea europaea L.) transformation

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Sergio Cerezo ◽  
Elena Palomo-Ríos ◽  
Sinda Ben Mariem ◽  
José A. Mercado ◽  
Fernando Pliego-Alfaro
Keyword(s):  
Olea Europaea ◽  
Reporter Genes ◽  
Fluorescent Reporter ◽  
Olea Europaea L ◽  
Fluorescent Reporter Genes ◽  
Olea Europaéa

scholarly journals Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection

2018 ◽  
Author(s):  
Valentin M. Sluch ◽  
Xitiz Chamling ◽  
Claire Wenger ◽  
Yukan Duan ◽  
Dennis S. Rice ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Low Frequency ◽  
Reporter Genes ◽  
Specific Cell ◽  
Fluorescent Reporter ◽  
Antibiotic Selection ◽  
Homology Directed Repair ◽  
Human And Mouse ◽  
Fluorescent Reporter Genes

AbstractPluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However, human PSCs are known to have a low frequency of gene knock-in (KI) by HDR, making reporter line generation an arduous process. Here, we report a methodology for scarless KI of large fluorescent reporter genes into PSCs by transient selection with puromycin or zeocin. With this method, we can perform targeted KI of a single reporter gene with up to 65% efficiency, as well as simultaneous KI of two reporter genes into different loci with up to 11% efficiency. Additionally, we demonstrate that this method also works in mouse PSCs.

scholarly journals Characterization of a Cluster of Human High/Ultrahigh Sulfur Keratin-associated Protein Genes Embedded in the Type I Keratin Gene Domain on Chromosome 17q12-21

2001 ◽  
Vol 276 (22) ◽  
pp. 19440-19451 ◽  
Author(s):  
Michael A. Rogers ◽  
Lutz Langbein ◽  
Hermelita Winter ◽  
Claudia Ehmann ◽  
Silke Praetzel ◽  
...  
Keyword(s):  
Type I ◽  
Keratin Gene

The human type I keratin gene family: Characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain

2004 ◽  
Vol 72 (9-10) ◽  
pp. 527-540 ◽  
Author(s):  
Michael A. Rogers ◽  
Hermelita Winter ◽  
Lutz Langbein ◽  
Raphael Bleiler ◽  
Jürgen Schweizer
Keyword(s):  
Gene Family ◽  
Hair Follicle ◽  
Type I ◽  
Keratin Gene ◽  
Human Type

scholarly journals Recombination Can Lead to Spurious Results in Retroviral Transduction with Dually Fluorescent Reporter Genes

2013 ◽  
Vol 87 (24) ◽  
pp. 13900-13903 ◽  
Author(s):  
D. J. Salamango ◽  
D. A. Evans ◽  
M. F. Baluyot ◽  
J. N. Furlong ◽  
M. C. Johnson
Keyword(s):  
Reporter Genes ◽  
Fluorescent Reporter ◽  
Retroviral Transduction ◽  
Spurious Results ◽  
Fluorescent Reporter Genes
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