The fate of isotope-labelled uterine spermatozoa in the mouse post coitum

1965 ◽  
Vol 13 (4) ◽  
pp. 525 ◽  
Author(s):  
BL Reid
Keyword(s):  
Lymph Nodes ◽  
Peritoneal Cavity ◽  
Tritiated Thymidine ◽  
Uterine Wall ◽  
Phagocytic Cells ◽  
Uterine Lumen ◽  
Polymorphonuclear Granulocytes ◽  
Uterine Mucosa ◽  
Needle Track ◽  
Degree Of Degradation

Isotopically labelled sperm was used to investigate the fate of the uterine sperm residue not used in the process of fertilization of the mouse. Portion of the sperm present in the uterus exhibiting a copulation plug was removed and replaced by intrauterine injection of sperm labelled either specifically by tritiated thymidine or non-specifically by exposure to a tritium source. The latter label was found more suitable for tracer use although the results with both methods were qualitatively similar. Seventeen hours after injection label was present in sperm in the lumen, in debris associated with polymorphonuclear granulocytes and monocytes in the lumen, in the epithelial and subepithelial coats of the mucosa and in phagocytic cells of the lower abdominal lymph nodes and spleen. The density of labelling was greatest in the sperm itself then fell away sharply and uniformly in the other sites. Label was present at this time in sperm spilled into the peritoneal cavity via the needle track. Associated with this spillage, label was seen in peritoneal polymorpho- nuclear granulocytes and macrophages, in macrophages of the uterine wall, and in phagocytic cells of the lymph nodes and spleen. The density of labelling was greatest in the sperm itself but the density decline in these other sites was less than in these same sites resulting from sperm retained wholly in the uterine lumen. Labelled sperm was present in all experiments in the vaginal lumen. The relation between the density of labelling and the degree of degradation of the sperm products is discussed and it is reasoned that the female cells are exposed to less degraded sperm products as a result of entry via the peritoneal cavity than entry via the uterine mucosa. This route may be thereby more effective as an antigenic stimulant.

Receptor Binding Inhibitor Suppresses Carcinogenesis of Cervical Cancer by Depressing Levels of FSHR and ERβ in Mice

2019 ◽  
Vol 19 (14) ◽  
pp. 1719-1727
Author(s):  
Zhuandi Gong ◽  
Xiaoyun Shen ◽  
Juan Yang ◽  
Luju Lai ◽  
Suocheng Wei
Keyword(s):  
Cervical Cancer ◽  
Receptor Binding ◽  
Estrogen Receptor Beta ◽  
Initial Study ◽  
Uterine Wall ◽  
Fsh Receptor ◽  
Uterine Lumen ◽  
Maturation Rate ◽  
Endometrial Epithelium ◽  
And Control

Background: FSH Receptor Binding Inhibitor (FRBI) blocked the binding of FSH to FSHR. Our initial study revealed FRBI reduced the maturation rate, enhanced the apoptosis of sheep Cumulus-Oocyte Complex (COCs). Little is known about whether FRBI modulates ERβ and FSHR levels in the normal uterine and cancerous tissues. The present study aimed to evaluate the FRBI effects on the expressions of Estrogen Receptor-beta (ERβ) and FSH receptor (FSHR) in the uteri. Methods: Methods: 150 mice were assigned to FRBI+FSH (COM), FSH and control groups (CG). Mice of COM-1, COM-2 and COM-3 groups were simultaneously intramuscularly injected with 500, 750 and 1000 µg FRBI with 10 IU FSH, respectively for five days. Western blotting and qPCR were utilized to determine the expression of ERβ and FSHR. Results: In comparison with FSH group, uterine lumen and glands of COM groups became narrow. The uterine wall and endometrial epithelium were thinned, and uterine lumen became narrow. Epithelial cells were decreased. Uterine wall thicknesses of COM-1, COM-2 and COM-3 groups were reduced by 6.49%, 14.89% and 15.69% on day 30 as compared with FSH group. Uterine perimetrium thicknesses of COM-1, COM-2 and COM-3 groups were reduced by 16.17%, 17.93% and 19.92% on day 20 in comparison with FSH group. Levels of FSHR mRNAs and proteins of COM-1, COM-2 and COM-3 groups were less than FSH group on days 20 and 30 (P<0.05). ERβ protein of COM-3 group was less than FSH group. Serum estradiol (E2) and FSH concentrations of COM-2 and COM-3 were lower than that of FSH group on day 30. Conclusion: FRBI could decrease UWT and UPT, also block the uterine development, decline expression levels of ERβ and FSHR protein. Additionally, FRBI reduced the secretion of secretion of FSH and E2. Downregulating expression of FSHR and ERβ may be a potential treatment regimen for cervical cancer patients.

