Gene Therapy for Bone Regeneration using Non-viral BMP Gene Expression Vector and in vivo Electroporation

Alveolar bone is not spontaneously regenerated following trauma or periodontitis. We previously proposed an animal model for new alveolar bone regeneration therapy based on the non-viral BMP-2/7 gene expression vector and in vivo electroporation, which induced the formation of new alveolar bone over the course of a week. Here, we analysed alveolar bone during a period of three weeks following gene transfer to periodontal tissue. Non-viral plasmid vector pCAGGS-BMP-2/7 or pCAGGS control was injected into palatal periodontal tissue of the first molar of the rat maxilla and immediately electroporated with 32 pulses of 50 V for 50 msec. Over the following three weeks, rats were double bone-stained by calcein and tetracycline every three days and mineral apposition rates (MAR) were measured. Double bone-staining revealed that MAR of alveolar bone was as similar level three days before BMP-2/7 gene transfer as three days after gene transfer. However, from 3 to 6 days, 6 to 9 days, 9 to 12 days, 12 to 15 days, 15 to 18 days, and 18 to 20 days after, MARs were significantly higher than prior to gene transfer. Our proposed gene therapy for alveolar bone regeneration combining non-viral BMP-2/7 gene expression vector and in vivo electroporation could increase alveolar bone regeneration potential in the targeted area for up to three weeks.

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Evaluation of three different promoters driving gene expression in developing chicken embryo by using in vivo electroporation

Genetics and Molecular Research ◽

10.4238/2014.february.27.12 ◽

2014 ◽

Vol 13 (1) ◽

pp. 1270-1277 ◽

Cited By ~ 7

Author(s):

C.Q. Yang ◽

X.Y. Li ◽

Q. Li ◽

S.L. Fu ◽

H. Li ◽

...

Keyword(s):

Gene Expression ◽

Chicken Embryo ◽

In Vivo Electroporation

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Cancer gene therapy using in vivo electroporation of Flt3-ligand

International Journal of Oncology ◽

10.3892/ijo.27.2.457 ◽

2005 ◽

Cited By ~ 1

Author(s):

Kazuya Shimao ◽

Takuya Takayama ◽

Katsuhisa Enomoto ◽

Tetsuya Saito ◽

Shigenori Nagai ◽

...

Keyword(s):

Gene Therapy ◽

Cancer Gene Therapy ◽

Cancer Gene ◽

Flt3 Ligand ◽

In Vivo Electroporation

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In vivo “LIVER OFF” “TUMOR ON” gene expression toward the gene therapy of colon cancer liver metastasis using adenovirus vector with cyclooxygenase-2 promoter

Gastroenterology ◽

10.1016/s0016-5085(00)82781-x ◽

2000 ◽

Vol 118 (4) ◽

pp. A176

Author(s):

Masato Yamamoto ◽

Ramon Alemany ◽

Yasuo Adachi ◽

Kaori Suzuki ◽

David T. Curiel

Keyword(s):

Gene Expression ◽

Gene Therapy ◽

Colon Cancer ◽

Liver Metastasis ◽

Adenovirus Vector ◽

Cyclooxygenase 2 ◽

Colon Cancer Liver Metastasis

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Cytokine Gene Therapy for Myocarditis by In Vivo Electroporation

Human Gene Therapy ◽

10.1089/104303401750270940 ◽

2001 ◽

Vol 12 (10) ◽

pp. 1289-1297 ◽

Cited By ~ 45

Author(s):

Atsushi Nakano ◽

Akira Matsumori ◽

Shunsuke Kawamoto ◽

Hideaki Tahara ◽

Eiji Yamato ◽

...

Keyword(s):

Gene Therapy ◽

Cytokine Gene ◽

In Vivo Electroporation ◽

Cytokine Gene Therapy

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Gene Therapy Platform for Bone Regeneration Using an Exogenously Regulated, AAV-2-Based Gene Expression System

Molecular Therapy ◽

10.1016/j.ymthe.2003.12.009 ◽

2004 ◽

Vol 9 (4) ◽

pp. 587-595 ◽

Cited By ~ 97

Author(s):

Yossi Gafni ◽

Gadi Pelled ◽

Yoram Zilberman ◽

Gadi Turgeman ◽

Florence Apparailly ◽

...

Keyword(s):

Gene Expression ◽

Gene Therapy ◽

Bone Regeneration ◽

Expression System ◽

Gene Expression System

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The Antisense RNA Approach: a New Application forIn VivoInvestigation of the Stress Response of Oenococcus oeni, a Wine-Associated Lactic Acid Bacterium

Applied and Environmental Microbiology ◽

10.1128/aem.02495-15 ◽

2015 ◽

Vol 82 (1) ◽

pp. 18-26 ◽

Cited By ~ 11

Author(s):

Maud Darsonval ◽

Tarek Msadek ◽

Hervé Alexandre ◽

Cosette Grandvalet

Keyword(s):

Gene Expression ◽

Lactic Acid ◽

Stress Response ◽

Lactic Acid Bacterium ◽

Antisense Rna ◽

Expression Vector ◽

Stress Proteins ◽

Oenococcus Oeni ◽

Content Type

ABSTRACTOenococcus oeniis a wine-associated lactic acid bacterium mostly responsible for malolactic fermentation in wine. In wine,O. oenigrows in an environment hostile to bacterial growth (low pH, low temperature, and ethanol) that induces stress response mechanisms. To survive,O. oeniis known to set up transitional stress response mechanisms through the synthesis of heat stress proteins (HSPs) encoded by thehspgenes, notably a unique small HSP named Lo18. Despite the availability of the genome sequence, characterization ofO. oenigenes is limited, and little is known about thein vivorole of Lo18. Due to the lack of genetic tools forO. oeni, an efficient expression vector inO. oeniis still lacking, and deletion or inactivation of thehsp18gene is not presently practicable. As an alternative approach, with the goal of understanding the biological function of theO. oenihsp18genein vivo, we have developed an expression vector to produce antisense RNA targeting ofhsp18mRNA. Recombinant strains were exposed to multiple stresses inducinghsp18gene expression: heat shock and acid shock. We showed that antisense attenuation ofhsp18affectsO. oenisurvival under stress conditions. These results confirm the involvement of Lo18 in heat and acid tolerance ofO. oeni. Results of anisotropy experiments also confirm a membrane-protective role for Lo18, as previous observations had already suggested. This study describes a new, efficient tool to demonstrate the use of antisense technology for modulating gene expression inO. oeni.

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