Phylogenetic-Relationships of the Marsupial Mole, Notoryctes-Typhlops (Marsupialia, Notoryctidae)

1991 ◽  
Vol 39 (5) ◽  
pp. 529 ◽  
Author(s):  
M Westerman
Keyword(s):  
Phylogenetic Relationships ◽  
Single Copy ◽  
Morphological Characters ◽  
Phylogenetic Position ◽  
Entire Genome ◽  
Dna Hybridisation ◽  
Hybridisation Technique ◽  
Copy Dna ◽  
Serological Data ◽  
Single Copy Dna

The phylogenetic position of the marsupial mole, Notorcytes typhlops, has been difficult to ascertain with morphological characters because of its highly specialised fossorial lifestyle. On the basis of serological data, Kirsch (1977b) suggested that this species was sufficiently different from other marsupials to warrant placing it in its own suborder. Using the DNA-DNA hybridisation technique on single-copy DNA to assess sequence differences over the entire genome, I confirm that N. typhlops is not closely related to any other marsupial family, and warrants placement in its own order.

Relations Among Ringtail Possums (Marsupialia, Pseudocheiridae) Based on Dna-Dna Hybridization

1992 ◽  
Vol 40 (4) ◽  
pp. 423 ◽  
Author(s):  
MS Springer ◽  
GM Mckay ◽  
KP Aplin ◽  
JAW Kirsch
Keyword(s):  
Dna Hybridization ◽  
Phylogenetic Analyses ◽  
Single Copy ◽  
Chromatography Method ◽  
Dna Evolution ◽  
Dna Hybridisation ◽  
Close Relationship ◽  
Copy Dna ◽  
The Stability ◽  
Single Copy Dna

Comparison among eight pseudocheirid species and two outgroup petaurids were made by means of the hydroxyapatite chromatography method of DNA hybridisation. Matrices of DELTAT(m) and DELTAT(m)H-C values were analysed with the FITCH algorithm in Felsenstein's PHYLIP (Version 3.3). Jackknifing and bootstrapping were applied to determine the stability of resulting topologies. All the phylogenetic analyses produced trees that support (1) the monophyly of the Pseudocheirus herbertensis complex, (2) the monophyly of Pseudocheirus, (3) a close relationship between Hemibelideus and Petauroides, and (4) a close relationship between Pseudochirops archeri and Pseudochirops cupreus. Rates of single-copy DNA evolution are slightly faster in Pseudocheirus, Hemibelideus, and Petauroides than in Pseudochirops. Hybridisation evidence also provides a framework for understanding the timing of the pseudocheirid radiation and suggests that the divergence between extant genera dates back to about 36 million years ago.

A Partial Classification of Waterfowl (Anatidae) Based on Single-Copy DNA

The Auk ◽  
1988 ◽  
Vol 105 (3) ◽  
pp. 452-459 ◽  
Author(s):  
Cort S. Madsen ◽  
Kevin P. McHugh ◽  
Siwo R. de Kloet
Keyword(s):  
Dna Hybridization ◽  
Phylogenetic Relationships ◽  
Single Copy ◽  
The Other ◽  
Data Matrix ◽  
Partial Classification ◽  
Close Relationship ◽  
Copy Dna ◽  
Single Copy Dna

Abstract Single-copy DNA-DNA hybridization was used to establish phylogenetic relationships among 13 species of waterfowl (Anatidae) chosen from 10 tribes. On the basis of UPGMA clustering of Delta Tm distances, we suggest that the tribes Anatini, Aythyini, Tadornini, Mergini, and Cairinini diverged more recently than the Anserini, Stictonettini, Oxyurini, Dendrocygnini, and Anseranatini. The Freckled Duck (Stictonetta naevosa, tribe Stictonettini) is only distantly related to the other Anatidae. Presumably the lineage diverged very early. The sheldgeese (tribe Tadornini) and the true geese (Anserini) are only remotely related. The Oxyurini, considered to be in the subfamily Anatinae, is remotely related to the other Anatidae. The Dendrocygnini form an isolated tribe with no close relationship to the swans and geese (subfamily Anserinae). We found that the screamers (Anhimidae) are distantly related to the Anatidae. A method to estimate missing cells in a data matrix of pairwise distances is presented.

Human XX males with Y single-copy DNA fragments

Nature ◽  
1984 ◽  
Vol 307 (5947) ◽  
pp. 172-173 ◽  
Author(s):  
Georges Guellaen ◽  
Myriam Casanova ◽  
Colin Bishop ◽  
Danielle Geldwerth ◽  
Gabriel Andre ◽  
...  
Keyword(s):  
Single Copy ◽  
Dna Fragments ◽  
Xx Males ◽  
Copy Dna ◽  
Single Copy Dna

scholarly journals Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.

Genetics ◽  
1991 ◽  
Vol 127 (4) ◽  
pp. 681-698 ◽  
Author(s):  
A J Link ◽  
M V Olson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Physical Map ◽  
Single Copy ◽  
Restriction Endonucleases ◽  
Yeast Genome ◽  
Hybridization Probes ◽  
Copy Dna ◽  
Single Copy Dna

Abstract A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.

High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets

2008 ◽  
Vol 80 (10) ◽  
pp. 3522-3529 ◽  
Author(s):  
Palani Kumaresan ◽  
Chaoyong James Yang ◽  
Samantha A. Cronier ◽  
Robert G. Blazej ◽  
Richard A. Mathies
Keyword(s):  
High Throughput ◽  
Dna Amplification ◽  
Single Copy ◽  
Cell Analysis ◽  
Copy Dna ◽  
Single Copy Dna

Electron microscopy of Achlya deoxyribonucleic acid sequence organization

1981 ◽  
Vol 1 (2) ◽  
pp. 136-143
Author(s):  
M Pellegrini ◽  
W E Timberlake ◽  
R B Goldberg
Keyword(s):  
Dna Sequences ◽  
Deoxyribonucleic Acid ◽  
Microscopic Analysis ◽  
Single Copy ◽  
Electron Microscopic ◽  
Sequence Organization ◽  
Double Stranded Dna ◽  
Copy Dna ◽  
Gene 32 ◽  
Single Copy Dna

Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the highly and moderately repetitive sequence clusters. We used the gene 32 protein-ethidium bromide technique for visualizing the DNA molecules, a procedure which provides excellent contrast between single- and double-stranded DNA regions. Long (greater than 6-kilobase) DNA fragments were isolated after reannealing to two different repetitive C0t values, and the renatured structures were then visualized in an electron microscope. Our results showed that the inverted repeat sequences were short (0.5 kilobase, number-average) and separated by nonhomologous DNA of various lengths. These pairs of sequences were not clustered within the genome. Both highly repetitive and moderately repetitive DNA sequences were organized as tandem arrays of precisely paired, regularly repeating units. No permuted clusters of repeating sequences were observed, nor was there evidence of interspersion of repetitive with single-copy DNA sequences in the Achlya genome.

scholarly journals Genomic distribution of repeated and single copy DNA sequences inPenicillium funiculosum

1987 ◽  
Vol 40 (2-3) ◽  
pp. 315-319 ◽  
Author(s):  
N.A. Sahasrabudhe ◽  
M.N. Sainani ◽  
V.S. Gupta ◽  
P.K. Ranjekar
Keyword(s):  
Dna Sequences ◽  
Single Copy ◽  
Genomic Distribution ◽  
Copy Dna ◽  
Single Copy Dna
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