129 Effect of equine chorionic gonadotrophin doses on number and size of ovarian follicles at the time of oocyte collection in llamas

2022 ◽  
Vol 34 (2) ◽  
pp. 302
Author(s):  
U. H. Perez ◽  
F. W. Gutierrez ◽  
Y. M. Quispe ◽  
J. H. Melgar ◽  
N. Luque ◽  
...  
Keyword(s):  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Oocyte Collection

MATURATIONAL CHANGES IN SHEEP OVARIAN FOLLICLES: GONADOTROPHIC STIMULATION OF CYCLIC AMP PRODUCTION BY ISOLATED THECA AND GRANULOSA CELLS

1978 ◽  
Vol 89 (1) ◽  
pp. 166-172 ◽  
Author(s):  
T. J. Weiss ◽  
D. T. Armstrong ◽  
J. E. A. McIntosh ◽  
R. F. Seamark
Keyword(s):  
Cyclic Amp ◽  
Granulosa Cells ◽  
Follicle Stimulating Hormone ◽  
Adenosine Monophosphate ◽  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Camp Production ◽  
Stimulation Of ◽  
Stimulating Hormone

ABSTRACT Theca and granulosa tissues isolated from sheep ovarian follicles of different sizes were incubated in the presence of human chorionic gonadotrophin (HCG; 5 IU/ml) or follicle stimulating hormone (FSH; 5 μg NIH-FSH-S11/ml) for 40 min. Changes in the total amounts of cyclic 3′,5′-adenosine monophosphate (cAMP) were used as an index of the responsiveness of these preparations to the hormones. Thecal tissue of both large (4–6 mm in diameter) and small (1–3 mm) follicles responded similarly to gonadotrophins. Granulosa cells from small follicles failed to respond to stimulation by HCG. FSH, however, consistently increased cAMP production in comparison with controls or cells treated with HCG. Granulosa cells of large follicles responded to both HCG and FSH.

22 Strategies for oocyte collection procedures in free-roaming bison herds

2021 ◽  
Vol 33 (2) ◽  
pp. 118
Author(s):  
M. L. Zwiefelhofer ◽  
T. Shury ◽  
E. M. Zwiefelhofer ◽  
G. P. Adams
Keyword(s):  
Single Dose ◽  
Drug Administration ◽  
Multiple Dose ◽  
Transvaginal Ultrasound ◽  
Chorionic Gonadotrophin ◽  
The Other ◽  
Embryo Production ◽  
Oocyte Collection ◽  
Wood Bison ◽  
Time Required

The study was conducted to test the feasibility of protocols for field collection of cumulus–oocyte complexes (COC) for invitro embryo production (IVP) in wild bison. The study was done with captive wood bison during the late anovulatory season (July). In Experiment 1, bison were assigned randomly to 2 groups (n=8/group) in which transvaginal ultrasound-guided COC collection was done in a chute without sedation or in lateral recumbency after chemical immobilization using a dart gun to compare collection efficiencies. In Experiment 2, a 2×2 design was used to examine the effects of superstimulation treatments [single-dose equine chorionic gonadotrophin (eCG) vs. multiple-dose FSH] and methods of drug administration (manual injection vs. field darting) on COC collection and IVP. Initial COC collection was done to serve as a non-superstimulated random start and to synchronize follicular wave emergence on the following day (Day 0; n=16). Half of the bison were given a single dose of 5000IU of eCG intramuscularly (IM) on Day −1. The other half were given 200mg of FSH IM on Day 0 and Day 2, followed by 2000IU human chorionic gonadotrophin IM on Day 4. Superstimulatory treatments were given by manual injection (chute restrained) to half of the bison in each group and by field darting in the other half. The COC were collected on Day 4 in the eCG group and Day 5 in the FSH group. Recovered COC were matured invitro for 25 to 28h at 38.8°C, fertilized (2×106 sperm mL−1) and co-incubated at 38.8°C in 5% O2, 5% CO2, and 90% N2 for 18h. Presumptive zygotes were denuded and cultured at 38.8°C in 5% O2, 5% CO2, and 90% N2. A commercially available IVF media set was utilised (IVF Bioscience). Data were compared by ANOVA and GLIMMIX. In Experiment 1, no difference was detected between chute-restrained vs. chemically immobilized groups in the time required to complete COC collection (6.9±1.0 vs. 8.9±1.0 min; P=0.2), the number of follicles aspirated (11.5±1.9 vs. 9.3±1.8; P=0.4), or the COC recovery rate [COC recovered/follicle aspirated; 58/92 (63%) vs. 44/69 (64%); P=0.9]. In Experiment 2, no differences were detected between superstimulation treatments (eCG vs. FSH) or method of drug administration (manual injection vs. field darting) for any endpoint. The number of medium (4.5–7.5 mm; 9.5±1.0 vs. 2.5±0.6; P<0.0001) and large (>8 mm; 8.6±0.8 vs. 3.4±1.5; P=0.004) follicles available was greater after superstimulation (groups combined) than without superstimulation (random start). Although there was no difference in the number of recovered compact COC between the superstimulated and non-superstimulated bison (8.9±1.3 vs. 6.2±1.7; P=0.2), the embryo production rate (number of embryos produced/number of COC matured) was greater after superstimulation than without superstimulation [54/189 (39%) vs. 19/108 (18%); P<0.05]. We conclude that COC collection in a field setting is feasible and minimum-handling superstimulation with a single dose of eCG is as effective as a multiple-dose FSH protocol for the invitro production of embryos in bison. This research was supported by NSERC and Vetoquinol.

