164 Effect of dissolving solution on embryo recovery results of superovulation with FSH single subcutaneous injection

2022 ◽  
Vol 34 (2) ◽  
pp. 320
Author(s):  
T. Maeda ◽  
A. Katae ◽  
T. Terashima ◽  
A. Yokota ◽  
M. Sugawara ◽  
...  
Keyword(s):  
Subcutaneous Injection ◽  
Single Subcutaneous Injection ◽  
Embryo Recovery

292 EFFECT OF A SINGLE INJECTION OF FSH DISSOLVED IN POLYETHYLENE GLYCOL ON SUPEROVULATION RESPONSE IN KOREAN NATIVE COWS

2009 ◽  
Vol 21 (1) ◽  
pp. 243
Author(s):  
S. H. Choi ◽  
K. H. Cho ◽  
Y. S. Park
Keyword(s):  
Polyethylene Glycol ◽  
Pregnancy Rate ◽  
Subcutaneous Injection ◽  
Linear Models ◽  
Single Injection ◽  
Simple Procedure ◽  
Single Subcutaneous Injection ◽  
Embryo Recovery ◽  
Surgical Methods ◽  
Group 2

Superovulation in bovine is usually achieved after several FSH injection in a time-consuming protocol, and the half-life of FSH is very short. This study was carried out to assess the efficiency of embryo production after induction of superovulation with a single injection of FSH (Folltropin-V, Vetrepharm, Canada) dissolved in polyethylene glycol (PEG, 8000 MW, Sigma-Aldrich Corp., St. Louis, MO, USA) in Korean Native cows. Eighty-eight cows were randomly assigned to one of four groups. In Group 1 (control), cows were intramuscularly treated with twice-daily administration of 50 mg FSH for 4 days. In Group 2 and 3, cows received a single subcutaneous injection of 400 mg and 200 mg FSH dissolved in 30% PEG, respectively. In Group 4, cows received a single subcutaneous injection of 200 mg FSH dissolved in 30% PEG given seven days after CIDR insertion. On days 10 to 12 after observed estrus, superovulation was started. Luteolysis was induced by administration of 25 mg of PGF2α (Pharmacia, Puurs, Belgium) IM 2 days after the start of the FSH injection. Donors were timed-inseminated with frozen–thawed semen at 48 and 60 h after the administration of PGF2α. Embryos were collected by non-surgical methods on 7 days after the second AI. The numbers of corpus lutea (CL), total ova, transferable embryos, and progesterone concentration were analyzed by General Linear Models Procedure with the Statistical Analysis System (SAS; SAS Institute, Inc., Cary, NC, USA) and means were compared with the Duncan’s multiple range test. Data on the pregnancy rate was analyzed by chi-square test. P values <0.05 were considered to be significantly different. The number of CL in Group 2 was significantly (P < 0.05) higher compared to Groups 1, 3 and 4 (18.5 ± 7.5 v. 11.2 ± 5.1, 13.1 ± 5.1 and 13.9 ± 6.4, respectively). However, the number of total ova (7.9 ± 6.0 to 10.4 ± 7.2) and transferable embryos (3.7 ± 3.3 to 4.7 ± 3.9) did not differ among treatment groups. In addition, blood progesterone concentrations at embryo recovery did not differ among all groups (10.0 ± 3.5 ng mL–1 to 15.7 ± 3.2 ng mL–1). No difference was observed among groups in pregnancy rate after transferring the recovered embryos (36.0 to 50.0%). In conclusion, our results show that a single injection of FSH dissolved in polyethylene glycol is an efficient and simple procedure for inducing superovulation in Korean native cows.

Superovulatory response to single subcutaneous injection of Folltropin in Holstein and Sahiwal cows

1992 ◽  
Vol 37 (1) ◽  
pp. 260 ◽  
Author(s):  
A.K. Misra ◽  
S.A. Chaubal ◽  
G. Krishna Kishore ◽  
S. Rajeshwaran ◽  
B.V. Joshi ◽  
...  
Keyword(s):  
Subcutaneous Injection ◽  
Single Subcutaneous Injection ◽  
Sahiwal Cows

HORMONAL REQUIREMENTS FOR THE MAINTENANCE OF OESTRADIOL-INDUCED INHIBITION OF UTERINE SENSITIVITY IN THE OVARIECTOMIZED RAT

1970 ◽  
Vol 46 (3) ◽  
pp. 341-346 ◽  
Author(s):  
K. P. MEYERS
Keyword(s):  
Subcutaneous Injection ◽  
Ovariectomized Rats ◽  
Ovariectomized Rat ◽  
Single Subcutaneous Injection ◽  
The Third ◽  
Oestradiol Treatment ◽  
Progesterone Treatment ◽  
Endometrial Scratching

SUMMARY Ovariectomized rats treated with 2·5 or 5·0 mg. progesterone daily received a single subcutaneous injection of 0·2 μg. oestradiol on the third day of the progesterone treatment. The deciduomal response to trauma by endometrial scratching was used to determine the degree of uterine sensitivity at various times after oestradiol. Uterine sensitivity was partially and then completely lost 36 and 48 hr. after oestradiol administration. The inhibition of uterine sensitivity persisted until 9 and 11 days after oestradiol when the animals received 2·5 and 5·0 mg. progesterone daily. Uterine sensitivity was completely inhibited on day 11 with doses of oestradiol from 0·2 to 0·05 μg. Withdrawal of progesterone treatment for 48 or 72 hr., but not for 24 hr., after oestradiol treatment restored uterine sensitivity. These results show that the oestradiol-induced inhibition of uterine sensitivity in the progestational endometrium is maintained by continuous progesterone treatment and that the duration of this effect is dependent on the dose of progesterone given.

