Studies on the vaginal mucusof the marsupial Trichosurus vulpecula

1971 ◽  
Vol 19 (1) ◽  
pp. 19 ◽  
Author(s):  
RL Hughes ◽  
JC Rodger
Keyword(s):  
Body Weight ◽  
Dry Matter ◽  
Follicle Development ◽  
Trichosurus Vulpecula ◽  
Follicle Growth ◽  
Vaginal Smears ◽  
Low Concentrations ◽  
Oestradiol Benzoate ◽  
Neutral Sugars ◽  
Sperm Reservoir

The vaginal mucus of T. vulpecula is secreted by the simple columnar epithelium of the vaginal cul-de-sac and upper portion of the lateral vaginal canals. Vaginal mucus was present in measurable quantities for a period of only 3-4 days: from the commencement of oestrus (as judged by vaginal smears) until about half a day after ovulation. Over this period the volume of mucus in the cul-de-sac rose rapidly from practically nil to about 3.5 ml at ovulation. Following ovulation, the volume very soon returned to its former level. Treatment of anoestrous animals with doses of oestradiol benzoate at 40ug/kg body weight daily for 2 or 4 days, and 20 or 40 ug/kg body weight for 8 days, resulted in secretion of mucus in similar quantities to that found in oestrous animals. Preliminary studies of the physical properties (density, percentage dry matter, viscosity, and "stickiness") of the vaginal mucus of T. vulpecula revealed few trends that could be causally related to follicle development, oestrus, and ovulation. However, the pH appeared to follow a trend with regard to follicle development. It was found to fall from 7-8 to 6 at the end of follicle growth. The histochemical findings suggest that the vaginal mucus of T. vulpecula is a carbohydrate-protein com- plex, containing neutral sugars and acidic mucopolysaccharides. The acidic mucopolysaccharides are mainly carboxylated; however, low concentrations of sulphated mucopolysaccharide are also evident. The presence of appreciable quantities of free vaginal mucus in the vaginal cul-de-sac between oestrus and ovulation suggests that it is likely to be functionally important in the maintenance of a sperm reservoir.

DEVELOPMENT OF OVARIAN AUTOGRAFTS ON THE GREATER OMENTUM IN SPAYED HYPOPHYSECTOMIZED RATS AND IN CASTRATES TREATED WITH METHALLIBURE, GONADOTROPHINS AND OESTRADIOL BENZOATE

1972 ◽  
Vol 70 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Artur Ber
Keyword(s):  
Body Weight ◽  
Greater Omentum ◽  
Tween 20 ◽  
Control Groups ◽  
Complete Cessation ◽  
Oestradiol Benzoate ◽  
Hypophysectomized Rats ◽  
Growth Promoting

ABSTRACT Hypophysectomy was performed at the same time as ovariectomy in 19 rats aged 6 weeks; in addition a fragment of the animal's own ovary was implanted into the greater omentum. The graft took but there was complete cessation of the growth of the implant and a decrease in body and uterine weights as compared to 15 ovariectomized controls of the same age killed after one month. This shows that the pituitary is indispensable for the growth of ovarian implants but not for their take. In 84 spayed rats aged 3 weeks with ovarian implants, methallibure (6 mg/day in a 0.2% solution of Tween 20) was given by gavage during one month. Some of them were treated with PMSG (20 IU/day), HCG (20 IU/day) or with oestradiol benzoate (0.05 mg/day) only or in combinations. As controls, there were 74 spayed rats with ovarian implants, seven of which received Tween 20, and 67 were untreated. No differences were found between the two control groups. Methallibure alone caused arrest of the development of the implants and uteri and a decrease in body weight. In the methallibure treated animals HCG stimulated the growth of the implant which was, however, smaller than that in the controls, while PMSG restored the weight of the implant even above that of the control rats. Oestradiol benzoate caused a further diminution in the size of the implants but augmented considerably the influence of HCG on the implants. It had no effect on the weight of the implant in rats treated with PMSG. It is concluded that FSH is the principal implant growth-promoting factor and that oestrogens appear to act in two directions. They decrease the gonadotrophin output from the pituitary thus inhibiting the growth of the implants, but in the presence of gonadotrophins they act directly on the implants, promoting their development.

