The Litoria-Ewingi Complex (Anura, Hylidae) in South-Eastern Australia .9. Variation in Mitochondrial-Dna Across a Narrow Zone of Hybridization Between Litoria-Ewingi and Litoria-Paraewingi

1990 ◽  
Vol 38 (1) ◽  
pp. 53 ◽  
Author(s):  
SL Dennington
Keyword(s):  
Mitochondrial Dna ◽  
Hybrid Zone ◽  
Enzyme Analysis ◽  
Advertisement Call ◽  
Restriction Enzyme Analysis ◽  
Southern Limit ◽  
Allopatric Populations ◽  
South Eastern ◽  
Eastern Australia ◽  
South Eastern Australia

The mitochondrial DNA (mtDNA) of Litoria ewingi and L. paraewingi, two species of hylid frog occurring in south-eastern Australia, was investigated by restriction enzyme analysis. Clear diagnostic differences between allopatric populations of these two species were shown in several of the fragment patterns. MtDNA haplotypes of individuals from populations along a transect across the southern hybrid zone between L. ewingi and L. paraewingi were identified. Four individuals from these hybrid populations had inherited a unique haplotype of mtDNA. The width of the hybrid zone based on variation in mtDNA, is at least 5 km. The southern limit of introgression of mtDNA typical of L. paraewingi coincides with the southern limit based on call variation. However, mtDNA typical of L. ewingi was not found as far north as expected by other criteria. The relationship between mtDNA haplotype and structure of advertisement call in individuals from populations in the hybrid zone was analysed. The species-specificity of mtDNA established for allopatric populations held for all but two of the ten recorded putative parental males. Surprisingly, three of the four hybrids based on call structure had inherited mtDNA characteristic of L. ewingi.

Geographic variation in the advertisement call of Crinia signifera (Anura:Myobatrachidae) on Kangaroo Island and across southern south-eastern Australia

2008 ◽  
Vol 56 (4) ◽  
pp. 223 ◽  
Author(s):  
Murray J. Littlejohn
Keyword(s):  
Geographic Variation ◽  
Pulse Number ◽  
Advertisement Call ◽  
Regional Study ◽  
South Eastern ◽  
Island Sample ◽  
Eastern Australia ◽  
Continental Islands ◽  
Two Populations ◽  
South Eastern Australia

The male advertisement call of anuran amphibians has a major role in mate choice, and regional variation in this attribute can act as an indicator of speciation and a marker for genetic differentiation. As part of a regional study of geographic variation in the male advertisement call of Crinia signifera across south-eastern Australia and adjacent larger continental islands, samples of advertisement calls from two populations on Kangaroo Island and two populations on the adjacent Fleurieu Peninsula were compared. Four call attributes were considered: pulse number, call duration, pulse rate and dominant frequency. Pulse number is considered the most reliable for comparative purposes because it is not influenced by effective temperature or audio recording and analysis. The two island populations (central and eastern, ~24 km apart) differ significantly in pulse number, with contact but no overlap of interquartile ranges. The eastern sample differs markedly from those on the nearby Fleurieu Peninsula – which are both similar to the more distant central island sample. Geographic variation in pulse number in these four samples and 11 others from two recent publications is then interpreted in the light of land bridges and lower temperatures of the late Pleistocene and early Holocene.

Allozyme and mitochondrial DNA analysis of carp, Cyprinus carpio L., from south-eastern Australia

1999 ◽  
Vol 50 (3) ◽  
pp. 253 ◽  
Author(s):  
Karyn M. Davis ◽  
Patricia I. Dixon ◽  
John H. Harris
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Cyprinus Carpio ◽  
Dna Analysis ◽  
Restriction Enzymes ◽  
South Eastern ◽  
Eastern Australia ◽  
Carp Cyprinus Carpio ◽  
Allozyme Loci ◽  
South Eastern Australia

