scholarly journals Comparative histopathology of the lymph nodes, spleen, liver and kidney in experimental ovine trypanosomosis

2009 ◽  
Vol 76 (4) ◽  
Author(s):  
S.O. Omotainse ◽  
V.O. Anosa
Keyword(s):  
Lymph Nodes ◽  
Chronic Condition ◽  
Trypanosoma Congolense ◽  
Pathological Changes ◽  
Trypanosome Species ◽  
Chronic Infections ◽  
Phagocytic Cells ◽  
Liver And Kidney ◽  
Immunological Reactions ◽  
Spleen And Lymph Nodes

The infection of Yankassa rams with three important trypanosome species affecting livestock, namely, Trypanosoma congolense, T. vivax and T. brucei produced both acute and chronic fatal conditions. Chronic infections were induced in the three infections by the application of subcurative doses of diaminazene aceturate (Berenil®). Pathological changes in the infected animals included splenomegaly and hepatomegaly which were more pronounced in acute than in chronic T. congolense infection. However, these changes were more severe in chronic than in acute T. vivax infection. While splenomegaly was more pronounced in chronic T. brucei infection than in acute, hepatomegaly and lymphadenopathy were more severe in acute than in the chronic condition. The increases in size of the spleen, lymph nodes and liver were associated with congestion, increases in cell density related to increased immunological reactions in the spleen and lymph nodes as well as increase in numbers, size and activity of the phagocytic cells in these organs.

scholarly journals The Pathogenesis of Trypanosoma congolense Infection in Calves. I. Clinical Observations and Gross Pathological Changes

1978 ◽  
Vol 15 (5) ◽  
pp. 608-620 ◽  
Author(s):  
V. E. O. Valli ◽  
C. M. Forsberg ◽  
G. A. Robinson
Keyword(s):  
Lymph Nodes ◽  
Trypanosoma Congolense ◽  
Postmortem Examination ◽  
The Body ◽  
Red Cells ◽  
Pathological Changes ◽  
Intermittent Fever ◽  
Hair Coat ◽  
Clinical Observations ◽  
Holstein Calves

Holstein calves infected with Trypanosoma congolense TREU 112 had intermittent fever, debility and a poor hair coat. At postmortem examination the most significant findings were enlargement of the liver, kidneys and spleen and increased volume of hematopoietic marrow. A group of 13 calves infected for 18 weeks had marked enlargement of lymph nodes throughout the body and hemal nodes were prominent in the sublumbar area caudal to the kidneys. Another group infected for 7 weeks had no increase in the weight of lymph nodes. Studies with 51Cr labelled autologous red cells given shortly before postmortem examination showed that the liver was the most active organ with respect to sequestration of labelled red cells.

Pathological changes induced in avian liver and kidney after intramuscular injection of the crude venom and phospholipase A2 fraction of Bothrops insularis

Toxicon ◽  
1996 ◽  
Vol 34 (1) ◽  
pp. 17
Author(s):  
A.C.F. D'Abreu ◽  
C.C.L. Paronetto ◽  
J.C. Cogo ◽  
L. Rodrigues-Simioni ◽  
M.A. Cruz-Höfling
Keyword(s):  
Phospholipase A2 ◽  
Intramuscular Injection ◽  
Pathological Changes ◽  
Crude Venom ◽  
Liver And Kidney

scholarly journals Soufeng sanjie formula alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Di Hua ◽  
Jie Yang ◽  
Qinghai Meng ◽  
Yuanyuan Ling ◽  
Qin Wei ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Lymph Nodes ◽  
Inflammatory Cytokines ◽  
Th17 Cell ◽  
Collagen Induced Arthritis ◽  
Expression Levels ◽  
Th17 Cell Differentiation ◽  
Spleen And Lymph Nodes ◽  
Seed Coats

Abstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease. Soufeng sanjie formula (SF), which is composed of scolopendra (dried body of Scolopendra subspinipes mutilans L. Koch), scorpion (dried body of Buthus martensii Karsch), astragali radix (dried root of Astragalus membranaceus (Fisch.) Bge), and black soybean seed coats (seed coats of Glycine max (L.) Merr), is a traditional Chinese prescription for treating RA. However, the mechanism of SF in treating RA remains unclear. This study was aim to investigate the anti-arthritic effects of SF in a collagen-induced arthritis (CIA) mouse model and explore the mechanism by which SF alleviates arthritis in CIA mice. Methods For in vivo studies, female DBA/1J mice were used to establish the CIA model, and either SF (183 or 550 mg/kg/day) or methotrexate (MTX, 920 mg/kg, twice/week) was orally administered to the mice from the day of arthritis onset. After administration for 30 days, degree of ankle joint destruction and serum levels of IgG and inflammatory cytokines were determined. The balance of Th17/Treg cells in the spleen and lymph nodes was analyzed using flow cytometry. Moreover, the expression levels of retinoic acid receptor-related orphan nuclear receptor (ROR) γt and phosphorylated STAT3 (pSTAT3, Tyr705) in the spleen were detected by immunohistochemistry. Furthermore, the effect of SF on Th17 cells differentiation in vitro was investigated in CD4+ T cells under Th17 polarization conditions. Results SF decreased the arthritis score, ameliorated paw swelling, and reduced cartilage loss in the joint of CIA mice. In addition, SF decreased the levels of bovine collagen-specific IgG in sera of CIA mice. SF decreased the levels of inflammatory cytokines (TNF-α, IL-6, and IL-17A) and increased the level of IL-10 both in the sera and the joint of CIA mice. Moreover, SF treatment rebalanced the Th17/Treg ratio in the spleen and lymph nodes of CIA mice. SF also reduced the expression levels of ROR γt and pSTAT3 (Tyr705) in the spleen of CIA mice. In vitro, SF treatment reduced Th17 cell generation and IL-17A production and inhibited the expression of RORγt, IRF4, IL-17A, and pSTAT3 (Tyr705) under Th17 polarization conditions. Conclusions Our results suggest that SF exhibits anti-arthritic effects and restores Th17/Treg homeostasis in CIA mice by inhibiting Th17 cell differentiation.