A STUDY OF BLASTOCYSTS DURING DELAY AND SUBSEQUENT IMPLANTATION IN LACTATING MICE

1968 ◽  
Vol 42 (3) ◽  
pp. 453-463 ◽  
Author(s):  
ANNE McLAREN
Keyword(s):  
Dna Synthesis ◽  
Thymidine Incorporation ◽  
Tritiated Thymidine ◽  
Uterine Horn ◽  
Uterine Epithelium ◽  
Serial Sections ◽  
Uterine Lumen ◽  
Fresh State

SUMMARY Blastocysts were studied on the 5th and 8th day of pregnancy in lactating mice, in the fresh state, flushed from the uterus, in squash preparations and in serial sections. At the earlier period some mitosis was observed. Tritiated thymidine incorporation studies gave some evidence of DNA synthesis on the 5th and 6th days of pregnancy. By the 8th day the blastocysts were longer, contained more cells, and mitosis had ceased. They were located at the anti-mesometrial end of the uterine lumen, closely apposed to the uterine epithelium, and with their long axes parallel to the long axis of the uterine horn. Implantation could be induced, either by the removal of the litter, or by the injection of an appropriate dose of oestrogen on the 5th or 7th (but not the 4th) day of pregnancy. Both treatments were followed by the appearance of W-bodies in the neighbourhood of the blastocysts, the disappearance of the shed zonae, and the appearance of Pontamine Blue reactivity, oedema of the uterine stroma and formation of the primary decidual zone, in that order.

Characterization of an intraperitoneal ovarian cancer xenograft model in nude rats using noninvasive microPET imaging

2007 ◽  
Vol 17 (2) ◽  
pp. 407-417 ◽  
Author(s):  
C. L. Zavaleta ◽  
W. T. Phillips ◽  
Y. C. Bradley ◽  
L. M. McMANUS ◽  
P. A. Jerabek ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Lymph Nodes ◽  
Peritoneal Cavity ◽  
Imaging Modality ◽  
Xenograft Model ◽  
Tumor Model ◽  
Fdg Uptake ◽  
Tumor Bearing ◽  
Nude Rats ◽  
Biodistribution Data

MicroPET is a noninvasive imaging modality that can potentially track tumor development in nude rats using the radiotracer fluorine 18-fluorodeoxyglucose (18F-FDG). Our goal was to determine whether microPET, as opposed to more invasive techniques, could be used to noninvasively monitor the development of ovarian cancer in the peritoneal cavity of nude rats for monitoring treatment response in future studies. Female nude rats were inoculated intraperitoneally with 36 million NIH:OVCAR-3 cells. Imaging was carried out at 2, 4, 6, or 8 weeks postinoculation. Each rat was fasted overnight and intravenously injected with 11.1 MBq (300 μCi) of 18F-FDG in 0.2 mL of saline. Thirty minutes following injection, the rats were placed in the microPET and scanned for 30 min. After imaging, rats were euthanized for ascites and tissue collection for biodistribution and histopathologic correlation. Standard uptake values (SUVs) of 18F-FDG within the peritoneal cavity were also calculated from regions of interest analysis of the microPET images. MicroPET images showed diffuse increased uptake of 18F-FDG throughout the peritoneal cavity of tumor rats (mean SUV = 4.64) compared with control rats (mean SUV = 1.03). Ascites gathered from tumor-bearing rats had increased 18F-FDG uptake as opposed to the peritoneal fluid collected from control rats. Biodistribution data revealed that the percent injected dose per gram (% ID/g) was significantly higher in tumor-bearing rats (6.29%) than in control rats (0.59%) in the peritoneal lymph nodes. Pathology verified that these lymph nodes were more reactive in tumor-bearing rats. By 6 weeks, some rats developed solid masses within the peritoneum, which could be detected on microPET images and confirmed as tumor by histopathology. 18F-FDG uptake in these tumors at necropsy was 2.83% ID/g. These results correlate with previous invasive laparoscopic studies of the same tumor model and demonstrate that microPET using 18F-FDG is a promising noninvasive tool to localize and follow tumor growth in an intraperitoneal ovarian cancer model.