OBSERVATIONS ON CADMIUM DAMAGE AND REPAIR IN RAT TESTES AND THE EFFECTS ON THE PITUITARY GONADOTROPHS

1962 ◽  
Vol 24 (4) ◽  
pp. 453-NP ◽  
Author(s):  
M. ALLANSON ◽  
R. DEANESLY
Keyword(s):  
Subcutaneous Injection ◽  
Cadmium Chloride ◽  
Cytological Study ◽  
Interstitial Cells ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Long Term Effects ◽  
Single Subcutaneous Injection

SUMMARY Cadmium chloride, in a single subcutaneous injection, can destroy spermatogenic and interstitial cells in the rat testis (Pařízek, 1957) and produce changes in the pituitary. The interstitial tissue is restored by ingrowths from the tunica and full androgen secretion returns before there is any regeneration of germinal epithelium. A cytological study has been made of the peripheral and central pituitary gonadotrophs; the latter revert almost to normal as the interstitial tissue regenerates, whereas the former retain characteristic castration features, unless there is also regeneration of the germinal epithelium. This seems to indicate that in the normal testis there is a hormone contribution from the seminiferous tubules as well as from the interstitial cells. The long-term effects of cadmium on the testis depend on the dose. Early stages of tubule restoration have been studied, but after administration of 0·9 mg., actual proliferation of the germinal epithelium was rarely found—only in four out of twenty rats, 113 or 142 days after injection.

scholarly journals Dissemination and immunogenicity of live TRIC agent in baboons after parenteral injection: II. Experiments with a ‘slow-killing’ strain

1969 ◽  
Vol 67 (3) ◽  
pp. 449-455 ◽  
Author(s):  
L. H. Collier ◽  
Anne E. Mogg(Née Smith)
Keyword(s):  
Lymph Nodes ◽  
Subcutaneous Injection ◽  
Technical Assistance ◽  
Regional Lymph Nodes ◽  
Virulent Mutant ◽  
Single Subcutaneous Injection ◽  
Single Intravenous Injection ◽  
Peripheral Lymph ◽  
Homologous Strain ◽  
The Difference

SUMMARYAfter a single subcutaneous injection into baboons the MRC-4 strain of trachoma/inclusion conjunctivitis (TRIC) agent underwent limited multiplication at the injection site, but was then eliminated rapidly from the skin and regional lymph nodes. Forty-eight hours after a single intravenous injection, but not thereafter, it appeared in the peripheral lymph nodes and spleen. The single parenteral injections failed to immunize baboons against conjunctival challenge with the homologous strain. These findings contrasted with those previously reported for the more virulent mutant, MRC-4 f, which multiplied readily in the skin, lymph nodes and spleen, persisted in these tissues up to 3 weeks after injection, and conferred good immunity to conjunctival challenge with MRC-4. The difference in behaviour of MRC-4 and MRC-4 f might be accounted for, at least in part, by the use of a smaller inoculum of live MRC-4; but similar findings in guinea-pigs, reported elsewhere, suggest that the differences observed are real. In conjunction with previous work, the present study suggests that the immunogenicity of TRIC agent is closely related to the mass of antigen that can be administered to or propagated within the recipient.We are grateful to Mr D. Venters for his able technical assistance.

SENSITIZATION BY INSULIN TO THE DEXTRAN "ANAPHYLACTOID" REACTION

1957 ◽  
Vol 35 (1) ◽  
pp. 251-256 ◽  
Author(s):  
V. W. Adamkiewicz ◽  
Y. Langlois
Keyword(s):  
Small Dose ◽  
Subcutaneous Injection ◽  
Anaphylactoid Reaction ◽  
Crystalline Insulin ◽  
Single Subcutaneous Injection ◽  
Small Doses

A single subcutaneous injection of crystalline insulin (20 units) sensitizes considerably the rats to the dextran "anaphylactoid" reaction. This is a type of acute serous inflammation. Insulin precipitates the reaction after a very small dose of dextran (0.05–0.01 ml., 6% solution) has been injected into a "shock organ". Without insulin, such small doses of dextran are quite ineffective. Insulin also precipitates the reaction when dextran (1.0 ml., 6% solution) is injected peripherally on the back. In this site and at this dose, it rarely produces the reaction in normal rats. The sensitization manifests itself despite a cortisone pretreatment.

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