Digestibility of Nutrients as Influenced by Supplementation of Exogenous Fibrolytic Enzymes in Dry Non-pregnant Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
P. M, Lunagariya ◽  
R. S. Gupta ◽  
S. V. Shah ◽  
Y. G. Patel
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dairy Cows ◽  
Dry Matter ◽  
Body Weight Gain ◽  
The Body ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Fibrolytic Enzymes ◽  
Nitrogen Free Extract

The study was planned to evaluate the effect of exogenous fibrolytic enzymes (EFE) supplementation for 56 days @ 240 mg/kg total mixed ration (TMR) on digestibility of dry matter and nutrients in dairy cows. Six dry non-pregnant cows were assigned in each treatment with and without EFE. The digestibility trial of seven days was conducted after 49 days of feeding. Dry matter and nutrients intake of cows was not influenced by EFE. The supplementation of EFE had improved digestibility of dry matter, organic matter, crude fiber, neutral detergent fiber, cellulose (p less than 0.01), as well as digestibility of nitrogen-free extract and acid detergent fiber, was also higher (pless than 0.05). The body weight gain of cows was higher on the supplementation of EFE in TMR. The study concluded that feeding exogenous fibrolytic enzymes (240 mg/kg) supplemented TMR improved digestibility of dry matter and nutrients, which was reflected as higher body weight gain in dry non-pregnant Gir and crossbred dairy cows.

scholarly journals Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

Toxins ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 232
Author(s):  
Antonio Gallo ◽  
Francesca Ghilardelli ◽  
Alberto Stanislao Atzori ◽  
Severino Zara ◽  
Barbara Novak ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Dry Matter ◽  
Kojic Acid ◽  
Corn Silage ◽  
Fungal Metabolites ◽  
Fusarium Mycotoxins ◽  
Low Concentrations ◽  
Fermentation Quality ◽  
Low Levels

Sixty-four corn silages were characterized for chemicals, bacterial community, and concentrations of several fungal metabolites. Silages were grouped in five clusters, based on detected mycotoxins, and they were characterized for being contaminated by (1) low levels of Aspergillus- and Penicillium-mycotoxins; (2) low levels of fumonisins and other Fusarium-mycotoxins; (3) high levels of Aspergillus-mycotoxins; (4) high levels of non-regulated Fusarium-mycotoxins; (5) high levels of fumonisins and their metabolites. Altersetin was detected in clusters 1, 3, and 5. Rugulusovin or brevianamide F were detected in several samples, with the highest concentration in cluster 3. Emodin was detected in more than 50.0% of samples of clusters 1, 3 and 5, respectively. Kojic acid occurred mainly in clusters 1 and 2 at very low concentrations. Regarding Fusarium mycotoxins, high occurrences were observed for FB3, FB4, FA1, whereas the average concentrations of FB6 and FA2 were lower than 12.4 µg/kg dry matter. Emerging Fusarium-produced mycotoxins, such as siccanol, moniliformin, equisetin, epiequisetin and bikaverin were detected in the majority of analyzed corn silages. Pestalotin, oxaline, phenopirrozin and questiomycin A were detected at high incidences. Concluding, this work highlighted that corn silages could be contaminated by a high number of regulated and emerging mycotoxins.

Digestive Response of Rainbow Trout, Salmo gairdneri, to Pellet Diets

1969 ◽  
Vol 26 (7) ◽  
pp. 1801-1812 ◽  
Author(s):  
John T. Windell ◽  
David O. Norris ◽  
James F. Kitchell ◽  
James S. Norris
Keyword(s):  
Body Weight ◽  
Rainbow Trout ◽  
Dry Matter ◽  
Laboratory Experiments ◽  
Corn Oil ◽  
Saturated Fat ◽  
Methyl Cellulose ◽  
Salmo Gairdneri ◽  
Gastric Evacuation ◽  
Gastric Contents