scholarly journals Comparative histopathology of the lymph nodes, spleen, liver and kidney in experimental ovine trypanosomosis

2009 ◽  
Vol 76 (4) ◽  
Author(s):  
S.O. Omotainse ◽  
V.O. Anosa
Keyword(s):  
Lymph Nodes ◽  
Chronic Condition ◽  
Trypanosoma Congolense ◽  
Pathological Changes ◽  
Trypanosome Species ◽  
Chronic Infections ◽  
Phagocytic Cells ◽  
Liver And Kidney ◽  
Immunological Reactions ◽  
Spleen And Lymph Nodes

The infection of Yankassa rams with three important trypanosome species affecting livestock, namely, Trypanosoma congolense, T. vivax and T. brucei produced both acute and chronic fatal conditions. Chronic infections were induced in the three infections by the application of subcurative doses of diaminazene aceturate (Berenil®). Pathological changes in the infected animals included splenomegaly and hepatomegaly which were more pronounced in acute than in chronic T. congolense infection. However, these changes were more severe in chronic than in acute T. vivax infection. While splenomegaly was more pronounced in chronic T. brucei infection than in acute, hepatomegaly and lymphadenopathy were more severe in acute than in the chronic condition. The increases in size of the spleen, lymph nodes and liver were associated with congestion, increases in cell density related to increased immunological reactions in the spleen and lymph nodes as well as increase in numbers, size and activity of the phagocytic cells in these organs.

The route of re-circulation of lymphocytes in the rat

1964 ◽  
Vol 159 (975) ◽  
pp. 257-282 ◽  
Keyword(s):  
Lymph Nodes ◽  
Thoracic Duct ◽  
Large Scale ◽  
Plasma Cells ◽  
Tritiated Thymidine ◽  
Autoradiographic Study ◽  
Total Output ◽  
White Pulp ◽  
Radioactive Label

