136 Progesterone concentration during bovine in vitro maturation might serve as a predictor of oocyte developmental capacity

2022 ◽  
Vol 34 (2) ◽  
pp. 306
Author(s):  
J. Gutiérrez-Añez ◽  
P. Aldag ◽  
H. Niemann ◽  
A. Lucas-Hahn
Keyword(s):  
In Vitro Maturation ◽  
Progesterone Concentration ◽  
Developmental Capacity

Trehalose supplementation during porcine oocytes in vitro maturation improves the developmental capacity of parthenotes

2020 ◽  
Vol 141 ◽  
pp. 91-97
Author(s):  
Lian Cai ◽  
Yeon-Woo Jeong ◽  
Sang-Hwan Hyun ◽  
Il-Jeoung Yu ◽  
Woo-Suk Hwang ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Developmental Capacity

scholarly journals Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles

2019 ◽  
Vol 36 (10) ◽  
pp. 2135-2144 ◽  
Author(s):  
Flor Sanchez ◽  
Anh H. Le ◽  
Vu N. A. Ho ◽  
Sergio Romero ◽  
Heidi Van Ranst ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Antral Follicles ◽  
Developmental Capacity

Bovine oocytes treated prior to in vitro maturation with a combination of butyrolactone I and roscovitine at low doses maintain a normal developmental capacity

2001 ◽  
Vol 60 (4) ◽  
pp. 579-585 ◽  
Author(s):  
Nunzia Ponderato ◽  
Irina Lagutina ◽  
Gabriella Crotti ◽  
Paola Turini ◽  
Cesare Galli ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Low Doses ◽  
Developmental Capacity ◽  
Bovine Oocytes

Effect of hormone pre-treatment of prepubertal sheep on the production and developmental capacity of oocytes in vitro and in vivo

1997 ◽  
Vol 9 (6) ◽  
pp. 625 ◽  
Author(s):  
J. K. O'Brien ◽  
N. F. G. Beck ◽  
W. M. C. Maxwell ◽  
G. Evans
Keyword(s):  
In Vitro Maturation ◽  
Single Treatment ◽  
Oocyte Collection ◽  
Oestradiol Benzoate ◽  
Developmental Capacity ◽  
Pre Treatment ◽  
Pregnant Mare Serum Gonadotrophin

Twenty 36-week-old Merino lambs were given either 3, 1 or 0 treatments of 50 µg oestradiol benzoate and (48 h later) a 1·5 mg Norgestamet implant left in situ for 9 days (3-, 1- and 0CYCLE+G). On Day 7 after the last implant insertion, and on the same day for 0CYCLE+G, each lamb received 400 I.U. pregnant mare serum gonadotrophin and 6 mg follicle-stimulating hormone (FSH). The reproductive tracts were removed for oocyte collection 24 h after FSH. Reproductive tracts were also collected from 16-24-week-old lambs (n = 31) (0CYCLEG). The number of antral follicles per ovary was similar for the 3-, 1- and 0CYCLE+G treatments. Similar rates of in vitro maturation and monospermic fertilization were obtained for all groups. The proportion of blastocysts per cleaved oocyte was higher for 1CYCLE+G (50·5%) than for 3CYCLE+G (32·9%), 0CYCLE+G (24·3%), and 0CYCLEG (11·8%) (P < 0·05). Viable fetuses were obtained at Day 93 of pregnancy after transfer of embryos from all treatments. These results indicate that a single treatment with oestrogen and progesterone, prior to gonadotrophin stimulation, will increase the yield and developmental capacity of oocytes from prepubertal sheep.

Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity

2012 ◽  
Vol 24 (4) ◽  
pp. 568 ◽  
Author(s):  
Eva Held ◽  
Eva-Maria Mertens ◽  
Abdollah Mohammadi-Sangcheshmeh ◽  
Dessie Salilew-Wondim ◽  
Urban Besenfelder ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zona Pellucida ◽  
In Vitro Maturation ◽  
Immature Oocytes ◽  
Developmental Capacity ◽  
Bovine Oocytes ◽  
Scanning Electron

In the present study we aimed to analyse structural changes during in vitro maturation of the bovine zona pellucida (ZP) by scanning electron microscopy (SEM) ands zona pellucida birefringence (ZPB). Here we show that alterations during in vitro maturation invasively analysed by SEM are reflected in ZPB. In vivo-matured oocytes displayed significantly lower birefringence parameters and significantly higher blastocyst rates compared with in vitro-derived oocytes (39.1% vs 21.6%). The same was observed for in vitro-matured oocytes with cumulus–oocyte complex (COC) Quality 1 (Q1) compared with Q3-COCs with respect to zona birefringence and developmental capacity. Immature oocytes with Q1-COCs displayed higher ZPB values and a higher developmental capacity to the blastocyst stage (27.7% vs 16.9%) compared with immature Q3-COCs. Considering in vitro-matured oocytes, only those with Q1-COC showed a trend for ZPB similar to in vivo-matured oocytes. Therefore, a decreasing trend for ZPB during in vitro maturation seems to be typical for high-quality oocytes and successful cytoplasmic maturation. In accordance, fully-grown immature oocytes reached significantly higher blastocyst rates (32.0% vs 11.5%) and lower ZPB values compared with still-growing ones. In conclusion, we successfully evaluated the applicability of zona imaging to bovine oocytes: alterations during in vitro maturation invasively analysed by scanning electron microscopy were reflected in the birefringence of the zona pellucida of bovine oocytes affecting developmental capacity at the same value. Therefore ZPB measurement by live zona imaging has potential to become a new tool to assess correctness of in vitro maturation and to predict developmental competence.

scholarly journals Cumulus expansion and glucose utilisation by bovine cumulus–oocyte complexes during in vitro maturation: the influence of glucosamine and follicle-stimulating hormone

Reproduction ◽  
2004 ◽  
Vol 128 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Melanie L Sutton-McDowall ◽  
Robert B Gilchrist ◽  
Jeremy G Thompson
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Lactate Production ◽  
Glucose Consumption ◽  
Lactate Metabolism ◽  
Alternative Substrate ◽  
Glucose Incorporation ◽  
Developmental Capacity ◽  
Total Glucose

Glucose is an important metabolite and its presence during in vitro oocyte maturation (IVM) can have profound effects on the oocyte’s developmental capacity. We have demonstrated that glucose uptake increases over a 24 h IVM period, with most accounted for as l-lactate production. However, as maturation proceeds, l-lactate production remains constant, suggesting an alternative role for glucose metabolism. We hypothesised that in the latter stages of oocyte maturation, glucose not accounted for by l-lactate production is utilised for FSH-stimulated extracellular matrix (ECM) synthesis. To examine precursor utilisation for synthesis of ECM, bovine cumulus–oocyte complexes (COCs) were matured in ± FSH and/or glucosamine (an alternative substrate of matrix components). Measurements included COC diameters, glucose consumption and l-lactate production in spent media and [U-14C]glucose incorporation into ECM. FSH significantly stimulated both diameter and glucose consumption during 20–24 h maturation compared with unstimulated complexes, although co-incubation with glucosamine and FSH decreased total glucose consumption 1.7-fold compared with FSH alone (P < 0.05). Furthermore, there was a linear relationship between glucose and l-lactate metabolism in the presence of glucosamine, suggesting that the majority of glucose was being utilised for l-lactate production via glycolysis. In the presence of glucosamine, twofold less [U-14C]glucose was incorporated into matrix compared with COCs cultured without glucosamine. These results support the hypothesis that there is a link between glucose and glucosamine uptake in FSH-stimulated ECM synthesis. Furthermore, glucose has multiple fates within the COC during maturation and levels of utilisation are dependent on the composition of the maturation environment.

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