Thermal Acclimation in Several Species of Stored-Grain Beetles.

1981 ◽  
Vol 29 (4) ◽  
pp. 483 ◽  
Author(s):  
DE Evans

The chill-coma temperatures, oxygen consumption at several constant temperatures, and fresh weights of adults of Cryptolestes ferrugineus (Steph.), Oryzaephilus surinamensis (L.), Rhyzopertha dominica (F.) and Tribolium castaneum (Hbst.) were determined before and after acclimation for 14 days at 15 deg C in the laboratory in Australia. Acclimation lowered the mean chill-coma temperatures of all species, but most in O. surinamensis. The oxygen consumption of insects that were free to move and of insects restrained during respirometry was reduced by acclimation. The curves relating respiration rate and temperature were shifted downwards by cold acclimation, but its effect on their slopes (i.e. on temperature sensitivity) varied between and within species. Similarly, the relative proportions of the change in the consumption rates of free insects acclimated at 15 deg C that were attributable to locomotor activity or to resting metabolism varied considerably. The weights of C. ferrugineus and R. dominica were increased by cold acclimation, but those of the other 2 species were not.

1980 ◽  
Vol 20 (103) ◽  
pp. 234 ◽  
Author(s):  
ER Sinclair ◽  
M Bengston

In a survey of 60 grain farms on the Darling Downs, Queensland, during 1977-78, the flat grain beetles Cryptolestes spp., which are germ feeders in stored grains, were found on 39 of the farms and in 15% of the 483 samples taken. Ninety percent of infestations involving Cryptolestes spp. were found in bag or bulk stores of seed and feed grain, where they were usually associated with heavy infestations of the three more common stored grain pests, the rice weevil Sitophilus oryzae (L.), the lesser grain borer Rhyzopertha dominica (F.), and the red flour beetle Tribolium castaneum (Herbest.). Three species of Cryptolestes were detected: C. pusillus (Schonherr) (45.6% of farms surveyed), C. ferrugineus (Steph.) (28.1 %), and C. pusilloides (Steel and Howe) (15.8%). During the farm survey, samples were also collected from 8 of the 11 central storages reported infested with Cryptolestes spp. C. ferrugineus was present in each of these samples, C. pusillus in only one, whilst C. pusilloides was not found. The three species were tested for resistance to malathion and fenitrothion, the most commonly used grain-protectant chemicals, by exposing adults to treated wheat. C. pusillus and C. pusilloides were susceptible to both malathion and fenitrothion (LC99.9 < 1 mg kg-1). C. ferrugineus, whilst susceptible to fenitrothion (LC99.9 < 1 mg kg-1) was resistant to malathion, with LC99.9 of 300-400 mg kg-1 compared with the recommended dose of 18 mg kg -1. Although C. pusillus was the most common species on farms, it was concluded that C. ferrugineus was the most important overall because it is biologically better suited to the grain in central storages, which is typically hot (>30�C) and of low moisture content (< 12% for wheat).


1982 ◽  
Vol 22 (115) ◽  
pp. 127 ◽  
Author(s):  
ER Sinclair

Three surveys were undertaken to obtain estimates of the numbers of insects in stored products on farms on the Darling Downs, Queensland. In one survey, 57 farms were sampled once during 1977-1 978. In another, samples were taken at four-weekly intervals from three farms throughout 1977 and in the third, 16 of the first 57 farms were revisited in 1979. Samples were taken from grain stored for farm use, or grain spills and residues. Live adult insects were removed by mechanical sieving, counted and identified. The mean amounts of grain per farm, estimated visually, were 950 kg of bulk and bagged stocks of feed and seed grain, 270 kg of grain spills, 65 kg in auger boots, 100 kg in trucks and in headers, and 75 kg in other residues. Modal amounts were less, being in the range of 0-100 kg per farm for bagged and bulk grain, and spills on earthen floors, and 0-50 kg for other residues. Sitophiius oryzae (L.) (the rice weevil), Rhyzopertha dominica (F.) (the lesser grain borer), the Cryptolestes spp, group (flat grain beetles), and Trifolium castaneum (Herbst.) (rust-red flour beetle) were the most common pests, being 46.5%, 20.9%, 10.8% and 7.3% of the 52 330 insects found in the first two surveys. These four pests were found on 89.3%, 87.5%, 62.5% and 91.1% of the 57 farms in the first survey. The mean farm populations of these four pests combined was estimated at 360 000 adults (with a modal population per farm in the range of 0-50 000) of which approximately 88% were in bulk and bagged stocks of grain. However, this mean was inflated by a small number of farms having very large populations. An estimated 54% of farms had less than 50 000 adult pests. The second survey showed that insect populations peaked in late autumn, and reached a minimum in early summer.