Carp (Cyprinus carpio L.) were introduced to Australia on at least three occasions over the past 100 years. These introductions were to the Prospect Reservoir, Sydney (1907), the Murrumbidgee Irrigation Area, New South Wales (NSW) (1940s), and to Boolarra, Victoria (1960). Koi, a colourful variety of carp, have been introduced to several areas as well. Carp are now widely spread throughout south-eastern Australia. This study aimed to investigate genetic variation of carp in south-eastern Australia. Carp from several localities were examined at seven polymorphic allozyme loci and with three restriction enzymes detecting polymorphic sites in mitochondrial DNA. Three composite mtDNA haplotypes were found. Haplotype 1 was spread throughout the study area, occurring in 72% of all individuals examined. Haplotype 2 was localized to south-western NSW and occurred in 4% of individuals. Haplotype 3 was found in the Australian Capital Territory and Tasmania and accounted for 24% of individuals. Little genetic variation within and among carp populations was observed in the mitochondrial DNA data. The allozyme data showed greater variation within populations than did the mitochondrial DNA data.

Preliminary investigation of mitochondrial DNA variation in jack mackerel (Trachurus declivis, Carangidae) from south-eastern Australian waters

1994 ◽  
Vol 45 (4) ◽  
pp. 495 ◽  
Author(s):  
AJ Smolenski ◽  
JR Ovenden ◽  
RWG White
Keyword(s):  
Mitochondrial Dna ◽  
New South ◽  
Preliminary Investigation ◽  
New South Wales ◽  
Heart Tissue ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Jack Mackerel ◽  
South Eastern ◽  
South Wales

A preliminary investigation of the genetic structure of jack mackerel (Trachurus declivis, Carangidae) in south-eastern Australian waters was conducted by using restriction enzyme analysis of mitochondrial DNA (mtDNA). Seventy-five jack mackerel samples were collected from two localities: off Eden in New South Wales and off south-eastern Tasmania. Fish were sampled twice at each locality. Mitochondrial DNA was extracted from developing ovary tissue and heart tissue. The 75 jack mackerel samples were screened with 11 six-base and four four-base enzymes. The level of genetic diversity in jack mackerel was low. The six-base enzyme analysis found limited evidence of reproductive isolation between New South Wales and Tasmanian samples of jack mackerel. However, an analysis of a subset of 42 jack mackerel with four four-base enzymes found evidence of temporal differences in the mtDNA diversities of two Tasmanian samples of jack mackerel.

The Litoria ewingi Complex (Anura: Hylidae) in South-Eastern Australia VII.* Mating Call Structure and Gentic Compatibility across a Narrow Hybrid Zone between L. ewingi and L. parawingi

1983 ◽  
Vol 31 (2) ◽  
pp. 193 ◽  
Author(s):  
MJ Littlejohn ◽  
GF Watson
Keyword(s):  
Hybrid Zone ◽  
Parental Species ◽  
Allopatric Population ◽  
Minimum Width ◽  
Call Structure ◽  
Allopatric Populations ◽  
South Eastern ◽  
Mating Call ◽  
Eastern Australia

On the basis of the number of pulses per repeated note and of repeated notes per call for individual males, the interaction between Litoria ewingi and L. paraewingi in the region of the Tallarook (northern) transect is interpreted as a transitional hybrid zone, with populations consisting of hybrids and individuals of only one or other parental species. The Tallarook transect thus differs from the Glenburn (southern) transect, which is considered to be an overlap of both parental species together with recombination products. The minimum width of the zone in the area of the Tallarook transect is estimated to be 25 km, compared with 5 km for the Glenburn transect. Levels of partial and total anophthalmia were assessed in progeny from in vitro crosses involving a total of 27 males from both transects whose calls had been recorded and analysed, and females of L. ewingi from adjacent allopatry (a measure of the genetic representation of L. paraewingi). The results are, with one exception, consistent with the mating-call structure of these males. As was found with the Glenburn transect, calls of males of L. paraewingi from an allopatric population on the Tallarook transect close to the zone of interaction are more distinctive from calls of L. ewingi in pulses per repeated note than are those of distant allopatric populations of L. paraewingi. This finding is consistent with the suggestion that the products of reinforcing selection may be accumulating near the zone of interaction.

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