The experiments presented in this paper support the idea that the output of small lymphocytes from the thoracic duct of the rat (about 10 9 /day) is normally maintained by a large-scale re-circulation of cells from the blood to the lymph. It has been shown that the main channel from blood to lymph lies with in the lymph nodes and that small lymphocytes enter the nodes by crossing the walls of a specialized set of blood vessels, the post-capillary venules. In order to trace the fate of small lymphocytes, cells from the thoracic duct of rats were incubated for 1 h in vitro with tritiated adenosine. This labelled the RNA of about 65% of the small lymphocytes and more than 95% of the large lymphocytes; it also labelled the DNA of a proportion of the large lymphocytes. The mixture of small and large labelled lymphocytes was transfused into the blood of two groups of rats which belonged to the same highly inbred strain as the cell donors. At various times after the transfusions the thoracic ducts in one group of rats were cannulated to determine the proportion of labelled cells which could be recovered in the lymph; at corresponding times, the rats in the other group were killed and autoradiographs prepared from their tissues to determine the location of the labelled cells. The radioactive label in the RNA of small lymphocytes was stable enough to ensure that the labelled small lymphocytes which were recovered in the lymph several days after a transfusion were those which had originally been transfused into the blood. When the thoracic duct was cannulated 20 to 27 h after a transfusion, about 70% of the labelled small lymphocytes which had been transfused into the blood could be recovered from the thoracic duct over a 5-day period of lymph collection. During the first 36 to 48 h after cannulation, while the total output of small lymphocytes was falling rapidly, the proportion of labelled cells in the lymph remained approximately constant. The pool of the animal’s own cells with which the labelled cells had mixed contained between 1·5 and 2 × 10 9 small lymphocytes; this was identified as the re-circulating pool. An autoradiographic study showed that after their transfusion into the blood the labelled small lymphocytes ‘homed’ rapidly and in large numbers into the lymph nodes, the white pulp of the spleen and the Peyer’s patches of the intestine. The concentration of labelled cells in other tissues was trivial in comparison. Labelled small lymphocytes were seen penetrating the endothelium of the post-capillary venules in the lymph nodes within 15 min of the start of a transfusion; they were traced into the cortex of the nodes and finally into the medullary lymph sinuses. Labelled small lymphocytes did not migrate into the adult thymus but a few entered the thymus of newborn rats. It was concluded that the re-circulating pool of small lymphocytes was located in the lymphoid tissue, the thymus excepted, and that the rapid ‘homing’ of cells into the lymph nodes had its basis in the special affinity of small lymphocytes for the endothelium of the post-capillary venules. The interpretation of these experiments was not complicated by the presence of large, as well as of small lymphocytes in the suspensions of labelled cells which were transfused. Other experiments, in which the large lymphocytes alone were labelled with tritiated thymidine, showed that most of them migrated from the blood into the wall of the gut where they assumed the appearance of primitive plasma cells; very few divided to form small lymphocytes.

scholarly journals Detection of free cancer cells in peritoneal cavity in patients surgically treated for gastric adenocarcinoma

2006 ◽  
Vol 63 (4) ◽  
pp. 349-356 ◽  
Author(s):  
Zoran Kostic ◽  
Vladimir Cuk ◽  
Radojka Bokun ◽  
Dragan Ignjatovic ◽  
Slavica Usaj-Knezevic ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Cancer Cells ◽  
Gastric Adenocarcinoma ◽  
Peritoneal Cavity ◽  
Peritoneal Metastasis ◽  
Peritoneal Lavage ◽  
Abdominal Cavity ◽  
Cancer Invasion ◽  
Cytological Examination ◽  
Significant Difference

Bacground/Aim. Peritoneal metastasis is a leading cause of therapeutic failure after an operative treatment of patients with gastric adenocarcinoma. Free cancer cells might induce or indicate an early peritoneal seeding with a subsequent peritoneal metastasis. The aim of this study was to determine the frequency of the presence of free cancer cells in the peritoneal cavity in the patients surgically treated for gastric adenocarcinoma, and its relation to certain clinical, operative and pathohistological parameters. Methods. Inside a period from April 2000, and April 2004, the total of 100 patients underwent intraoperative peritoneal lavage for cytological examination. Immediately after the laparotomy, 200 ml physiologic saline, heated to 37 ?C, was introduced into the abdominal cavity, manually dispersed and collected from the region around the gastric tumor and the pouch of Douglas. The nucleated cell layer was smeared on four glass slides for every patient and dyed with May-Gr?nwald-Giemsa stain. The cytological findings were defined as positive or negative according to the presence of cancer cells. The frequency of positive cytological findings was compared to the location and the diameter of the cancer, pathohistological type of carcinoma, pathohistological stage of the disease, lymph node and the liver and/or peritoneal metastases and the type of surgical procedure. Results. Free cancer cells were found in 24 (24%) of the patients, while in 76 (76%) of them cytological findings were negative. A statistically highly significant difference (p ? 0.001) in the frequency of positive cytological finding was found between the groups of patients with and without cancer invasion of serosa, with cancer diameters > 5 cm and ? 5 cm, in the stage of disease I, II and III, IV, with macroscopically present and without metastases, with resection and D2 lymphadenectomy and palliative procedure. Free cancer cells were statistically more frequently (p ? 0.05) detected in the patients with lymph nodes metastases comparing to the patients without lymph nodes involvement. The results of the univariate analysis showed that the cancer diameter > 5 cm, tumor invasion of serosa, pathohistological stage of the disease III and IV and macroscopically visible metastases were the most important risk factors for the free cancer cells detection. Conclusion. Peritoneal lavage cytology was shown to be a useful tool for the detection of the group of patients with greatest risk of peritoneal dissemination. The frequency of positive cytological findings was highly associated with the diameter of the tumor and the cancer invasion of serosa. Cytological examination of peritoneal lavage fluid improved the accuracy of staging and selection of patients who might have benefit from neoadjuvant chemotherapy.

