scholarly journals Transcriptome analysis of Drosophila melanogaster laboratory strains of different geographical origin after long‐term laboratory maintenance

2020 ◽  
Vol 10 (14) ◽  
pp. 7082-7093
Author(s):  
Mikhail Zarubin ◽  
Alena Yakhnenko ◽  
Elena Kravchenko
Keyword(s):  
Drosophila Melanogaster ◽  
Transcriptome Analysis ◽  
Geographical Origin ◽  
Laboratory Maintenance

scholarly journals The Rate of Mutation and the Homozygous and Heterozygous Mutational Effects for Competitive Viability: A Long-Term Experiment With Drosophila melanogaster

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 681-693 ◽  
Author(s):  
David Chavarrías ◽  
Carlos López-Fanjul ◽  
Aurora García-Dorado
Keyword(s):  
Drosophila Melanogaster ◽  
Minimum Distance ◽  
Deleterious Mutations ◽  
Regression Estimate ◽  
Term Experiment ◽  
Degree Of Dominance ◽  
Long Term Experiment ◽  
Rate Of Decline ◽  
Good Agreement

Abstract The effect of 250 generations of mutation accumulation (MA) on the second chromosome competitive viability of Drosophila melanogaster was analyzed both in homozygous and heterozygous conditions. We used full-sib MA lines, where selection hampers the accumulation of severely deleterious mutations but is ineffective against mildly deleterious ones. A large control population was simultaneously evaluated. Competitive viability scores, unaffected by the expression of mutations in heterozygosis, were obtained relative to a Cy/L2 genotype. The rate of decline in mean ΔM ≈ 0.1% was small. However, that of increase in variance ΔV ≈ 0.08 × 10-3 was similar to the values obtained in previous experiments when severely deleterious mutations were excluded. The corresponding estimates of the mutation rate λ ≥ 0.01 and the average effect of mutations E(s) ≤ 0.08 are in good agreement with Bateman-Mukai and minimum distance estimates for noncompetitive viability obtained from the same MA lines after 105 generations. Thus, competitive and noncompetitive viability show similar mutational properties. The regression estimate of the degree of dominance for mild-to-moderate deleterious mutations was ∼0.3, suggesting that the pertinent value for new unselected mutations should be somewhat smaller.

Preservation of diversity and oenological properties of wine yeasts during long-term laboratory maintenance: A study of strains of a century-old Tokaj wine yeast collection

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 789-798 ◽  
Author(s):  
Zoltán Kállai ◽  
Walter P. Pfliegler ◽  
Judit Mitercsák ◽  
Gergő Szendei ◽  
Matthias Sipiczki
Keyword(s):  
Wine Yeast ◽  
Wine Yeasts ◽  
Laboratory Maintenance

Testing the “rate of living” model: further evidence that longevity and metabolic rate are not inversely correlated in Drosophila melanogaster

2004 ◽  
Vol 97 (5) ◽  
pp. 1915-1922 ◽  
Author(s):  
Wayne A. Van Voorhies ◽  
Aziz A. Khazaeli ◽  
James W. Curtsinger
Keyword(s):  
Drosophila Melanogaster ◽  
Metabolic Rate ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Time Of Day ◽  
Metabolic Rates ◽  
Extended Longevity ◽  
Flow Through ◽  
The Relationship

In a recent study examining the relationship between longevity and metabolism in a large number of recombinant inbred Drosophila melanogaster lines, we found no indication of the inverse relationship between longevity and metabolic rate that one would expect under the classical “rate of living” model. A potential limitation in generalizing from that study is that it was conducted on experimental material derived from a single set of parental strains originally developed over 20 years ago. To determine whether the observations made with those lines are characteristic of the species, we studied metabolic rates and longevities in a second, independently derived set of recombinant inbred lines. We found no correlation in these lines between metabolic rate and longevity, indicating that the ability to both maintain a normal metabolic rate and have extended longevity may apply to D. melanogaster in general. To determine how closely our measurements reflect metabolic rates of flies maintained under conditions of life span assays, we used long-term, flow-through metabolic rate measurements and closed system respirometry to examine the effects of variables such as time of day, feeding state, fly density, mobility of the flies, and nitrogen knockout on D. melanogaster metabolic rate. We found that CO2 production estimated in individual flies accurately reflects metabolic rates of flies under the conditions used for longevity assays.

scholarly journals Metabolic effects of CO 2 anaesthesia in Drosophila melanogaster

2012 ◽  
Vol 8 (6) ◽  
pp. 1050-1054 ◽  
Author(s):  
H. Colinet ◽  
D. Renault
Keyword(s):  
Drosophila Melanogaster ◽  
Recovery Period ◽  
Metabolic Changes ◽  
Metabolic Effects ◽  
Short Term ◽  
Short And Long Term ◽  
Metabolic Variation ◽  
Negative Side Effects ◽  
Entomological Research

Immobilization of insects is necessary for various experimental purposes, and CO 2 exposure remains the most popular anaesthetic method in entomological research. A number of negative side effects of CO 2 anaesthesia have been reported, but CO 2 probably brings about metabolic modifications that are poorly known. In this work, we used GC/MS-based metabolic fingerprinting to assess the effect of CO 2 anaesthesia in Drosophila melanogaster adults. We analysed metabolic variation of flies submitted to acute CO 2 exposure and assessed the temporal metabolic changes during short- and long-term recovery. We found that D. melanogaster metabotypes were significantly affected by the anaesthetic treatment. Metabolic changes caused by acute CO 2 exposure were still manifested after 14 h of recovery. However, we found no evidence of metabolic alterations when a long recovery period was allowed (more than 24 h). This study points to some metabolic pathways altered during CO 2 anaesthesia (e.g. energetic metabolism). Evidence of short-term metabolic changes indicates that CO 2 anaesthesia should be used with utmost caution in physiological studies when a short recovery is allowed. In spite of this, CO 2 treatment seems to be an acceptable anaesthetic method provided that a long recovery period is allowed (more than 24 h).

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