scholarly journals 3. In vivo electroporation methodologies to study male reproductive gene expression

2003 ◽  
Vol 15 (9) ◽  
pp. 3
Author(s):  
J. L. Kirby ◽  
R. J. Lye ◽  
J. C. Labus ◽  
B. T. Hinton
Keyword(s):  
Gene Expression ◽  
In Vivo Electroporation

scholarly journals Evaluation of three different promoters driving gene expression in developing chicken embryo by using in vivo electroporation

2014 ◽  
Vol 13 (1) ◽  
pp. 1270-1277 ◽  
Author(s):  
C.Q. Yang ◽  
X.Y. Li ◽  
Q. Li ◽  
S.L. Fu ◽  
H. Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chicken Embryo ◽  
In Vivo Electroporation

In vivo electroporation and ubiquitin promoter – a protocol for sustained gene expression in the lung

2006 ◽  
Vol 8 (7) ◽  
pp. 910-918 ◽  
Author(s):  
Amiq Gazdhar ◽  
Murat Bilici ◽  
Jaroslaw Pierog ◽  
Erick L Ayuni ◽  
Mathias Gugger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ubiquitin Promoter ◽  
In Vivo Electroporation

Foreign gene expression in an organotypic culture of cortical anlage after in vivo electroporation

Neuroreport ◽  
1999 ◽  
Vol 10 (11) ◽  
pp. 2319-2323 ◽  
Author(s):  
Nobuhiko Miyasaka ◽  
Yasuyoshi Arimatsu ◽  
Keiko Takiguchi-Hayashi
Keyword(s):  
Gene Expression ◽  
Organotypic Culture ◽  
Foreign Gene ◽  
In Vivo Electroporation ◽  
Foreign Gene Expression

scholarly journals Analysis of mineral apposition rates during alveolar bone regeneration over three weeks following transfer of BMP-2/7 gene via in vivo electroporation

2018 ◽  
Author(s):  
Mariko Kawai ◽  
Yohei Kataoka ◽  
Junya Sonobe ◽  
Hiromitsu Yamamoto ◽  
Hiroki Maruyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Bone Regeneration ◽  
Expression Vector ◽  
Alveolar Bone ◽  
Plasmid Vector ◽  
Periodontal Tissue ◽  
In Vivo Electroporation ◽  
Regeneration Potential

Alveolar bone is not spontaneously regenerated following trauma or periodontitis. We previously proposed an animal model for new alveolar bone regeneration therapy based on the non-viral BMP-2/7 gene expression vector and in vivo electroporation, which induced the formation of new alveolar bone over the course of a week. Here, we analysed alveolar bone during a period of three weeks following gene transfer to periodontal tissue. Non-viral plasmid vector pCAGGS-BMP-2/7 or pCAGGS control was injected into palatal periodontal tissue of the first molar of the rat maxilla and immediately electroporated with 32 pulses of 50 V for 50 msec. Over the following three weeks, rats were double bone-stained by calcein and tetracycline every three days and mineral apposition rates (MAR) were measured. Double bone-staining revealed that MAR of alveolar bone was as similar level three days before BMP-2/7 gene transfer as three days after gene transfer. However, from 3 to 6 days, 6 to 9 days, 9 to 12 days, 12 to 15 days, 15 to 18 days, and 18 to 20 days after, MARs were significantly higher than prior to gene transfer. Our proposed gene therapy for alveolar bone regeneration combining non-viral BMP-2/7 gene expression vector and in vivo electroporation could increase alveolar bone regeneration potential in the targeted area for up to three weeks.

Delivery of Plasmid DNA Through Intratumoral Infusion and Electroporation

2000 ◽  
Author(s):  
Fan Yuan ◽  
David Zaharoff ◽  
Xiao-Yu Zhang ◽  
Frank Lohr ◽  
Mark W. Dewhirst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Solid Tumors ◽  
Plasmid Dna ◽  
Ex Vivo ◽  
Electric Pulse ◽  
Interleukin 12 ◽  
Growth Delay ◽  
In Vivo Electroporation ◽  
Electric Pulses

Abstract We investigated DNA transport in the interstitial space and across cell membrane facilitated by intratumoral infusion and in vivo electroporation, respectively. In the study, a rat fibrosarcoma was perfused ex vivo, and apparent hydraulic conductivity (Kapp) was quantified under different perfusion conditions. In addition, three plasmid DNA vectors were infused into solid tumors. Immediately after infusion, tumors were treated with or without electric pulses. Gene expression and tumor growth delay were determined at different time points after electroporation. We found that Kapp was very sensitive to the perfusion pressure, presumably due to perfusion-induced tissue deformation. Treatment of tumors with electric pulse facilitated gene expression in vivo. The growth of tumors treated with plasmid DNA encoding interleukin 12 (IL-12) and electric pulses was slower than those treated with IL-12 or electric pulses alone. These data suggest that gene delivery in solid tumors could be improved significantly through combination of intratumoral infusion and in vivo electroporation.

Delivery of Plasmid DNA Through Intratumoral Infusion and Electroporation

2000 ◽  
Author(s):  
Fan Yuan ◽  
David Zaharoff ◽  
Xiao-Yu Zhang ◽  
Frank Lohr ◽  
Mark W. Dewhirst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Solid Tumors ◽  
Plasmid Dna ◽  
Ex Vivo ◽  
Electric Pulse ◽  
Interleukin 12 ◽  
Growth Delay ◽  
In Vivo Electroporation ◽  
Electric Pulses

Abstract We investigated DNA transport in the interstitial space and across cell membrane facilitated by intratumoral infusion and in vivo electroporation, respectively. In the study, a rat fibrosarcoma was perfused ex vivo, and apparent hydraulic conductivity (Kapp) was quantified under different perfusion conditions. In addition, three plasmid DNA vectors were infused into solid tumors. Immediately after infusion, tumors were treated with or without electric pulses. Gene expression and tumor growth delay were determined at different time points after electroporation. We found that Kapp was very sensitive to the perfusion pressure, presumably due to perfusion-induced tissue deformation. Treatment of tumors with electric pulse facilitated gene expression in vivo. The growth of tumors treated with plasmid DNA encoding interleukin 12 (IL-12) and electric pulses was slower than those treated with IL-12 or electric pulses alone. These data suggest that gene delivery in solid tumors could be improved significantly through combination of intratumoral infusion and in vivo electroporation.

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