2019 ◽  
Vol 27 ◽  
pp. 11-21
Author(s):  
AC Sarker ◽  
W Islam ◽  
S Parween

The hemipteran predator, Xylocoris flavipes (Reuter) predates the eggs, larvae and pupae of Rhyzopertha dominica (F.) in storage condition and checks their population in considerable level. The nymphs of 1st up to 5th instar and adults of X. flavipes were found efficient to survive on eggs, larvae of 1st up to 4th instar and pupae of R. dominica. The mean duration of developmental period through five nymphal instars on eggs, larvae of 1st up to 4th instars and pupae were 18 ± 1.00, 20 ± 0.58, 16 ± 2.00, 14 ± 1.15, 12 ± 1.15 and 13 ± 0.58 days in R. dominica. The adult female X. flavipes survived longer than the male. Average consumption rates of each nymph of 1st up to 5th instar and adult stage of X. flavipes were found highest on eggs, 1st and 2nd instar larvae but lowest on 4th instar larvae and pupae. The female predator always consumed more individuals than the male. Average survivability rates of nymphs of 1st up to 5th instar and adults were maximum on 1st and 2nd instar larvae and minimum on 4th instar larvae and pupae. The size of the female predator was found larger than the male at all the stages. Based on the ratio 1:1, sex ratio was the best (male and female almost equal in number) on 1st and 2nd instar larvae comparatively than that of other stages. Developmental period, adult longevity, consumption rate, survivability rate, size and sex ratio of X. flavipes were found always significant (p<0.001) in different life stages of R. dominica. This study reveals that mass culture of X. flavipes can be established on R. dominica in the laboratory to get easy and abundant supply of the bug as an agent of biological control. J. bio-sci. 27: 11-21, 2019


1984 ◽  
Vol 24 (125) ◽  
pp. 260 ◽  
Author(s):  
ER Sinclair ◽  
J Alder

One hundred unsexed adults of each of the following coleopterous species were added to 27 .3 kg of clean wheat: Sitophilus oryzae (L.) (rice weevil), Rhyzopertha dominica (F.) (lesser grain borer), Tribolium castaneum (Herbst) (rust-red flour beetle), and the flat grain beetles Cryptolestes pusillus (Schonherr), C. ferrugineus (Stephens), and C. pusilloides (Steele and Howe). For 15 months, measurements were made of population size and emigration from this wheat bulk. The highest populations recorded for each species were: 138 850, 65 680, 9 060, 47 000, 15 530, 11 940, respectively, and total emigrant numbers during the period were: 774 682, 278 094, 123 784, 335 588, 39 070, 9 352, respectively. Such high numbers of emigrants in relation to relatively low source populations emphasize the significance of small amounts of infested grain as sources of infestation of large grain bulks, and hence the importance of grain hygiene. The proportion of females in the populations of four species changed significantly from 0.50 as populations developed. Emigration behaviour was influenced most commonly by temperature, insect numbers, and wheat age, but these factors did not act uniformly on all species. Negligible numbers of emigrants returned to the food source. Low numbers caught in food traps away from the source were consistent with these not being attractive but traps near the shed walls caught greater numbers than expected.


1976 ◽  
Vol 35 (03) ◽  
pp. 712-716 ◽  
Author(s):  
D. Del Principe ◽  
G Mancuso ◽  
A Menichelli ◽  
G Maretto ◽  
G Sabetta

SummaryThe authors compared the oxygen consumption in platelets from the umbilical cord blood of 36 healthy newborn infants with that of 27 adult subjects, before and after thrombin addition (1.67 U/ml). Oxygen consumption at rest was 6 mμmol/109/min in adult control platelets and 5.26 in newborn infants. The burst in oxygen consumption after thrombin addition was 26.30 mμmol/109/min in adults and 24.90 in infants. Dinitrophenol did not inhibit the burst of O2 consumption in platelets in 8 out of 10 newborn infants, while the same concentration caused a decrease in 9 out of 10 adult subjects. Deoxyglucose inhibited the burst in O2 consumption in newborn infant and adult platelets by about 50%. KCN at the concentration of 10−4 M completely inhibited basal oxygen consumption but did not completely inhibit the burst after thrombin. At the concentration of 10−3 M, it inhibited both basal O2 consumption and the burst in infants and adult subjects.


