scholarly journals Endothelial Differentiation Gene-2 Receptor Is Involved in Lysophosphatidic Acid-dependent Control of 3T3F442A Preadipocyte Proliferation and Spreading

2001 ◽  
Vol 276 (15) ◽  
pp. 11599-11605 ◽  
Author(s):  
Céline Pagès ◽  
Danièle Daviaud ◽  
Songzhu An ◽  
Stéphane Krief ◽  
Max Lafontan ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Significant Contribution ◽  
Cell Spreading ◽  
Endothelial Differentiation ◽  
Receptor Subtype ◽  
Strong Reduction ◽  
Chain Reaction ◽  
Biological Responses ◽  
Polymerase Chain ◽  
Differentiation Gene

EDG-2,EDG-4,EDG-7, andPSP24genes encode distinct lysophosphatidic acid (LPA) receptors. The aim of the present study was to determine which receptor subtype is involved in the biological responses generated by LPA in preadipocytes. Growing 3T3F442A preadipocytes expressEDG-2andEDG-4mRNAs, with no expression ofEDG-7orPSP24mRNAs. Quantitative reverse transcriptase-polymerase chain reaction revealed thatEDG-2transcripts were 10-fold more abundant than that ofEDG-4. To determine the involvement of theEDG-2receptor in the responses of growing preadipocytes to LPA, stable transfection of antisenseEDG-2cDNA was performed in growing 3T3F442A preadipocytes. This procedure, led to a significant and specific reduction inEDG-2mRNA and protein. This was associated with a significant alteration in the effect of LPA on both cell proliferation and cell spreading. Finally, the differentiation of growing preadipocytes into quiescent adipocytes led to a strong reduction in the level ofEDG-2transcripts. Results demonstrate the significant contribution of the EDG-2 receptor in the biological responses generated by LPA in 3T3F442A preadipocytes.

scholarly journals Effect of a Precision Cryotherapy Device With Temperature- Adjustability on Mice With Lysophosphatidic Acid-Induced Pruritus

2021 ◽  
Author(s):  
Mi Hee Kwack ◽  
Seongjin Lee ◽  
Han Jin jung ◽  
Gi Ung Ha ◽  
Gun-Ho Kim ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Adverse Impact ◽  
Complex Mechanism ◽  
Inflammatory Skin Disease ◽  
Chain Reaction ◽  
Future Studies ◽  
Chronic Itch ◽  
Before And After ◽  
Polymerase Chain

Abstract Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease that is known to cause a significant adverse impact on the quality of life of patients. Reducing chronic itch that has a complex mechanism is one of the most important challenges in AD treatment.Objective: To evaluate the effect of a temperature-adjustable cryotherapy device on mice with lysophosphatidic acid-induced pruritus.Methods: A temperature and time-adjustable cryotherapy device was used for the treatment of lysophosphatidic acid-induced pruritus of mice in the following conditions: −5℃, 0℃, or 5℃ for 5 sec, 10 sec, or 20 sec. Expression of itch-related biomarkers before and after modulation of temperature was investigated with real-time polymerase chain reaction (PCR) and immunohistochemistry.Results: Expression of itch-related biomarkers was decreased after modulation of temperature. For gene expression, all were decreased at 5℃ for 10 sec and 20 sec, and at 0℃ for 5 sec, 10 sec, and 20 sec. For protein expression, all were decreased at 5℃ for 10 sec and 20 sec, and at 0℃ for 5 sec and 10 sec.Conclusion: This study demonstrates the pruritus-relieving effect of the temperature-adjustable device on mice. This may provide some evidence for future studies on patients with mild AD.

scholarly journals Hepatic Oval (Stem) Cell Expression of Endothelial Differentiation Gene Receptors for Lysophosphatidic Acid in Mouse Chronic Liver Injury

2002 ◽  
Vol 11 (4) ◽  
pp. 643-649 ◽  
Author(s):  
Yuri Y. Sautin ◽  
Marda Jorgensen ◽  
Bryon E. Petersen ◽  
Jean S. Saulnier-Blache ◽  
James M. Crawford ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Injury ◽  
Lysophosphatidic Acid ◽  
Chronic Liver ◽  
Endothelial Differentiation ◽  
Chronic Liver Injury ◽  
Cell Expression ◽  
Differentiation Gene

scholarly journals Subtype-specific Residues Involved in Ligand Activation of the Endothelial Differentiation Gene Family Lysophosphatidic Acid Receptors

2008 ◽  
Vol 283 (18) ◽  
pp. 12175-12187 ◽  
Author(s):  
William J. Valentine ◽  
James I. Fells ◽  
Donna H. Perygin ◽  
Sana Mujahid ◽  
Kazuaki Yokoyama ◽  
...  
Keyword(s):  
Gene Family ◽  
Lysophosphatidic Acid ◽  
Endothelial Differentiation ◽  
Ligand Activation ◽  
Differentiation Gene ◽  
Lysophosphatidic Acid Receptors

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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