Mitochondrial functions mediate cellulase gene expression in Trichoderma reesei

Biochemistry ◽  
1995 ◽  
Vol 34 (33) ◽  
pp. 10456-10462 ◽  
Author(s):  
J. Abrahao Neto ◽  
C. H. B. Rossini ◽  
S. El-Gogary ◽  
F. Henrique-Silva ◽  
O. Crivellaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Mitochondrial Functions

A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

2019 ◽  
Vol 103 (11) ◽  
pp. 4511-4523 ◽  
Author(s):  
Lei Wang ◽  
Xinxing Lv ◽  
Yanli Cao ◽  
Fanglin Zheng ◽  
Xiangfeng Meng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Transcriptional Regulator ◽  
Cellulase Gene

scholarly journals The VELVET A Orthologue VEL1 of Trichoderma reesei Regulates Fungal Development and Is Essential for Cellulase Gene Expression

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112799 ◽  
Author(s):  
Razieh Karimi Aghcheh ◽  
Zoltán Németh ◽  
Lea Atanasova ◽  
Erzsébet Fekete ◽  
Melinda Paholcsek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Fungal Development

scholarly journals Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II

PLoS Genetics ◽  
2020 ◽  
Vol 16 (9) ◽  
pp. e1008979
Author(s):  
Fanglin Zheng ◽  
Yanli Cao ◽  
Renfei Yang ◽  
Lei Wang ◽  
Xinxing Lv ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Trichoderma Reesei ◽  
Cellulase Gene

Gluconolactone induces cellulase gene expression in cellulolytic filamentous fungus Trichoderma reesei

RSC Advances ◽  
2014 ◽  
Vol 4 (68) ◽  
pp. 36057-36063
Author(s):  
Yanbo Kou ◽  
Jintao Xu ◽  
Yanli Cao ◽  
Xinxing Lv ◽  
Guolei Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Cellulase Gene

Gluconolactone, an inhibitor of β-glucosidase, is capable of inducing cellulase gene expression in cellulolytic filamentous fungusTrichoderma reesei.

scholarly journals ACE4, a novel transcriptional activator involved in the regulation of cellulase genes on cellulose in Trichoderma reesei

2021 ◽  
Author(s):  
Yumeng Chen ◽  
Aibo Lin ◽  
Pei Liu ◽  
Xingjia Fan ◽  
Chuan Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Trichoderma Reesei ◽  
Transcriptional Activator ◽  
Cellulase Production ◽  
Comparative Genomic ◽  
Transcriptional Level ◽  
Cellulase Gene ◽  
Cellulase Expression

The filamentous fungus Trichoderma reesei is a model strain for cellulase production. Cellulase gene expression in T. reesei is controlled by multiple transcription factors. Here, we identified by comparative genomic screening a novel transcriptional activator ACE4 ( A ctivator of c ellulase e xpression 4) that positively regulates cellulase gene expression on cellulose in T. reesei . Disruption of the ace4 gene significantly decreased expression of four main cellulase genes, and the essential cellulase transcription factor encoding gene ace3 . Overexpression of ace4 increased cellulase production by approximately 22% compared to that in the parental strain. Further investigations using electrophoretic mobility shift assays, DNase I footprinting assays, and chromatin immunoprecipitation assays indicated that ACE4 directly binds to the promoter of cellulase genes by recognizing the two adjacent 5′-GGCC-3′ sequences. Additionally, ACE4 directly binds to the promoter of ace3 and, in turn, regulates the expression of ACE3 to facilitate cellulase production. Collectively, these results demonstrate an important role for ACE4 in regulating cellulase gene expression, which will contribute to understanding the mechanism underlying cellulase expression in T. reesei . IMPORTANCE T. reesei is commonly utilized in industry to produce cellulases, enzymes that degrade lignocellulosic biomass for the production of bioethanol and bio-based products. T. reesei is capable of rapidly initiating the biosynthesis of cellulases in the presence of cellulose, which has made it useful as a model fungus for studying gene expression in eukaryotes. Cellulase gene expression is controlled through multiple transcription factors at the transcriptional level. However, the molecular mechanisms by which transcription is controlled remain unclear. In the present study, we identified a novel transcription factor, ACE4, which regulates cellulase expression on cellulose by binding to the promoters of cellulase genes and the cellulase activator ace3 . Our study not only expands the general functional understanding of the novel transcription factor ACE4 but also provides evidence for the regulatory mechanism mediating gene expression in T. reesei .

scholarly journals Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.

1997 ◽  
Vol 63 (4) ◽  
pp. 1298-1306 ◽  
Author(s):  
M Ilmén ◽  
A Saloheimo ◽  
M L Onnela ◽  
M E Penttilä
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Cellulase Gene

scholarly journals Roles of Protein Kinase A and Adenylate Cyclase in Light-Modulated Cellulase Regulation in Trichoderma reesei

2012 ◽  
Vol 78 (7) ◽  
pp. 2168-2178 ◽  
Author(s):  
André Schuster ◽  
Doris Tisch ◽  
Verena Seidl-Seiboth ◽  
Christian P. Kubicek ◽  
Monika Schmoll
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Adenylate Cyclase ◽  
Protein Kinase A ◽  
Trichoderma Reesei ◽  
Cellulase Gene ◽  
Transcript Levels ◽  
Content Type ◽  
Camp Pathway ◽  
Kinase A

ABSTRACTThe cyclic AMP (cAMP) pathway represents a central signaling cascade with crucial functions in all organisms. Previous studies ofTrichoderma reesei(anamorph ofHypocrea jecorina) suggested a function of cAMP signaling in regulation of cellulase gene expression. We were therefore interested in how the crucial components of this pathway, adenylate cyclase (ACY1) and cAMP-dependent protein kinase A (PKA), would affect cellulase gene expression. We found that both ACY1 and PKA catalytic subunit 1 (PKAC1) are involved in regulation of vegetative growth but are not essential for sexual development. Interestingly, our results showed considerably increased transcript abundance of cellulase genes in darkness compared to light (light responsiveness) upon growth on lactose. This effect is strongly enhanced in mutant strains lacking PKAC1 or ACY1. Comparison to the wild type showed that ACY1 has a consistently positive effect on cellulase gene expression in light and darkness, while PKAC1 influences transcript levels of cellulase genes positively in light but negatively in darkness. A function of PKAC1 in light-modulated cellulase gene regulation is also reflected by altered complex formation within thecel6a/cbh2promoter in light and darkness and in the absence ofpkac1. Analysis of transcript levels of cellulase regulator genes indicates that the regulatory output of the cAMP pathway may be established via adjustment of XYR1 abundance. Consequently, both adenylate cyclase and protein kinase A are involved in light-modulated cellulase gene expression inT. reeseiand have a dampening effect on the light responsiveness of this process.

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