145 Comparison of various buffalo sera collected during different phases of estrous cycle for in vitro maturation and culturing of Nili-Ravi buffalo oocytes

2022 ◽  
Vol 34 (2) ◽  
pp. 310
Author(s):  
M. Waseem ◽  
M. Irfan-ur-Rehman Khan ◽  
M. Usman Mehmood ◽  
A. Riaz ◽  
M. Akhtar
Keyword(s):  
Estrous Cycle ◽  
In Vitro Maturation

Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes

2010 ◽  
Vol 117 (1-2) ◽  
pp. 160-165 ◽  
Author(s):  
Mithat Evecen ◽  
Ümüt Cirit ◽  
Kamber Demir ◽  
Özen Banu Özdaş ◽  
Muzaffer Taş ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
In Vitro Maturation ◽  
Estrous Cycle Stage

scholarly journals Oocyte Competence for In Vitro Maturation Is Associated with Histone H1 Kinase Activity and Is Influenced by Estrous Cycle Stage in the Mare1

1998 ◽  
Vol 59 (2) ◽  
pp. 456-462 ◽  
Author(s):  
Ghylène Goudet ◽  
Jacqueline Bézard ◽  
François Belin ◽  
Guy Duchamp ◽  
Eric Palmer ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
In Vitro Maturation ◽  
Kinase Activity ◽  
Histone H1 ◽  
Oocyte Competence ◽  
Estrous Cycle Stage

Estrous cycle impacts microRNA content in extracellular vesicles that modulate bovine cumulus cell transcripts during in vitro maturation†

2019 ◽  
Vol 102 (2) ◽  
pp. 362-375 ◽  
Author(s):  
Ana Clara Faquineli Cavalcante Mendes de Ávila ◽  
Alessandra Bridi ◽  
Gabriella Mamede Andrade ◽  
Maite del Collado ◽  
Juliano Rodrigues Sangalli ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Extracellular Vesicles ◽  
In Vitro Maturation ◽  
Mrna Translation ◽  
Cumulus Cells ◽  
Oocyte Development ◽  
Follicle Cells ◽  
Biological Processes ◽  
Estrous Cycle Stage

Abstract Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. Extracellular vesicles are an important form of intercellular communication, since they carry bioactive contents, such as microRNAs (miRNAs), mRNAs, and proteins. MicroRNAs are small noncoding RNA capable of modulating mRNA translation. Thus, EVs can play a role in follicle and oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim of this study was to investigate the bovine miRNA content in EVs obtained from follicles at different estrous cycle stages, which are associated with different progesterone (P4) levels in the follicular fluid (FF). We collected FF from 3 to 6 mm follicles and evaluated the miRNA profile of the EVs and their effects on cumulus-oocyte complexes during in vitro maturation. We observed that EVs from low P4 group have a higher abundance of miRNAs predicted to modulate pathways, such as MAPK, RNA transport, Hippo, Cell cycle, FoxO, oocyte meiosis, and TGF-beta. Additionally, EVs were taken up by cumulus cells and, thus, affected the RNA global profile 9 h after EV supplementation. Cumulus cells supplemented with EVs from low P4 presented upregulated genes that could modulate biological processes, such as oocyte development, immune responses, and Notch signaling compared with genes of cumulus cells in the EV free media or with EVs from high P4 follicles. In conclusion, our results demonstrate that EV miRNA contents are distinct in follicles exposed to different estrous cycle stage. Supplementation with EVs impacts gene expression and biological processes in cumulus cells.

In vitro embryo production using ovaries removed from culled cows

2001 ◽  
Vol 81 (4) ◽  
pp. 589-591 ◽  
Author(s):  
G. Giritharan ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Estrous Cycle ◽  
Recovery Rate ◽  
In Vitro Maturation ◽  
Follicle Stimulating Hormone ◽  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

The objective was to utilize in vitro maturation and fertilization to maximize embryo production from culled dairy cows. Ovaries were removed (via a colopotomy technique) from culled Holstein cows (n = 21) 2 d after standing estrus (T1), 2 d after treatment with 40 mg follicle stimulating hormone (FSH) given at standing estrus (T2), or at an unknown stage of the estrous cycle (T3).Treatment with FSH increased (P < 0.05) oocyte recovery rate (mean ± SEM, 14.6 ± 2.2, 25.4 ± 3.4, and 11.1 ± 2.6 oocytes ovary–1), for groups T1, T2 and T3, respectively, and the number of blastocysts obtained (1.6 ± 0.5, 4.4 ± 0.4 and 1.6 ± 0.6 blastocysts ovary–1). Culled dairy cows of high genetic merit are potential embryo donors, with an average of six to eight transferable embryos obtained from each FSH-treated cow. Key words: FSH treatment, oocyte recovery, IVM, IVF, culled cows

Effect of isoflavone compounds on the in vitro maturation of sheep oocytes

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
AA Amir ◽  
GB Martin ◽  
JM Kelly ◽  
DO Kleemann ◽  
Z Durmic ◽  
...  
Keyword(s):  
In Vitro Maturation

Glucocorticoid metabolism in bovine cumulus-oocyte complex during in vitro maturation

2014 ◽  
Author(s):  
Masafumi Tetsuka ◽  
Ryo Takagi ◽  
Nobuhiro Ambo ◽  
Yuta Zempo ◽  
Asuka Onuma
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Oocyte Complex ◽  
Glucocorticoid Metabolism
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