Quantitative data are presented for three laboratory experiments concerning rate of gastric evacuation of pellets (fed in gelatin capsules) and pellet components. Rainbow trout, Salmo gairdneri, acclimated to a constant water temperature of 15 C were killed 12 hr after consuming an experimental meal. Subtraction of amount of dry matter remaining at autopsy from dry matter consumed yielded amount of food digested or evacuated or both, from the stomach per unit time.Meals of encapsulated pellet were evacuated from the stomach more rapidly (65.8% decrease in 12 hr) than encapsulated corn oil (42.6%), gelatin (50.8%), a combination of corn oil and gelatin (47.3%), saturated fat (28.8%), or methyl cellulose (50.3%).Groups of fish consuming five capsules equal to approximately 0.65 % of their body weight evacuated 35.9, 45.1, 64.2, 95.5, and 100% at intervals after killing from 6 to 36 hr, respectively. Similar groups consuming seven capsules equal to approximately 1.0% of their body weight evacuated 23.7, 57.9, 70.5, and 86.6% at intervals after killing from 4 to 20 hr, respectively.Ten groups of trout consuming amounts of dry matter equal to 0.24, 0.40, 0.74, 1.11, 1.31, 1.19, 1.59, 1.56, 1.91, and 2.26% of their body weight evacuated 90.7, 81.3, 64.2, 57.9, 56.6, 52.5, 53.4, 51.3, 58.7, and 50.0% in 12 hr, respectively. Gastric motility remains relatively constant once food has entered the stomach. However, when larger meals are fed a greater quantity is evacuated per unit time. This could only be accomplished by changes in volume of gastric contents pumped per peristaltic stroke or number of strokes per unit time.

Changes in ovarian follicle composition with plasma levels of snakes during estrus

1959 ◽  
Vol 197 (2) ◽  
pp. 360-366 ◽  
Author(s):  
Herbert C. Dessauer ◽  
Wade Fox
Keyword(s):  
Body Weight ◽  
Plasma Levels ◽  
Fat Body ◽  
Ovarian Follicle ◽  
Liver Weight ◽  
Plasma Calcium ◽  
Yolk Protein ◽  
Follicle Development ◽  
Early Cleavage ◽  
Liver Changes

The first stage of follicle development was due chiefly to hydration; during the second (deutoplasmic) stage 60 mg of solid were taken up with each 100 mg increase in follicle weight. Plasma calcium and protein P rose near end of hydration stage, remained elevated during deutoplasmic stage, reached extreme levels (max. Ca = 90 mm/l.; protein P = 86 mm/l.) near ovulation, and generally fell to anestrous levels while eggs were in early cleavage. Calcium increased in proportion to protein bound P of both plasma and follicles. During deutoplasmic stage a phospho-lipoprotein, of similar gross composition to yolk protein, appeared in plasma. Liver weight increased during hydration stage, remained elevated throughout deutoplasmic stage and decreased near ovulation. Fat body weight increased with onset of estrus, reached maximum during hydration stage and progressively decreased during deutoplasmic stage. Plasma and liver changes characteristic of estrus were reproduced in fasted male snakes with estradiol injections.

scholarly journals Preovulatory follicle development and ovulation in the brushtail possum (Trichosurus vulpecula) monitored by repeated laparoscopy

Reproduction ◽  
1997 ◽  
Vol 110 (2) ◽  
pp. 361-370 ◽  
Author(s):  
J. L. Crawford ◽  
G. H. Shackell ◽  
E. G. Thompson ◽  
B. J. McLeod ◽  
P. R. Hurst
Keyword(s):  
Brushtail Possum ◽  
Follicle Development ◽  
Trichosurus Vulpecula ◽  
Preovulatory Follicle

EFFECT OF STAGE OF LACTATION AND DIET ON SERUM INSULIN LEVEL AND MONONUCLEAR LEUKOCYTE INSULIN RECEPTOR CHARACTERISTICS IN DAIRY COWS

