scholarly journals Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

2008 ◽  
Vol 105 (47) ◽  
pp. 18602-18607 ◽  
Author(s):  
Y.-S. Lee ◽  
S.-L. Choi ◽  
T.-H. Kim ◽  
J.-A Lee ◽  
H. K. Kim ◽  
...  
Keyword(s):  
Transcriptome Analysis

scholarly journals Transcriptome Analysis of Wounding in the Model Grass Lolium temulentum

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 780
Author(s):  
James E. Dombrowski ◽  
Brent A. Kronmiller ◽  
Vicky Hollenbeck ◽  
Ruth C. Martin
Keyword(s):  
Transcriptome Analysis ◽  
Proteinase Inhibitors ◽  
Wrky Transcription Factors ◽  
Lolium Temulentum ◽  
Mitogen Activated Protein ◽  
Hormone Biosynthesis ◽  
Model Grass ◽  
Molecular Components ◽  
Rapid Regrowth

For forage and turf grasses, wounding is a predominant stress that often results in extensive loss of vegetative tissues followed by rapid regrowth. Currently, little is known concerning the perception, signaling, or molecular responses associated with wound stress in forage- and turf-related grasses. A transcriptome analysis of Lolium temulentum plants subjected to severe wounding revealed 9413 upregulated and 7704 downregulated, distinct, differentially expressed genes (DEGs). Categories related to signaling, transcription, and response to stimuli were enriched in the upregulated DEGs. Specifically, sequences annotated as enzymes involved in hormone biosynthesis/action and cell wall modifications, mitogen-activated protein kinases, WRKY transcription factors, proteinase inhibitors, and pathogen defense-related DEGs were identified. Surprisingly, DEGs related to heat shock and chaperones were more prevalent in the downregulated DEGs when compared with the upregulated DEGs. This wound transcriptome analysis is the first step in identifying the molecular components and pathways used by grasses in response to wounding. The information gained from the analysis will provide a valuable molecular resource that will be used to develop approaches that can improve the recovery, regrowth, and long-term fitness of forage and turf grasses before/after cutting or grazing.

scholarly journals A comprehensive transcriptome analysis reveals broader but weaker host response of SARS-CoV-2 than SARS-CoV

2020 ◽  
Author(s):  
Chi Sum Leung ◽  
Songhong Xie ◽  
Jiali Feng ◽  
Dongjing Chen ◽  
Aimei Dai
Keyword(s):  
Immune Responses ◽  
Transcriptome Analysis ◽  
Tissue Specificity ◽  
Immune Therapy ◽  
Host Responses ◽  
Human Populations ◽  
Lung Cancer Cell ◽  
Health Crisis ◽  
Epidemiology Studies

COVID-19, which has resulted a worldwide health crisis with more than 74.9 million confirmed cases worldwide by December 2020, is caused by a newly emerging coronavirus identified and named SARS-CoV-2 in February in Wuhan, China. Experiences in defeating SARS, which infested during 2002-2003, can be used in treating the new disease. However, comparative genomics and epidemiology studies have shown much difference between SARS-CoV and SARS-CoV-2, which underlies the different clinical features and therapies in between those two diseases. Further studies comparing transcriptomes infected by these two viruses to uncover the differences in host responses would be necessary. Here we conducted a comprehensive transcriptome analysis of SARS-CoV and SARS-CoV-2-infected human cell lines, including Caco-2, Calu-3, H1299. Clustering analysis and expression of ACE2 show that SARS-CoV-2 has broader but weaker infection, where the largest discrepancy occurs in the epithelial lung cancer cell, Calu-3. SARS-CoV-2 genes also show less tissue specificity than SARS-CoV genes. Furthermore, we detected more general but moderate immune responses in SARS-CoV-2 infected transcriptomes by comparing weighted gene co-expression networks and modules. Our results suggest a different immune therapy and treatment scheme for COVID-19 patients than the ones used on SARS patients. The wider but weaker permissiveness and host responses of virus infection may also imply a long-term existence of SARS-CoV-2 among human populations.

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