Bovine oocytes treated prior to in vitro maturation with a combination of butyrolactone I and roscovitine at low doses maintain a normal developmental capacity

2001 ◽  
Vol 60 (4) ◽  
pp. 579-585 ◽  
Author(s):  
Nunzia Ponderato ◽  
Irina Lagutina ◽  
Gabriella Crotti ◽  
Paola Turini ◽  
Cesare Galli ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Low Doses ◽  
Developmental Capacity ◽  
Bovine Oocytes

Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity

2012 ◽  
Vol 24 (4) ◽  
pp. 568 ◽  
Author(s):  
Eva Held ◽  
Eva-Maria Mertens ◽  
Abdollah Mohammadi-Sangcheshmeh ◽  
Dessie Salilew-Wondim ◽  
Urban Besenfelder ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zona Pellucida ◽  
In Vitro Maturation ◽  
Immature Oocytes ◽  
Developmental Capacity ◽  
Bovine Oocytes ◽  
Scanning Electron

In the present study we aimed to analyse structural changes during in vitro maturation of the bovine zona pellucida (ZP) by scanning electron microscopy (SEM) ands zona pellucida birefringence (ZPB). Here we show that alterations during in vitro maturation invasively analysed by SEM are reflected in ZPB. In vivo-matured oocytes displayed significantly lower birefringence parameters and significantly higher blastocyst rates compared with in vitro-derived oocytes (39.1% vs 21.6%). The same was observed for in vitro-matured oocytes with cumulus–oocyte complex (COC) Quality 1 (Q1) compared with Q3-COCs with respect to zona birefringence and developmental capacity. Immature oocytes with Q1-COCs displayed higher ZPB values and a higher developmental capacity to the blastocyst stage (27.7% vs 16.9%) compared with immature Q3-COCs. Considering in vitro-matured oocytes, only those with Q1-COC showed a trend for ZPB similar to in vivo-matured oocytes. Therefore, a decreasing trend for ZPB during in vitro maturation seems to be typical for high-quality oocytes and successful cytoplasmic maturation. In accordance, fully-grown immature oocytes reached significantly higher blastocyst rates (32.0% vs 11.5%) and lower ZPB values compared with still-growing ones. In conclusion, we successfully evaluated the applicability of zona imaging to bovine oocytes: alterations during in vitro maturation invasively analysed by scanning electron microscopy were reflected in the birefringence of the zona pellucida of bovine oocytes affecting developmental capacity at the same value. Therefore ZPB measurement by live zona imaging has potential to become a new tool to assess correctness of in vitro maturation and to predict developmental competence.

scholarly journals Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247518
Author(s):  
Thais Preisser Pontelo ◽  
Mauricio Machaim Franco ◽  
Taynan Stonoga Kawamoto ◽  
Felippe Manoel Costa Caixeta ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Embryo Development ◽  
In Vitro Maturation ◽  
Embryo Quality ◽  
Cumulus Cells ◽  
Developmental Competence ◽  
Nuclear Maturation ◽  
Deacetylase Inhibitor ◽  
Developmental Capacity ◽  
Bovine Oocytes

This study aimed to evaluate the effect of scriptaid during pre-maturation (PIVM) and/or maturation (IVM) on developmental competence of bovine oocytes. Cumulus-oocyte complexes (COCs) were submitted to PIVM for 6 h in the presence or absence of scriptaid. COCs were distributed into five groups: T1-IVM for 22 h, T2-PIVM for 6 h and IVM for 22 h, T3-PIVM with scriptaid for 6 h and IVM for 22 h, T4-PIVM for 6 h and IVM with scriptaid for 22 h, and T5-PIVM with scriptaid for 6 h and IVM with scriptaid for 22 h. Nuclear maturation, gene expression, cumulus cells (CCs) expansion, and embryo development and quality were evaluated. At the end of maturation, all groups presented the majority of oocytes in MII (P>0.05). Only HAT1 gene was differentially expressed (P<0.01) in oocytes with different treatments. Regarding embryo development at D7, T4 (23%) and T5 (18%) had lower blastocyst rate (P<0.05) than the other treatments (T1 = 35%, T2 = 37% and T3 = 32%). No effect was observed when scriptaid in PIVM was used in less competent oocytes (P>0.05). In conclusion, presence of scriptaid in PIVM and/or IVM did not improve developmental competence or embryo quality.

In vitro maturation and early developmental capacity of bovine oocytes cultured in pure follicular fluid and supplementation with follicular wall

2007 ◽  
Vol 67 (5) ◽  
pp. 1053-1059 ◽  
Author(s):  
Natalia V. Coleman ◽  
Galina A. Shagiakhmetova ◽  
Irina Y. Lebedeva ◽  
Tatiana I. Kuzmina ◽  
Alexander K. Golubev
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Developmental Capacity ◽  
Early Developmental ◽  
Bovine Oocytes

Development to the late morula or blastocyst stage following in vitro maturation and fertilization of bovine oocytes

1989 ◽  
Vol 18 (1-3) ◽  
pp. 139-148 ◽  
Author(s):  
Y. Fukui ◽  
M. Urakawa ◽  
C. Sasaki ◽  
N. Chikamatsu ◽  
H. Ono
Keyword(s):  
In Vitro Maturation ◽  
Blastocyst Stage ◽  
Bovine Oocytes
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