1987 ◽  
Vol 67 (3) ◽  
pp. 721-733 ◽  
Author(s):  
A. D. KENNEDY ◽  
J. R. INGALLS ◽  
W. M. PALMER ◽  
F. R. TEKPETEY
Keyword(s):  
Body Weight ◽  
Insulin Receptor ◽  
Milk Yield ◽  
Sunflower Seed ◽  
Dry Matter ◽  
Serum Insulin ◽  
Mononuclear Leukocytes ◽  
Mononuclear Leukocyte ◽  
Acid Detergent Fiber ◽  
Stage Of Lactation

Blood was collected from and measurements were made of body weight and ultrasonic backfat (US) in ten 2–6 yr old Holstein cows at 8 wk prepartum, 3 d postpartum and at 4, 6 and 12 wk of lactation. Cows were fed a low-fat (LF) total mixed ration containing 3.0% either extract and 13.6% acid detergent fiber ad libitum until day 15 postpartum when some of the diet concentrate was replaced by whole sunflower seeds (four cows) or sunflower seed hulls, sunflower seed meal and tallow (two cows) to yield diets high in fat (6.7 and 6.9%, respectively) and acid detergent fiber (18.1 and 17.9%, respectively). All diets were formulated to have a NE1 of 7.07 MJ kg−1 dry matter and were isonitrogenous. Milk yield and composition were measured daily during each week of blood collection. Radioimmunoassay for insulin was performed on eight serum samples collected at 0.5-h intervals commencing at 0730 h. Specific cell binding (SB) of insulin as well as number of and affinity constants for high affinity (N1, K1, respectively) and low affinity (N2, K2, respectively) insulin receptors were determined on mononuclear leukocytes (MNL) isolated from 250 mL of whole blood. Throughout the trial the US measurement in cows on the LF diet was greater than in cows on the high-fat (HF) diets. Body weight, dry matter intake and milk yield were similar in HF and LF fed cows. Serum insulin concentration was lower 3 d postpartum than prepartum and had increased by 4 wk of lactation. The increase was greater with HF feeding. Insulin SB to MNL was negatively correlated to body weight and N1 was positively correlated to US measurement. Percent insulin SB to MNL at 12 wk of lactation (8.4 ± 1.6) was lower than that found for all other stages of lactation. There was no effect of diet or stage of lactation on K1 and K2 values. At 4 wk of lactation N2 (1.44 ± 0.15 × 104 sites cell−1) was 100 and 50% greater than at 6 and 12 wk of lactation, respectively. Prepartum N2 (1.21 ± 0.17 × 104) did not differ from N2 at any other stage of lactation. At 4 wk of lactation N1 (5.79 ± 0.73 × 102 sites cell−1) tended (P < 0.056) to be greater than that found for all other stages of lactation. There was no significant (P ≥ 0.05) effect of diet on N1, N2, K1, K2 or SB. Key words: Insulin, receptor, mononuclear leukocyte, lactation, dairy cow

Treated sugarcane bagasse for maintenance of sheep in Brazil

1996 ◽  
Vol 1996 ◽  
pp. 172-172
Author(s):  
A.L. Abdalla ◽  
D.M.S.S. Vitti ◽  
N.L. del Mastro ◽  
M.S. Bueno
Keyword(s):  
Body Weight ◽  
Sugarcane Bagasse ◽  
Present Experiment ◽  
Dry Matter ◽  
Weight Changes ◽  
Corn Straw ◽  
Microbial Synthesis ◽  
Body Weight Changes ◽  
Wool Production

Several treatments for increasing digestibility of sugarcane bagasse (SCB) have been studied in Brazil for many years (Abdalla et al., 1990). Despite some improvements in in situ digestibility obtained with steam and pressure treatments, the SCB has not performed well when given to cattle (Mello Junior, et al., 1989). More recently, irradiation and ammonia has been tested in studies to improve the digestibility of SCB, rice and corn straw, rice and soybean hulls and others rough feed. The purpose of the present experiment was aimed to determine the intake, body weight changes, wool production and microbial synthesis in sheep fed with grass hay (Cynodon dactilon) or SCB treated with 600kGy of radiation and ammonia (20g/kg dry matter (DM)).