Role of fenestrated basement membrane in lymphatic absorption from peritoneal cavity

1959 ◽  
Vol 197 (3) ◽  
pp. 551-554 ◽  
Author(s):  
Lane Allen ◽  
Tim Weatherford
Keyword(s):  
Basement Membrane ◽  
Lymph Nodes ◽  
Peritoneal Cavity ◽  
Intraperitoneal Injection ◽  
Entire Population ◽  
Polystyrene Spheres ◽  
Lymphatic Absorption ◽  
Regional Lymph Nodes ◽  
Histological Picture

Polystyrene spheres with a range from chylomicron size up to 30 µ were injected into the peritoneal cavity of mouse, rat and cat, and were recovered from the regional lymph nodes. The largest recovered spheres in the mouse were 16.8 µ in diameter, in the rat and cat 24 µ. Inspection of the entire population of spheres recovered from lymph nodes 48 hours after intraperitoneal injection indicated that most of the fenestrations in the subperitoneal basement membrane are less than 5 µ in the mouse, and more than 5 µ in the cat. Fenestrations in the rat are intermediate between mouse and cat. The deductions as to fenestrations from inspection of the absorbed sphere populations is fairly well in accord with the histological picture in the mouse and cat. Many spheres reach the circulation and the larger ones are filtered out in the lungs, with resulting atelectasis.

scholarly journals STUDIES ON INFLAMMATION

1931 ◽  
Vol 53 (5) ◽  
pp. 647-660 ◽  
Author(s):  
Valy Menkin
Keyword(s):  
Lymph Nodes ◽  
Peritoneal Cavity ◽  
Normal Tissue ◽  
Capillary Permeability ◽  
Blood Stream ◽  
Regional Lymph Nodes ◽  
India Ink ◽  
Fibrin Network ◽  
Free Particles

India ink or graphite partides injected into an area of inflammation fail to disseminate to the tributary lymph nodes. When injected into a normal peritoneal cavity they rapidly appear in the retrosternal lymph nodes. When injected into an inflamed peritoneal cavity they are fixed in situ and fail to reach the regional lymph nodes. Graphite particles injected in the circulating blood stream enter an inflamed area both as free particles owing to increased capillary permeability and also as phagocyted material within leucocytes. Bacteria (B. prodigiosus) injected into inflamed tissue are fixed at the site of inflammation and fail to disseminate to the regional lymph nodes as readily as when injected into normal tissue. Bacteria (B. prodigiosus) injected at the periphery of an inflamed area do not readily penetrate into the site of inflammation. The experiments furnish evidence, in addition to that already provided, that fixation of foreign substances by the inflammatory reaction is primarily due to mechanical obstruction caused by a fibrin network and by thrombosed lymphatics at the site of inflammation. Bacteria (B. prodigiosus and B. pyocyaneus) injected intravenously rapidly enter an inflamed area. It is suggested that localization of bacteria in a locus minoris resistentiae may be explained as the result of increased capillary permeability with subsequent accumulation and fixation of bacteria from the blood stream at the point of injury.

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