scholarly journals EXTH-14. A NOVEL LONG-ACTING INTERLEUKIN-7 AGONIST, NT-I7, INCREASES CYTOTOXIC CD8 CELLS AND ENHANCES SURVIVAL IN MOUSE GLIOMA MODELS

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii89-ii89
Author(s):  
Subhajit Ghosh ◽  
Ran Yan ◽  
Sukrutha Thotala ◽  
Arijita Jash ◽  
Anita Mahadevan ◽  
...  
Keyword(s):  
T Cells ◽  
Lymph Nodes ◽  
Cd8 T Cells ◽  
Peripheral Blood ◽  
Cervical Lymph Nodes ◽  
Cd8 Cells ◽  
Interleukin 7 ◽  
Long Acting ◽  
Spleen And Lymph Nodes ◽  
Mouse Glioma

Abstract BACKGROUND Patients with glioblastoma (GBM) are treated with radiation (RT) and temozolomide (TMZ). These treatments can cause prolonged severe lymphopenia, which is associated with shorter survival. NT-I7 (efineptakin alfa) is a long-acting recombinant human IL-7 that supports the proliferation and survival CD4+ and CD8+ cells in both human and mice. We tested whether NT-I7 would protect T cells from treatment-induced lymphopenia and improve survival. METHODS C57BL/6 mice bearing intracranial tumors (GL261 or CT2A) were treated with RT (1.8 Gy/day x 5 days), TMZ (33 mg/kg/day x 5 days) and/or NT-17 (10 mg/kg on the final day of RT completion). We followed for survival and profiled CD3, CD8, CD4, FOXP3 in peripheral blood over time. In parallel, we assessed cervical lymph nodes, bone marrow, thymus, spleen, and the tumor 6 days after NT-I7 treatment. RESULTS Median survival in mice treated with NT-I7 combined with RT was significantly better than RT alone (GL261: 40d vs 34d, p< 0.0021; CT2A: 90d vs 40d, p< 0.0499) or NT-I7 alone (GL261: 40d vs 24d, p< 0.008; CT2A: 90d vs 32d, p< 0.0154). NT-17 with RT was just as effective as NT-I7 combined with RT and TMZ in both GL261 (40d vs 47d) and CT2A (90d vs 90d). NT-I7 treatment significantly increased the amount of CD8+ cells in the peripheral blood and tumor. NT- I7 rescued CD8+ T cells from RT induced lymphopenia in peripheral blood, spleen, and lymph nodes. NT-I7 alone or NT-I7 in combination with RT increased the CD8+ T cells in peripheral blood and tumor while reducing the FOXP3+ T-reg cells in the tumor microenvironment. CONCLUSIONS NT-I7 protects T-cells from RT induced lymphopenia, improves cytotoxic CD8+ T lymphocytes systemically and in the tumor, and improves survival. Presently, a phase I/II trial to evaluate NT-I7 in patients with high-grade gliomas is ongoing (NCT03687957).

An Unusual Case Involving the Spleen and Lymph Nodes

1989 ◽  
Vol 39 (3) ◽  
pp. 212-215
Author(s):  
Hitoshi Kubosawa ◽  
Akio Konno ◽  
Teisuke Komatsu ◽  
Hideo Ishige ◽  
Yoichiro Kondo
Keyword(s):  
Lymph Nodes ◽  
Unusual Case ◽  
Spleen And Lymph Nodes

scholarly journals A Ca2+-activated NADPH Oxidase in Testis, Spleen, and Lymph Nodes

2001 ◽  
Vol 276 (40) ◽  
pp. 37594-37601 ◽  
Author(s):  
Botond Bánfi ◽  
Gergely Molnár ◽  
Andres Maturana ◽  
Klaus Steger ◽  
Balázs Hegedûs ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Lymph Nodes ◽  
Spleen And Lymph Nodes
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