2017 ◽  
Vol 4 (2) ◽  
pp. 14
Author(s):  
Putri Megasari

Hepatitis has become a health problem in the world. The hepatitis virus infected many people. According to the teacher of MTsN 02 Bondowoso more than 20 students have hepatitis A viral infection. The purpose of this research was to know the differences of students' knowledge about hepatitis A before and after counseling in MTsN 02 Bondowoso 2015. This study used pre-experimental (pre-post test design). This study used stratified random sampling technique, 127 students from 270 sample involved this research,and 143 students was excluded. We used questionnaires to collect data. The results showed that the mean value of the students 'knowledge about hepatitis A before counseling in MTsN 02 Bondowoso 2015 was 83.96 with the lowest value of 37.5 and the highest value was 100. The mean value of the students' knowledge about hepatitis A after counseling in MTsN 02 Bondowoso 2015 was 93.21 with the lowest value waf 62.5 and the highest value was 100. Paired t test showed that t (-9.07) > t table (1.98), the null hypothesis (H0) was rejected. There was a difference between students' knowledge about hepatitis A before and after counseling in MTsN 02 Bondowoso 2015. This study showed that routine counseling by healthcare provider was important to prevent hepatitis A infection.; Keywords: counseling, knowledge of students, hepatitis


2003 ◽  
Vol 128 (1) ◽  
pp. 17-26 ◽  
Author(s):  
David J. Kay ◽  
Richard M. Rosenfeld

OBJECTIVE: The goal was to validate the SN-5 survey as a measure of longitudinal change in health-related quality of life (HRQoL) for children with persistent sinonasal symptoms. DESIGN AND SETTING: We conducted a before and after study of 85 children aged 2 to 12 years in a metropolitan pediatric otolaryngology practice. Caregivers completed the SN-5 survey at entry and at least 4 weeks later. The survey included 5 symptom-cluster items covering the domains of sinus infection, nasal obstruction, allergy symptoms, emotional distress, and activity limitations. RESULTS: Good test-retest reliability ( R = 0.70) was obtained for the overall SN-5 score and the individual survey items ( R ≥ 0.58). The mean baseline SN-5 score was 3.8 (SD, 1.0) of a maximum of 7.0, with higher scores indicating poorer HRQoL. All SN-5 items had adequate correlation ( R ≥ 0.36) with external constructs. The mean change in SN-5 score after routine clinical care was 0.88 (SD, 1.19) with an effect size of 0.74 indicating good responsiveness to longitudinal change. The change scores correlated appropriately with changes in related external constructs ( R ≥ 0.42). CONCLUSIONS: The SN-5 is a valid, reliable, and responsive measure of HRQoL for children with persistent sinonasal symptoms, suitable for use in outcomes studies and routine clinical care.


Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 233
Author(s):  
Ali A. Badawy ◽  
Nilly A. H. Abdelfattah ◽  
Salem S. Salem ◽  
Mohamed F. Awad ◽  
Amr Fouda

Herein, CuO-NPs were fabricated by harnessing metabolites of Aspergillus niger strain (G3-1) and characterized using UV–vis spectroscopy, XRD, TEM, SEM-EDX, FT-IR, and XPS. Spherical, crystallographic CuO-NPs were synthesized in sizes ranging from 14.0 to 47.4 nm, as indicated by TEM and XRD. EDX and XPS confirmed the presence of Cu and O with weight percentages of 62.96% and 22.93%, respectively, at varied bending energies. FT-IR spectra identified functional groups of metabolites that could act as reducing, capping, and stabilizing agents to the CuO-NPs. The insecticidal activity of CuO-NPs against wheat grain insects Sitophilus granarius and Rhyzopertha dominica was dose- and time-dependent. The mortality percentages due to NP treatment were 55–94.4% (S. granarius) and 70–90% (R. dominica). A botanical experiment was done in a randomized block design. Low CuO-NP concentration (50 ppm) caused significant increases in growth characteristics (shoot and root length, fresh and dry weight of shoot and root, and leaves number), photosynthetic pigments (total chlorophylls and carotenoids), and antioxidant enzymes of wheat plants. There was no significant change in carbohydrate or protein content. The use of CuO-NPs is a promising tool to control grain insects and enhance wheat growth performance.


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