Unusual macromorphology of the ductuli efferentes and epididymis of the koala (Phascolarctos cinereus)

2000 ◽  
Vol 48 (6) ◽  
pp. 681
Author(s):  
Rachel J. Gibson ◽  
Chris M. Leigh ◽  
William G. Breed
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Secretory Activity ◽  
The Other ◽  
Ciliated Cells ◽  
Phascolarctos Cinereus ◽  
Ductuli Efferentes ◽  
Sds Page ◽  
Marsupial Species ◽  
Electron Lucent

The macromorphology of the ductuli efferentes and epididymis of the koala (Phascolarctos cinereus) was investigated and found to differ from that of other marsupial species that have been described as it comprised four macroscopically distinct lobes. Light and electron microscopy of epithelium of the duct within these lobes showed that there were principal and ciliated cells lining the duct of the first lobe, indicating it to be composed of ductuli efferentes. In the other three lobes, the epithelium contained principal, basal, electron-lucent, and mitochondria-rich cells, showing that these three lobes included the epithelium of the epididymis. The height of this epithelium gradually increased along the duct (contrary to the situation in most other species that have been studied, in which a decrease occurs). Preliminary 1D-SDS PAGE observations of flushes from the caput and cauda epididymides suggested that epididymal proteins were secreted along much of the length of the duct; the greater height of the cauda epithelium may relate to the greater protein synthetic and secretory activity in this region.

Mucolipidosis Type III α/β: The First Characterization of This Rare Disease by Autopsy

2011 ◽  
Vol 135 (4) ◽  
pp. 503-510
Author(s):  
Darcy A Kerr ◽  
Vincent A Memoli ◽  
Sara S Cathey ◽  
Brent T Harris
Keyword(s):  
Electron Microscopy ◽  
Heart Valves ◽  
Light And Electron Microscopy ◽  
Lysosomal Storage ◽  
Cytoplasmic Granules ◽  
Type Iii ◽  
Mucolipidosis Type ◽  
Electron Lucent ◽  
And Cartilage

Abstract We report findings from an autopsy of a 45-year-old woman with the rare lysosomal storage disease mucolipidosis type III α/β. Her disease manifested most notably as multiple bone and cartilage problems with tracheal and bronchial malacia. Principal autopsy findings included gross abnormalities in bone and cartilage with corresponding microscopic cytoplasmic lysosomal granules. These cytoplasmic granules were also seen in histologic preparations of the brain, myocardium, heart valves, and fibroblasts of the liver and skin by light and electron microscopy. By electron microscopy there were scattered, diffuse vesicular cytoplasmic granules in neurons and glia and an increase in lysosomal structures with fine electron lucent granularity in the above tissue types. Our findings help elaborate current understanding of this disease and differentiate it from the mucopolysaccharidoses and related disorders. To our knowledge, this is the first report to document pathologic findings in a patient with mucolipidosis type III α/β by autopsy.

Fine Structure of the Eye of a Nudibranch Mollusc, Hermissenda Crassicornis

1967 ◽  
Vol 2 (3) ◽  
pp. 349-358
Author(s):  
R. M. EAKIN ◽  
JANE A. WESTFALL ◽  
M. J. DENNIS
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Optic Nerve ◽  
Light And Electron Microscopy ◽  
The Other ◽  
Sensory Cells ◽  
Nerve Fibres ◽  
Supporting Cells ◽  
Small Bodies ◽  
Receptor Cells

The eye of a nudibranch, Hermissenda crassicornis, was studied by light and electron microscopy. Three kinds of cells were observed: large sensory cells, each bearing at one end an array of microvilli (rhabdomere) and at the other end an axon which leaves the eye by the optic nerve; large pigmented supporting cells; and small epithelial cells, mostly corneal. There are five sensory cells, and the same number of nerve fibres in the optic nerve. The receptor cells contain an abundance of small vesicles, 600-800 Å in diameter. The lens is a spheroidal mass of osmiophilic, finely granular material. A basal lamina and a capsule of connective tissue enclose the eye. In some animals the eye is ‘infected’ with very small bodies, 4-5 µ in diameter, thought to be symbionts.