scholarly journals Fas and Fas ligand protein and mRNA in normal and atretic mouse ovarian follicles

Reproduction ◽  
2003 ◽  
pp. 783-789 ◽  
Author(s):  
SJ Dharma ◽  
RL Kelkar ◽  
TD Nandedkar
Keyword(s):  
In Situ Hybridization ◽  
Fas Ligand ◽  
Death Receptor ◽  
Underlying Mechanism ◽  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Follicular Atresia ◽  
Immature Female ◽  
Apoptotic Pathways

Apoptosis is the underlying mechanism of follicular atresia in the mammalian ovary. However, the apoptotic pathways governing this ovarian process are not completely elucidated. In the present study, expression of Fas and Fas ligand, the proximal members of the death receptor pathway, was evaluated in mouse ovarian follicles using immunofluorescence and in situ hybridization. Normal or atretic follicles were obtained from immature female Swiss mice after administration of 10 iu equine chorionic gonadotrophin for 48 or 72 h, respectively. Expression of both Fas and Fas ligand mRNA and protein was observed in granulosa cells of normal and atretic follicles. Although the oocytes of normal follicles failed to show any staining, those of atretic follicles stained intensely for Fas, indicating that the presence of Fas in the oocyte determines the fate of the follicle.

scholarly journals The optimal size of ovarian follicles at oocyte collection

2019 ◽  
Vol 111 (4) ◽  
pp. e32
Author(s):  
C.E. Bedient ◽  
L.A. Kaye ◽  
A. Raman ◽  
F.C. Garner ◽  
M. Aguirre ◽  
...  
Keyword(s):  
Ovarian Follicles ◽  
Optimal Size ◽  
Oocyte Collection

scholarly journals Inhibition of Cholesterol Biosynthesis in Ovine Ovarian Follicles in vitro by Human Chorionic Gonadotrophin

1978 ◽  
Vol 31 (4) ◽  
pp. 405 ◽  
Author(s):  
TJ Douglas ◽  
RP Hamilton ◽  
RF Seamark
Keyword(s):  
Cholesterol Biosynthesis ◽  
Mevalonic Acid ◽  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

Cholesterol biosynthesis from DL-[2-14C]mevalonic acid ([l4C]MVA) was demonstrated in ovine ovarian foIlicles and isolated thecal tissues and granulosaI ceIls incubated in vitro. Thecal tissues more readily synthesized cholesterol than did granulosal ceIls when incubated separately, but in the intact follicle the newly synthesized cholesterol distributed evenly between the two tissue layers, indicating that the theca could act as a supplementary source of cholesterol for the granulosal ceIls.

