scholarly journals Lack of aldose 1-epimerase in Hypocrea jecorina (anamorph Trichoderma reesei): A key to cellulase gene expression on lactose

2008 ◽  
Vol 105 (20) ◽  
pp. 7141-7146 ◽  
Author(s):  
E. Fekete ◽  
B. Seiboth ◽  
C. P. Kubicek ◽  
A. Szentirmai ◽  
L. Karaffa
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Hypocrea Jecorina

scholarly journals Dehydrogenase GRD1 Represents a Novel Component of the Cellulase Regulon in Trichoderma reesei (Hypocrea jecorina)

2011 ◽  
Vol 77 (13) ◽  
pp. 4553-4563 ◽  
Author(s):  
André Schuster ◽  
Christian P. Kubicek ◽  
Monika Schmoll
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Carbon Sources ◽  
Cellulosic Biomass ◽  
Biofuel Production ◽  
Dependent Manner ◽  
Cellulase Gene ◽  
Hypocrea Jecorina ◽  
Content Type ◽  
Transcription Expression

ABSTRACTTrichoderma reesei(Hypocrea jecorina) is nowadays the most important industrial producer of cellulase and hemicellulase enzymes, which are used for pretreatment of cellulosic biomass for biofuel production. In this study, we introduce a novel component, GRD1 (glucose-ribitol dehydrogenase 1), which shows enzymatic activity on cellobiose and positively influences cellulase gene transcription, expression, and extracellular endo-1,4-β-d-glucanase activity.grd1is differentially transcribed upon growth on cellulose and the induction of cellulase gene expression by sophorose. The transcription ofgrd1is coregulated with that ofcel7a(cbh1) under inducing conditions. GRD1 is further involved in carbon source utilization on several carbon sources, such as those involved in lactose andd-galactose catabolism, in several cases in a light-dependent manner. We conclude that GRD1 represents a novel enhancer of cellulase gene expression, which by coregulation with the major cellulase may act via optimization of inducing mechanisms.

Mitochondrial functions mediate cellulase gene expression in Trichoderma reesei

Biochemistry ◽  
1995 ◽  
Vol 34 (33) ◽  
pp. 10456-10462 ◽  
Author(s):  
J. Abrahao Neto ◽  
C. H. B. Rossini ◽  
S. El-Gogary ◽  
F. Henrique-Silva ◽  
O. Crivellaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Mitochondrial Functions

A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

2019 ◽  
Vol 103 (11) ◽  
pp. 4511-4523 ◽  
Author(s):  
Lei Wang ◽  
Xinxing Lv ◽  
Yanli Cao ◽  
Fanglin Zheng ◽  
Xiangfeng Meng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Transcriptional Regulator ◽  
Cellulase Gene

scholarly journals The VELVET A Orthologue VEL1 of Trichoderma reesei Regulates Fungal Development and Is Essential for Cellulase Gene Expression

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112799 ◽  
Author(s):  
Razieh Karimi Aghcheh ◽  
Zoltán Németh ◽  
Lea Atanasova ◽  
Erzsébet Fekete ◽  
Melinda Paholcsek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Fungal Development

scholarly journals Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II

PLoS Genetics ◽  
2020 ◽  
Vol 16 (9) ◽  
pp. e1008979
Author(s):  
Fanglin Zheng ◽  
Yanli Cao ◽  
Renfei Yang ◽  
Lei Wang ◽  
Xinxing Lv ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Trichoderma Reesei ◽  
Cellulase Gene

Gluconolactone induces cellulase gene expression in cellulolytic filamentous fungus Trichoderma reesei

RSC Advances ◽  
2014 ◽  
Vol 4 (68) ◽  
pp. 36057-36063
Author(s):  
Yanbo Kou ◽  
Jintao Xu ◽  
Yanli Cao ◽  
Xinxing Lv ◽  
Guolei Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Cellulase Gene

Gluconolactone, an inhibitor of β-glucosidase, is capable of inducing cellulase gene expression in cellulolytic filamentous fungusTrichoderma reesei.

scholarly journals ACE4, a novel transcriptional activator involved in the regulation of cellulase genes on cellulose in Trichoderma reesei

2021 ◽  
Author(s):  
Yumeng Chen ◽  
Aibo Lin ◽  
Pei Liu ◽  
Xingjia Fan ◽  
Chuan Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Trichoderma Reesei ◽  
Transcriptional Activator ◽  
Cellulase Production ◽  
Comparative Genomic ◽  
Transcriptional Level ◽  
Cellulase Gene ◽  
Cellulase Expression

The filamentous fungus Trichoderma reesei is a model strain for cellulase production. Cellulase gene expression in T. reesei is controlled by multiple transcription factors. Here, we identified by comparative genomic screening a novel transcriptional activator ACE4 ( A ctivator of c ellulase e xpression 4) that positively regulates cellulase gene expression on cellulose in T. reesei . Disruption of the ace4 gene significantly decreased expression of four main cellulase genes, and the essential cellulase transcription factor encoding gene ace3 . Overexpression of ace4 increased cellulase production by approximately 22% compared to that in the parental strain. Further investigations using electrophoretic mobility shift assays, DNase I footprinting assays, and chromatin immunoprecipitation assays indicated that ACE4 directly binds to the promoter of cellulase genes by recognizing the two adjacent 5′-GGCC-3′ sequences. Additionally, ACE4 directly binds to the promoter of ace3 and, in turn, regulates the expression of ACE3 to facilitate cellulase production. Collectively, these results demonstrate an important role for ACE4 in regulating cellulase gene expression, which will contribute to understanding the mechanism underlying cellulase expression in T. reesei . IMPORTANCE T. reesei is commonly utilized in industry to produce cellulases, enzymes that degrade lignocellulosic biomass for the production of bioethanol and bio-based products. T. reesei is capable of rapidly initiating the biosynthesis of cellulases in the presence of cellulose, which has made it useful as a model fungus for studying gene expression in eukaryotes. Cellulase gene expression is controlled through multiple transcription factors at the transcriptional level. However, the molecular mechanisms by which transcription is controlled remain unclear. In the present study, we identified a novel transcription factor, ACE4, which regulates cellulase expression on cellulose by binding to the promoters of cellulase genes and the cellulase activator ace3 . Our study not only expands the general functional understanding of the novel transcription factor ACE4 but also provides evidence for the regulatory mechanism mediating gene expression in T. reesei .

scholarly journals Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.

1997 ◽  
Vol 63 (4) ◽  
pp. 1298-1306 ◽  
Author(s):  
M Ilmén ◽  
A Saloheimo ◽  
M L Onnela ◽  
M E Penttilä
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Cellulase Gene
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