Ultrastructure of eastern cottonwood clones susceptible or resistant to leaf rust

1987 ◽  
Vol 65 (8) ◽  
pp. 1586-1598 ◽  
Author(s):  
L. Shain ◽  
U. Järlfors
Keyword(s):  
Electron Microscopy ◽  
Leaf Rust ◽  
Light And Electron Microscopy ◽  
Infection Process ◽  
The Other ◽  
Host Cells ◽  
Eastern Cottonwood ◽  
Melampsora Medusae ◽  
Wall Appositions ◽  
Infected Cells

The infection process in four clones of eastern cottonwood susceptible or resistant to leaf rust caused by Melampsora medusae was studied by light and electron microscopy. Infection was initiated by stomatal rather than direct entry. Typical dikaryotic haustoria were observed in all clones within 1 day of inoculation. Some healthy-appearing haustoria were observed in susceptible clones throughout the duration of the study, which was terminated during the initiation of uredial production. Incompatibility was expressed differently in the two resistant clones. In clone St 75, most haustoria and invaded host cells that were observed appeared necrotic within 2 days of inoculation. Cell wall appositions appeared during this time in cells adjoining necrotic host cells. Some infected cells disintegrated within 4 days of inoculation. Affected host cells of clone St 92, on the other hand, plasmolyzed during the first 2 to 3 days after inoculation. Necrotic host cells were not observed in this clone until the 4th day after inoculation. Hyphal ramification and host plasmolysis were extensive at 6 days after inoculation.

Asci of the Pezizales. I. The apical apparatus of iodine-positive species

1978 ◽  
Vol 56 (16) ◽  
pp. 1860-1875 ◽  
Author(s):  
Don A. Samuelson
Keyword(s):  
Electron Microscopy ◽  
Positive Reaction ◽  
Light And Electron Microscopy ◽  
The Other ◽  
Reaction Site ◽  
Lower Region

Morphological, developmental, and cytochemical studies on the apical apparatuses of five species, i.e., Peziza succosa, Ascobolus crenulatus, Saccobolus depauperatus, Thecotheus pelletieri, and Iodophanus granulipolaris, were performed with light and electron microscopy. Asci of all species, except A. crenulatus, stain blue in Melzer's reagent. The site of the iodine-positive reaction is believed to be an exogenous mucilaginous coat in P. succosa, S. depauperatus, and T. pelletieri. In I. granulipolaris, the reaction site appears to be the ascal wall. The presence of an annular indentation was found in the ascal tips of all species except I. granulipolaris. A line of dehiscence was found in the lower region of the annular indentation in T. pelletieri and S. depauperatus. The development of the apical apparatuses of all species occurs during and after late ascosporogenesis. The apical apparatus of I. granulipolaris diverged significantly in morphology and cytochemistry from the other species.

Somatic mitosis in Erysiphe graminis hordei

1972 ◽  
Vol 18 (12) ◽  
pp. 1915-1922 ◽  
Author(s):  
W. E. McKeen
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Nuclear Membrane ◽  
Osmium Tetroxide ◽  
Central Body ◽  
Nuclear Division ◽  
Light And Electron Microscopy ◽  
Erysiphe Graminis ◽  
The Other ◽  
Erysiphe Graminis Hordei

Somatic nuclear division in Erysiphe graminis hordei was studied by light and electron microscopy after various fixation and staining procedures. Electron microscopy studies of alcohol – acetic acid fixed material aided in providing an understanding of nuclear division and showing the gross alterations which occurred. Light microscopy indicated that a central body was always present at a specific site on the nuclear membrane in the interphase nucleus and was connected to chromatic spherical bodies. Microtubules were preserved when a short glutaraldehyde – osmium tetroxide fixation was used. Some microtubules extend from plaque to plaque while others terminate in kinetochores. A microtubular spindle, oblique to the nuclear and mildew-cells axes formed within the nuclear membrane. Typical prophases, metaphases, anaphases, and telophases were observed. Then one set of daughter chromatids bypassed the nucleolus which persisted intranuclearly until the daughter nuclei reached their destination, and the other set of daughter chromatids moved to midpoint in the other daughter cell. A narrow corridor, which connected daughter nuclei for some time, was filled mainly with microtubules and probably was the filament which was observed in the nucleus by light microscopy during nuclear division. At least six chromosomes were present in each nucleus.