scholarly journals Milk yield and composition, feed intake and stocking rate of crossbread cows in tropical grasses managed in a rotational grazing system

2010 ◽  
Vol 39 (7) ◽  
pp. 1548-1557 ◽  
Author(s):  
Nelson Massaru Fukumoto ◽  
Julio Cesar Damasceno ◽  
Fermino Deresz ◽  
Carlos Eugênio Martins ◽  
Antônio Carlos Cóser ◽  
...  
Keyword(s):  
Body Weight ◽  
Milk Yield ◽  
Dry Matter ◽  
Experimental Period ◽  
The Body ◽  
Dry Matter Intake ◽  
Panicum Maximum ◽  
Stocking Rate ◽  
Rotational Grazing ◽  
Grazing System

The objective of this study was to evaluate milk yield and composition, dry matter intake, and stocking rate in pastures with tanzania grass (Panicum maximum cv. Tanzânia), star grass (Cynodon nlemfuensis cv. Estrela-Africana), and marandu grass (Brachiaria brizantha cv. Marandu). The grasses were managed in a rotational grazing system with Holstein x Zebu crossbreed cows, with a 30-day resting period and three days of paddock occupation. The pastures were fertilized with 1,000 kg/ha/year using the 20:05:20 (NPK) formula, split in three applications during the rainy season. It was used a complete random block experimental design with three factors being studied and two replications. In the experiment, four cows/paddock were used and, when it was necessary, regulator animals were added in order to obtain a supply of 7% body weight green forage dry matter. The animals were individually fed concentrate at 2 kg/day during the experimental period. Milk yield did not differ among the three grasses, with values of 9.1; 9.1; and 8.7 kg/cow/day for pastures with tanzania grass, star grass and marandu grass, respectively. Similarly, grass did not affect milk chemical composition. Stocking rate was similar among the three grasses, with values of 4.6; 4.5 and 5.0 UA/ha for tanzania grass, star grass and marandu grass, respectively. The highest dry matter intake was observed for tanzania grass with 2.6% of the body weight while stargrass (2.3%) and marandu grass (2.4%) did not differ among each other. The highest dry matter intake on tanzania grass pasture was not reflected on milk yield per animal. Milk yield and composition and stocking rate are similar among the evaluated grasses.

Corn or soybean hull incorporation into haylage-based backgrounding beef cattle diets: Effect on growth and efficiency during the backgrounding and finishing periods

2007 ◽  
Vol 87 (4) ◽  
pp. 615-621 ◽  
Author(s):  
K. C. Swanson ◽  
M. Ko ◽  
C. J. Mader
Keyword(s):  
Body Weight ◽  
Beef Cattle ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Soybean Hulls ◽  
Feedlot Performance ◽  
Final Body Weight ◽  
Plasma Urea ◽  
Soybean Hull ◽  
Daily Gain

This experiment was conducted to evaluate the effects of corn or soybean hull incorporation into haylage-based diets on backgrounding calf performance and subsequent feedlot performance. Crossbred steers [n = 48, initial body weight (BW) = 303 ± 3.4 kg] were individually fed dietary treatments consisting of: (1) haylage [17.5% crude protein, dry matter (DM) basis; control], (2) haylage + 20% (DM basis) cracked corn, and (3) haylage + 20% (DM basis) soybean hulls during a 112-d backgrounding period. After the backgrounding period, all steers were adapted to a common high moisture corn-based finishing diet and fed until an ultrasound estimated backfat thickness of 7 mm was obtained. During the backgrounding period, steers fed cracked corn or soybean hulls had greater (P < 0.01) average daily gain, dry matter intake and gain:feed and lower (P = 0.05) plasma urea N as compared with controls. However, when finished on a common high-concentrate diet, steers previously fed cracked corn had greater (P < 0.08) average daily gain, final body weight, and longissimus muscle area than those fed soybean hulls, suggesting that source of supplemental energy during the backgrounding period may influence subsequent feedlot performance. Key words: Beef cattle, backgrounding, energy supplementation

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