scholarly journals Oxygen Uptake, Glucose Utilization, Lactate Release and Adenine Nucleotide Content of Sheep Ovarian Follicles in Culture: Effect of Human Chorionic Gonadotrophin

1976 ◽  
Vol 29 (6) ◽  
pp. 557 ◽  
Author(s):  
RF Seamark ◽  
F Amato ◽  
Susan Hendrickson ◽  
RM Moor
Keyword(s):  
Oxygen Uptake ◽  
Glucose Utilization ◽  
Adenine Nucleotide ◽  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Lactate Release ◽  
Nucleotide Content ◽  
Wet Weight ◽  
The Mean

A study has been made of the oxygen uptake, glucose utilization, lactate release and cellular content of adenine nucleotides of isolated sheep ovarian follicles (4-6 mm in diameter) maintained in organ culture, and of the effects on these parameters of the addition of human chorionic gonadotrophin (hCG). The mean oxygen consumption of the entire follicles when freshly isolated and of the theca and membrana components was 0�56, 1�08 and 0�05 .umol per milligram wet weight of tissue per hour respectively. About 8.umol of glucose was taken up and 16 .umol of lactate released per milligram wet weight of follicular tissue per hour during the first 24-h period of culture. This rate reduced by about 30 % for each subsequent day of culture, but was significantly increased by the addition of hCG. The mean ATP content of theca and granulosa tissues was 4�6 and 2�8 nmol/mg wet weight of tissue respectively. There was no discernable change in tissue adenine nucleotide content or energy charge ratio during the 3-day culture period, and prolonged exposure to hCG was without effect. Untreated follicles produced both oestrogen and androgens throughout the culture period. The addition of hCG resulted in a transitory stimulation in oestrogen secretion, inhibition of androgen secretion, and a marked and sustained rise in progestin secretion.

Maturation of ovarian follicles after inhibition of ovulation in rats

1983 ◽  
Vol 97 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Osamu Mizuno ◽  
Tsuyoshi Otani ◽  
Mariko Shirota ◽  
Shuji Sasamoto
Keyword(s):  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Follicular Maturation ◽  
Progesterone Treatment ◽  
Preovulatory Follicles ◽  
Subsequent Cycle ◽  
Pentobarbitone Sodium

The present investigation was performed to elucidate the mechanism of the initiation of follicular maturation after inhibition of ovulation in rats treated with pentobarbitone sodium at 13.30 h and progesterone at 14.00 h on the day of pro-oestrus (day 0 denotes the day of these treatments). Ovulation was completely inhibited and the next spontaneous ovulation occurred on day 5, the expected day of the next oestrus. Follicular responsiveness to injection of human chorionic gonadotrophin (hCG) indicated that preovulatory follicles at the time of treatment with pentobarbitone and progesterone regressed by 05.00 h on day 2. Maturation of a new set of follicles began from 17.00 h on day 2 and all rats were induced to ovulate by hCG injection by 17.00 h on day 3, the number of oocytes ovulated being comparable to normal ovulation. In the animals receiving pentobarbitone sodium and progesterone treatment, two selective rises in plasma FSH, which had peak levels at 05.00 h on day 1 and 11.00 h on day 2, were observed without a rise in LH. Preovulatory surges of FSH and LH occurred on the afternoon of day 4. These results suggest that the second rise in FSH was induced by regression of Graafian follicles present at the time of treatment with pentobarbitone sodium and progesterone and that this surge of FSH was responsible for initiation of maturation of a new set of follicles destined to ovulate in the subsequent cycle. The mechanism of induction and the role of the first rise of FSH from the night of day 0 to the morning of day 1 cannot be explained at present.

scholarly journals Lipid Content of Sheep Ovarian Follicles in Culture

1978 ◽  
Vol 31 (6) ◽  
pp. 621
Author(s):  
RP Hamilton ◽  
RF Seamark
Keyword(s):  
Organ Culture ◽  
Lipid Content ◽  
Detailed Examination ◽  
Ovarian Follicles ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

A detailed examination was made of the lipid content of sheep ovarian follicles maintained in organ culture, and of the effect on this of human chorionic gonadotrophin.

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