Cyclic alterations in rhesus monkey endometrium by scanning electron microscopy

1995 ◽  
Vol 7 (5) ◽  
pp. 1199 ◽  
Author(s):  
D Bhartiya ◽  
VK Bajpai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Menstrual Cycle ◽  
Morphological Characteristics ◽  
Secretory Activity ◽  
Cell Number ◽  
Ciliated Cells ◽  
Menstrual Phase ◽  
Negative Effect ◽  
Scanning Electron

The present study was undertaken to correlate the surface topography of endometrium with altered concentrations of circulating steroids during different phases of the artificially-induced menstrual cycle. Scanning electron microscopy demonstrated that oestradiol during the oestrogenic phase induces an increase in the number of ciliated cells and the size of microvilli; by contrast, progesterone, in addition to inducing secretory activity in non-ciliated cells, had a negative effect on oestradiol-dependent morphological characteristics of ciliated cells, causing a reduction in cell number and deciliation, and inducing drooping of cilia during the progestogenic phase of the artificially-induced menstrual cycle. In addition, surface and glandular epithelial cells both actively participate in restoration of the endometrial surface during the menstrual phase, an event which parallels endometrial shedding.

scholarly journals The istant changes of the salivary glands to stress

2010 ◽  
Vol 9 (1) ◽  
pp. 31-35
Author(s):  
A. V. Gerasimov ◽  
S. V. Logvinov ◽  
V. P. Kostyuchenko
Keyword(s):  
Electron Microscopy ◽  
Salivary Glands ◽  
Guinea Pigs ◽  
Light And Electron Microscopy ◽  
Secretory Activity ◽  
Duct Cell ◽  
Cell Stress ◽  
Microwave Exposure ◽  
Submandibular Salivary Glands ◽  
Granular Ducts

Night lighting and microwave exposure have been influence on structures of stress realization. The endocrine, fotoperiodical and adaptive functions of rodent submandibular salivary glands belonging to hormone produced duct cells. To evaluate their morphofunctional state at guinea-pigs and rats using the methods of light and electron microscopy there have been analyzed striated and granular ducts. It has been revealed that instant and twenty-four-hour strengthening of duct cell stress induced secretory activity was similar. It is concluded that salivary glands take part in circadian expectations to stress.

Effects of sulfur dioxide on pore populations of canine tracheal epithelium

1986 ◽  
Vol 60 (2) ◽  
pp. 416-426 ◽  
Author(s):  
S. F. Man ◽  
W. C. Hulbert ◽  
K. Mok ◽  
T. Ryan ◽  
A. B. Thomson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Barrier Properties ◽  
The Other ◽  
Compressed Air ◽  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
High Concentrations ◽  
Scanning Electron ◽  
Nonelectrolyte Permeability

The bioelectric and barrier properties of the tracheal epithelium in nose-breathing dogs and in dogs that had been exposed for 75 min to compressed air or to two high concentrations of SO2 were measured and compared. We also studied tissues that had been treated with chloroform. Based on a model of restrictive diffusion we demonstrated heteropores (6 and 250 A) in the control tissues. Bioelectric changes due to 100-ppm SO2 were minimal. After exposure to 500 ppm SO2, adverse changes in the bioelectric properties were focal; they were marked in 8 out of 12 animals but were less striking in the other 4. Nonelectrolyte permeability increased with an increase in SO2 concentrations. Small pores were still present in the tissues severely affected by SO2 but they were absent in chloroform-treated tissues. Scanning electron microscopy of tissues from animals exposed to 500 ppm SO2 showed that in the same dog tissue appearance varied from normal to one of repair (normal bioelectric properties) or one of marked exfoliation of ciliated cells (abnormal bioelectric measurements).

Ultrastructure of Mucous Blanket in Otitis Media with Effusion

1988 ◽  
Vol 97 (3) ◽  
pp. 313-317 ◽  
Author(s):  
Masashi Inagaki ◽  
Yasuo Sakakura ◽  
Yuichi Majima ◽  
Takeshi Shimizu ◽  
Kotaro Ukai
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Ciliated Cells ◽  
Ciliary Movement ◽  
Mucous Layer ◽  
Transmission Electron ◽  
Electron Lucent

We used transmission electron microscopy to study the mucous blanket of the promontory from children with otitis media with effusion. The vast majority of the epithelial cells were secretory, and the rest were ciliated. The mucous blanket consisted of the electron-lucent periciliary fluid and the mucous layer. In the mucous layer, two layers were identified: An inner layer with migrating cells, and an outer layer with specks. Moreover, there was a lucent zone over the nonciliated surface that was as high as the microvilli. The thickness of the periciliary layer was predominantly as great as that of the ciliary tips, which just make contact with the mucous layer; however, the mucous layer occasionally penetrated into the periciliary space. These findings indicated that there is a mucociliary dysfunction in the middle ear caused by a decrease in the number of ciliated cells, and an abnormal interaction between cilia and mucus that would interfere with ciliary movement. Thus, such a system would fail to transport the mucous blanket.

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